93966-64-4

Upstream product

• 98-88-4 benzoyl chloride 
• 40615-39-2 5'-O-(4-4'-dimethoxytrityl)thymidine 
• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 50-89-5 thymidine 
• 98-07-7 Benzotrichlorid 
• 100-66-3 methoxybenzene 
• 40615-35-8 4,4'-dimethoxytrityl alcohol 
• 17331-53-2 3'-O-benzoylthymidine 

Downstream Products

• 17331-53-2 3'-O-benzoylthymidine 
• 400649-81-2 5'-O-(4,4'-dimethoxytrityl)-3'-O-benzoyl-(5S,6R)-5,6-dihydro-5,6-dihydroxythymidine 
• 318497-55-1 5'-O-(4,4'-dimethoxytrityl)-3'-O-benzoyl-(5R,6S)-5,6-dihydro-5,6-dihydroxythymidine 
• 116980-77-9 C₅₄H₅₃N₄O₁₄PS 
• 365-08-2 dTTP 
• 85240-01-3 C₆₁H₅₅ClN₇O₁₄P 
• 73118-61-3 Benzoic acid (2R,3S,5R)-2-[[(2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-hydroxymethyl-tetrahydro-furan-3-yloxy]-(2-chloro-phenoxy)-phosphoryloxymethyl]-5-(5-methyl-2,4-dioxo-3,4-dihydro-2H-pyrimidin-1-yl)-tetrahydro-furan-3-yl ester 
• 81187-04-4 C₅₄H₅₂ClN₄O₁₅P 
• 69940-70-1 C₃₃H₃₄ClN₄O₁₃P 
• 318497-56-2 Benzoic acid (2R,3S,5R)-5-[(5R,6S)-5,6-bis-(tert-butyl-dimethyl-silanyloxy)-5-methyl-2,4-dioxo-tetrahydro-pyrimidin-1-yl]-2-[bis-(4-methoxy-phenyl)-phenyl-methoxymethyl]-tetrahydro-furan-3-yl ester 
• 318497-57-3 (5R,6S)-1-{(2R,4S,5R)-5-[Bis-(4-methoxy-phenyl)-phenyl-methoxymethyl]-4-hydroxy-tetrahydro-furan-2-yl}-5,6-bis-(tert-butyl-dimethyl-silanyloxy)-5-methyl-dihydro-pyrimidine-2,4-dione 
• 400649-82-3 5'-O-(4,4'-dimethoxytrityl)-3'-O-benzoyl-(5S,6R)-5,6-dihydro-5,6-di[(tert-butyl)dimethylsilyloxy]thymidine 
• 400649-83-4 5'-O-(4,4'-dimethoxytrityl)-(5S,6R)-5,6-dihydro-5,6-di[(tert-butyl)dimethylsilyloxy]thymidine 
• 400649-85-6 5'-O-(4,4'-dimethoxytrityl)-3'-O-benzoyl-(5R,6S)-5,6-dihydro-5-hydroxy-6-(tert-butyl)dimethylsilyloxythymidine 

Relevant academic research and scientific papers

PHOTORESPONSIVE DEOXYRIBONUCLEOSIDE TRIPHOSPHATE

PROBLEM TO BE SOLVED: To provide photoresponsive deoxyribonucleoside triphosphate in which the initiation of the amplification reaction of a target nucleic acid can be controlled by light irradiation, and to provide its production precursor. SOLUTION: The above problem is solved by the compound represented by the general formula (1) (where, R1 and R2 each independently represent an alkyl group having 1 to 8 carbon atoms; R3 and R4 independently represent a hydrogen atom, an alkyl group having 1 to 6 carbon atoms, or a phenyl group; R5 represents a hydrogen atom or a group represented by the formula (7) [-P(=O)(OH)-O-P(=O)(OH)-O-P(=O)(OH)2(7)]; Y represents a hydrogen atom or a hydroxy group; and X represents a nucleobase). SELECTED DRAWING: None COPYRIGHT: (C)2022,JPOandINPIT

COMPOSITIONS AND METHODS FOR SYNTHESIS OF PHOSPHORYLATED MOLECULES

The invention provides compositions and methods for synthesis of phosphorylated organic compounds, including nucleoside triphosphates.

Renewable amberlyst-15 catalyzed highly regioselective tritylation and deprotection of sugar-based diols

Amberlyst-15 catalyzed highly regioselective tritylation of sugar-based diols was achieved under mild condition using 4,4′-dimethoxytrityl alcohol (DMTrOH). Deprotection of the corresponding DMTr group was also established by the variation to protic solvent. Meanwhile, the heterogeneous catalyst Amberlyst-15 was recycled 3 times with satisfactory retention of catalytic activity and proved its potential application in industry.

A versatile one-pot procedure to phosphate monoesters and pyrophosphates using Di(p-methoxybenzyl)-N,N-diisopropylphosphoramidite

(Chemical Equation Presented) A one-pot procedure for the preparation of phosphoramidates, phosphorothioates, pyrophosphates, phosphodiesters, and phosphofluoridates has been devised using di(p-methoxybenzyl)-N,N- diisopropylphosphoramidite as the common phosphitylating reagent.

Nucleotide libraries as a source of biologically relevant chemical diversity: Solution-phase synthesis

Solution-phase parallel synthesis of nucleotide library 1 consisting of 150 members is reported. (C) 2000 Elsevier Science Ltd. All rights reserved.

Synthesis and Application of a New Fluorescein Derivative for Fluorescent Labelling of Oligonucleotides and as a Novel Tool for Non-Radioactive DNA Sequencing

The synthesis of 4(5)-(bromomethyl)fluorescein, which can be used as its 3',6'-dibenzoyl derivative 7 for the labelling of nucleosides is described.Pyrimidine nucleosides such as 3'- and/or 5'-protected 2'-deoxyuridine and thymidine are labelled by 7 at N3 of the heterocyclic base.The resulting base-labelled 3'-benzoyl-5'-dimethoxytrityl nucleosides may, on the one hand, be converted, after cleavage of the protective dimethoxytrityl group, into the 5'-phosphoramidite 11 by normal phosphorylation; on the other hand, 5'-triphosphates 13 are prepared by phosphorylation with 2-chloro-4H-1,3,2-benzodioxaphosphorin-4-one and pyrophosphate.The phosphoramidites 11 were sucessfully used for the fluorescent labelling of oligonucleotides during automatic DNA synthesis.The oligonucleotides can be used as primer in automatic non-radioactive DNA sequencing.The 5'-triphosphates 13 are potential substrates for DNA polymerases or terminal transferase. - Key Words: Xanthenes / Fluorescein labelling / Dyes / Phosphoramidite synthesis / Oligonucleotides / DNA sequencing

SELF-COMPLEMENTARY TETRADEOXYRIBONUCLEOSIDE TRIPHOSPHATES. CONVENIENT CHEMICAL PREPARATION AND SPECTROSCOPIC STUDIES IN SOLUTION

Eight kinds of self-complementary tetradeoxyribonucleoside triphosphates were prepared by a simplified method which enabled us to omit purification of synthetic intermediates and provided the tetramers very rapidly and conveniently.The tetramers were characterized by enzyme assay and their conformations were studied by the use of UV and CD spectroscopic methods under various conditions.The detailed analysis of the CD spectra suggested that conformation of the tetramer duplexes was dependent on the sequence.

Simplified Liquid-Phase Preparation of Four Decadeoxyribonucleotides and their Preliminary Spectroscopic Characterization

Four self-complementary decadeoxyribonucleotides, dApTpApTpApTpApTpApT,dApTpApTpCpGpApTpApT, dApTpApTpGpCpApTpApT and dApApApApApTpTpTpTpT, were chemically synthesized by the phosphotriester procedure.Efforts were concentrated on keeping the procedure as simple as possible, concomitant with obtaining high-purity products at each step of the process.The decamers were elaborated from the 3'-end, starting with a 3'-O-benzoylated monomer according to the scheme: monomer + monomer -> dimer + dimer -> tetramer + dimer -> hexamer + tetramer -> decamer.Putity of intermediates and of the fully blocked decamers were monitored by chromatography and by 300-MHz 1H-NMR. spectroscopy.The deblocked decadeoxyribonucleotides were characterized by their UV., CD., and 1H-NMR. spectra.