71002-59-0

Upstream product

• 63660-22-0 Phosphoric acid 4-chloro-phenyl ester 2-cyano-ethyl ester (2R,3S,5R)-5-(2-isobutyrylamino-6-oxo-1,6-dihydro-purin-9-yl)-2-[(4-methoxy-phenyl)-diphenyl-methoxymethyl]-tetrahydro-furan-3-yl ester 
• 13388-88-0 4-chlorophenyl phosphate 
• 106-48-9 4-chloro-phenol 
• 772-79-2 4-chlorophenylphosphorodichloridate 
• 69507-62-6 (p-chlorophenyl) (β-cyanoethyl) phosphorochloridate 
• 68892-41-1 5'-O-dimethoxytrityl-N-isobutyryldeoxyguanosine 
• 71100-72-6 5'-O-(dimethoxytrityl)-N-isobutyrylguanine 3'-(p-chlorophenylcyanoethyl phosphate) 
• 76742-75-1 5'-O-monomethoxytrityl-N-isobutyryldeoxyguanosine 3'-p-chlorophenylphosphonate 

Downstream Products

• 81551-80-6 C₄₃H₄₇Cl₂N₁₁O₁₅P₂ 
• 90827-16-0 C₃₉H₄₂Cl₂N₈O₁₅P₂ 
• 69924-11-4 C₆₄H₆₅Cl₂N₁₁O₁₇P₂ 
• 73591-24-9 5'-O-(bis(4-methoxyphenyl)phenylmethyl)-P-(4-chlorophenyl)thymidylyl-(3'->5')-2'-deoxy-N-(2-methyl-1-oxopropyl)-3'-guanylic acid, 4-chlorophenyl 2-cyanoethyl ester 

Relevant academic research and scientific papers

Synthesis of Deoxyoligonucleotide Linker Fragments for Genetic Engineering Using Improved Preparative and Analytical Techniques

For studies on the expression of recombinant DNA, linker fragments have been prepared which serve to introduce specific points of cleavage on the DNA as well as the protein level.Such linkers were designed for cyanogen bromide as well as for collagenase cleavage of fused proteins.The preparation was done according to the triester synthesis scheme using improved and simpler routes to functionalized dinucleotide building blocks.Field desorption mass spectrometry was used as a routine tool for the identification and control of the purity of these units.

A SIMPLIFIED STRATEGY FOR THE SYNTHESIS OF DIDEOXYRIBONUCLEOTIDE BLOCKS

The rapid synthesis of dideoxyribonucleotide blocks in 60-85percent isolated yields has been achieved by combining the phosphorylation and condensation steps in a sequential reaction series which also allows the recovery of unreacted nucleotides.