75125-22-3Relevant academic research and scientific papers
Chemical Synthesis of the Pentadecadeoxyribonucleotide d(TTCTTCTACACACCC) by Improved Triester Approach
Rosenthal, A.,Cech, D.,Veiko, V. P.,Shabarova, Z. A.,Orezkaja, T. S.
, p. 764 - 773 (2007/10/02)
The chemical synthesis of the pentadecanucleotide d(TTCTTCTACACACCC) by improved triester approach in solution with TPS/1-Methylimidazole as condensation agent is reported.Almost all condensation were complete within 5-10 minutes giving yields over 80 percent.All intermediate triester blocks showed purity degrees of higher than 90 percent after purification by simple silica gel chromatography.After deblocking the pentadecanucleotide was purified by DEAE-cellulose 52 chromatography and desalted, then showing 100 percent purity as seen by 20 percent polyacrylamide gel electrophoresis of the 5'-32P labeled oligonucleotide.The sequence of the synthetic oligomer was determined and confirmed by using the two-dimensional mobility shift method.The pentadecanucleotide was used as a hybridisation probe for cloned human insulin c-DNA.
