Reference

Characterization of a potent inhibitor of platelet aggregation and release reaction isolated from Allium sativum (garlic)

Characterization of a potent inhibitor of platelet aggregation and release reaction isolated from Allium sativum (garlic). Mohammad, S. Fazal; Woodward, Stephen C. (Sch. Med., Univ. Utah, Salt Lake City, UT 84132, USA). Thromb. Res., 44(6), 793-806 (English) 1986. CODEN: THBRAA. ISSN: 0049-3848. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) When added to platelet-rich plasma, aq. exts. of garlic inhibited platelet aggregation and the release reaction. Subsequent expts. designed to characterize the inhibitory component revealed that the inhibitory activity was assocd. with low-mol.-wt. components, the inhibitory component possessed the typical garlic odor and contained an abundance of S, the inhibitory activity could be extd. with org. solvents, and temps. >56° and alk. pH >8.5 quickly destroyed the inhibitory activity. The Rf value of the major inhibitory component after thin-layer chromatog. was similar to that of allicin [539-86-6], a unique thiosulfinate in garlic previously shown to possess strong antibiotic and antifungal properties. Allicin was synthesized. On thin-layer chromatog., allicin comigrated with the inhibitory component in garlic. At 10 mM, allicin inhibited completely platelet aggregation and the release reaction. Comparative studies suggest that the major platelet aggregation and release inhibitor in garlic may be allicin.

Evaluation of the in vitro antifungal activity of allicin

Evaluation of the in vitro antifungal activity of allicin. Yamada, Yasuo; Azuma, Keizo (Sch. Med. Technol., Fujita-Gakuen Univ., Aichi, Japan). Antimicrob. Agents Chemother., 11(4), 743-9 (English) 1977. CODEN: AMACCQ. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Interactions) Allicin [539-86-6] was effective in vitro against Candida, Cryptococcus, Trichophyton, Epidermophyton, and Microsporum, but was significantly less effective against Aspergillus. The minimal inhibitory concn. of allicin against various pathogenic fungi was affected considerably by differences in the exptl. conditions, e.g., incubation time, inoculum size, type of medium, and medium pH. Allicin inhibited both germination of spores and growth of hyphae.