Reference

D-Fructose-mediated stimulation of bovine lens aldose reductase activation by UV-irradiation

D-Fructose-mediated stimulation of bovine lens aldose reductase activation by UV-irradiation. Mizoguchi, Tadashi; Ogura, Takeharu; Yagi, Kiyohito; Kador, Peter F. ( Faculty of Pharmaceutical Sciences, Osaka University, Suita 565, Japan). Advances in Experimental Medicine and Biology, 414(Enzymology and Molecular Biology of Carbonyl Metabolism 6), 529-535 (English) 1997 Plenum. CODEN: AEMBAP. ISSN: 0065-2598. DOCUMENT TYPE: Journal CA Section: 8 (Radiation Biochemistry) D-Fructose-mediated stimulation of bovine lens aldose reductase activation by UV-irradn. has been studied with respect to cataract formation.

Kinetic Characterization of Apical D-Fructose Transport in Chicken Jejunum

Kinetic Characterization of Apical D-Fructose Transport in Chicken Jejunum. Garriga, C.; Barfull, A.; Planas, J. M. (Pharmacology Faculty, Division IV, Department of Physiology, University of Barcelona, Barcelona E-08028, Spain). Journal of Membrane Biology, 197(1), 71-76 (English) 2004 Springer-Verlag New York Inc. CODEN: JMBBBO. ISSN: 0022-2631. DOCUMENT TYPE: Journal CA Section: 12 (Nonmammalian Biochemistry) In mammals, D-fructose transport takes place across the brush-border membrane of the small intestine through GLUT5, a member of the facilitative glucose transporter family. In the present paper, the authors describe and characterize for the first time the apical transport of D-fructose in chicken intestine. Brush-border membrane vesicles (BBMV) were obtained from jejunum of 5- to 6-wk-old chickens. D-Fructose uptake by BBMV from chicken jejunum comprises a saturable component and a simple diffusion process. The maximal rate of transport (Vmax) for D-fructose was 2.49 nmol/mg protein/s, the Michaelis const. (Km) was 29 mM, and the diffusion const. (Kd) was 25 nL/mg protein/s. The apical transport of D-fructose was Na+-independent, phlorizin-, phloretin-, and cytochalasin B-insensitive, and did not show cis-inhibition by D-glucose or D-galactose. These properties, together with the detection of specific GLUT5 mRNA, indicate the presence of a low-affinity high-capacity GLUT5-type carrier in the chicken jejunum, responsible for the entry of D-fructose across the brush-border membrane of enterocytes.