7801-35-6Relevant academic research and scientific papers
One-step C-terminal deprotection and activation of peptides with peptide amidase from stenotrophomonas maltophilia in neat organic solvent
Arif, Muhammad I.,Toplak, Ana,Szymanski, Wiktor,Feringa, Ben L.,Nuijens, Timo,Quaedflieg, Peter J. L. M.,Wu, Bian,Janssen, Dick B.
, p. 2197 - 2202 (2014/07/21)
Chemoenzymatic peptide synthesis is a rapidly developing technology for cost effective peptide production on a large scale. As an alternative to the traditional C→N strategy, which employs expensive N-protected building blocks in each step, we have investigated an N→C extension route that is based on activation of a peptide C-terminal amide protecting group to the corresponding methyl ester. We found that this conversion is efficiently catalysed by Stenotrophomonas maltophilia peptide amidase in neat organic media. The system excludes the possibility of internal peptide cleavage as the enzyme lacks intrinsic protease activity. The produced peptide methyl ester was used for peptide chain extension in a kinetically controlled reaction by a thermostable protease.
Application of an organophosphorus reagent depbt for synthesis of cycloheptapeptide
Xie,Tian,Ye
, p. 4233 - 4240 (2007/10/03)
A cycloheptapeptide cyclo(Gly-Tyr-Gly-Gly-Pro-Phe-Pro), which was isolated and identified from one kind of Chinese medicinal herbs, was chosen as a model cyclopeptide, to evaluate an organophosphorus reagent, 3-(diethyloxyphosphoryloxy)-1,2,3-benzotriazin
