816429-78-4Relevant articles and documents
Metal-assisted assembly and stabilization of collagen-like triple helices
Cai, Weibo,Kwok, Sen Wai,Taulane, Joseph P.,Goodman, Murray
, p. 15030 - 15031 (2007/10/03)
Single-chain and TRIS-assembled collagen mimetic peptide structures incorporating catechol groups were synthesized. When 1/3 equiv of Fe3+ was added to the single-chain compound in 50 mM CAPS buffer (pH 10), the 1:3 Fe3+-catechol complex that formed acted as an N-terminal scaffold to assemble the triple helix. When 1 equiv of Fe3+ was added to the TRIS-assembled compound in the buffer solution, the Fe3+-catechol complex acted as an extra C-terminal scaffold, which lead to a triple helix with both termini tethered. The formation of this C-terminal complex increased the Tm by a remarkable 22 °C! Copyright