Reference

Computer-assisted, high-performance liquid chromatography with mass spectrometric detection for the analysis of coumarins in Peucedanum palustre and Angelica archangelica

Computer-assisted, high-performance liquid chromatography with mass spectrometric detection for the analysis of coumarins in Peucedanum palustre and Angelica archangelica. Eeva, Manu; Rauha, Jussi-Pekka; Vuorela, Pia; Vuorela, Heikki ( Faculty of Pharmacy, Division of Pharmacognosy, University of Helsinki, Helsinki FIN-00014, Finland). Phytochemical Analysis, 15(3), 167-174 (English) 2004 John Wiley & Sons Ltd. CODEN: PHANEL. ISSN: 0958-0344. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Section cross-reference(s): 11 A reversed-phase HPLC method with atm. pressure chem. ionization MS detection has been developed for the sepn. and identification of coumarins in plants of Peucedanum palustre L. (Moench) and Angelica archangelica (L.) var. archangelica. The Turbo Method Development program was utilized to optimize the mobile phase with two org. solvents (acetonitrile and methanol) and two aq. solns. (1.0% formic acid and 10 mM ammonium acetate). Optimization of the solvent gradients for the method was performed with the aid of the DryLab program. Analyses were carried out using a Phenomenex Prodigy RP C18 column. Fifty-two peaks (14 of which were assocd.Several substances with their cas registry numbers 3866-13-5 and 484-12-8 may be metioned in this study. with coumarins) were sepd. in 30 min from exts. of P. palustre, and 48 peaks (15 assocd. with coumarins) from exts. of A. archangelica. A total of 21 different coumarin-type compds. were identified in the aerial and the underground parts of the title plants. Isopimpinellin and pimpinellin were found for the first time in P. palustre and were identified by comparison of retention times and MS data obtained following the anal. of pure stds. This is the first report of the coumarin compn. of the umbels of P. palustre. .

Chemical aspects of coumarin compounds for the prevention of hepatocellular carcinomas

Chemical aspects of coumarin compounds for the prevention of hepatocellular carcinomas. Okamoto, Toshihiro; Kobayashi, Tadashi; Yoshida, Shinichi (Research Laboratories, Nippon Chemiphar Co., Ltd, Saitama 341-0005, Japan). Current Medicinal Chemistry: Anti-Cancer Agents, 5(1), 47-51 (English) 2005 Bentham Science Publishers Ltd. CODEN: CMCACI. 484-12-8 and 91-64-5 are also occured in this study. ISSN: 1568-0118. DOCUMENT TYPE: Journal; General Review CA Section: 1 (Pharmacology) Section cross-reference(s): 14 A review. The normalization of plasma alanine aminotranferase (ALT) has been proved to be a strategy for preventing the development of hepatocellular carcinoma (HCC) in hepatitis C virus (HCV) infection. Glycyrrhizin, a plant medicine, normalizes plasma ALT and prevents HCC. However, glycyrrhizin is administered i.v. and thereby chem. which is effective on oral administration is required. Coumarin compds. are active components of herbs used for the treatment of various diseases. The ability of coumarin compds. to lower plasma ALT were examd. using mice Con A-induced hepatitis and mice anti-Fas antibody-induced hepatitis. Furanocoumarins pd-Ia, pd-II, and pd-III lower plasma ALT, but they are large mols. that are hardly absorbed on oral administration. Furocoumarin effectively lowers plasma ALT, but the safety range between the effective and toxic dosages is narrow. In contrast, osthole, a simple coumarin, causes strong redn. of plasma ALT and also inhibits caspase-3 activation. Furthermore, this chem. is quite safe upon large dose administration. In the structure of osthole, the methoxy group at position-7 and the 3-methyl-2-butenyl group at position-8 were elucidated to be essential for the beneficial effect of this chem. Thus, osthole will become a leading chem. for synthesizing a compd. which prevents HCC on oral administration. .