87919-08-2

Basic information

• Product Name: benzyl N-(6',6''-di-tert-butyl-18β-glycyrrhizinyl)-trans-4-aminomethylcyclohexanecarboxylate
• Synonyms: benzyl N-(6',6''-di-tert-butyl-18β-glycyrrhizinyl)-trans-4-aminomethylcyclohexanecarboxylate
• CAS NO: 87919-08-2
• Molecular Weight: 1164.48
• Mol File: 87919-08-2.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: benzyl N-(6',6''-di-tert-butyl-18β-glycyrrhizinyl)-trans-4-aminomethylcyclohexanecarboxylate(CAS DataBase Reference)
• NIST Chemistry Reference: benzyl N-(6',6''-di-tert-butyl-18β-glycyrrhizinyl)-trans-4-aminomethylcyclohexanecarboxylate(87919-08-2)
• EPA Substance Registry System: benzyl N-(6',6''-di-tert-butyl-18β-glycyrrhizinyl)-trans-4-aminomethylcyclohexanecarboxylate(87919-08-2)

Safety Data

• Hazardous Substances Data: 87919-08-2(Hazardous Substances Data)

Upstream product

• 50867-21-5 N,N'-dicylohexylcarbamimidic acid tert-butyl ester 
• 83896-44-0 glycyrrizhin 
• 87918-95-4 6',6'',30-tribenzyl-18β-glycyrrhizin 
• 87919-01-5 6',6''-di-tert-butyl-30-benzyl-18β-glycyrrhizin 
• 87918-99-8 30-benzyl-18β-glycyrrhizin 
• 87919-06-0 benzyl trans-4-(aminomethyl)cyclohexanecarboxylate tosylate 
• 87919-03-7 6',6''-di-tert-butyl-18β-glycyrrhizin 

Downstream Products

• 87919-12-8 hydroxysuccinimidyl N-(6',6''-di-tert-butyl-18β-glycyrrhizinyl)-trans-4-aminomethylcyclohexanecarboxylate 
• 87919-10-6 N-(6',6''-di-tert-butyl-18β-glycyrrhizinyl)-trans-4-aminomethylcyclohexanecarboxylic acid 

Relevant articles and documents

Studies on the Enzyme Immunoassay of Bio-active Constituents Contained in Oriental Medicinal Drugs. II. Enzyme Immunoassay of Glycyrrhizin

In order to develop an enzyme immunoassay of glycyrrhizin (GL), N-(glycyrrhizinyl)-ε-aminohexanoic acid and -trans-4-aminomethylcyclohexanecarboxylic acid were synthesized as haptens from GL via 6',6''-di-tert-butyl GL (VI) as a key intermediate, which was obtained by the selective tert-butylation of GL with O-tert-butyl-N,N'-dicyclohexylisourea or by the hydrogenolysis of 6',6''-di-tert-butyl-30-benzyl GL (V) over palladium carbon.Coupling of the hapten with bovine serum albumin (BSA) (carrier protein) and β-galactosidase (labelled enzyme) was carried out by the N-hydroxysuccinimide ester method.Anti-GL serum was elicited in rabbits by immunization with N-(glycyrrhizinyl)-ε-aminohexanoic acid-BSA conjugate (XIV).Separation of bound and free fractions was perfomed by a double antibody method using a goat antiserum to rabbit IgG. 7-β-D-Galactopyranosyl-4-methylcoumarin was used as the substrate for the fluorometric assay of β-galactosidase activity.A satisfactory standard curve for GL was obtained in the range of 0.2-20 ng/ml.Keywords --- enzyme immunoassay; glycyrrhizin; ε-aminohexanoic acid; trans-4-(aminomethyl)cyclohexanecarboxylic acid; N-hydroxysuccinimide ester method; O-tert-butyl-N,N'-dicyclohexylisourea; O-benzyl-N,N'-dicyclohexylisourea; β-galactosidase; 7-β-D-galactopyranosyloxy-4-methylcoumarin