Reference

Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning

Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning. Han, Jang H.; Stratowa, Christian; Rutter, William J. (Hormone Res. Inst., Univ. California, San Francisco, CA 94143, USA). Biochemistry, 26(6), 1617-25 (English) 1987. CODEN: BICHAW. ISSN: 0006-2960. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 13 A full-length putative rat pancreatic lysophospholipase [9001-85-8] cDNA was cloned by an improved mRNA isolation method and cDNA cloning strategy. A low-temp. RNA extn. method using guanidinium thiocyanate [593-84-0] was developed. The cDNA cloning strategy uses versatile synthetic primer-adaptors contg. both homopolymer tails for the primed synthesis of cDNA and preformed restriction sites for cloning into the l phage vector. 106587-94-4 and 106587-90-0 which are cas registry numbers of chemicals are mentioned. These new methods allow the construction of a cDNA library from the adult rat pancreas in which the majority of recombinant clones contained complete sequences for the corresponding mRNAs. A previously recognized but unidentified long relatively rare cDNA clone contg. the entire sequence from the cap site at the 5' end to the poly (A) tail at the 3' end of the mRNA was isolated by single-step screening of the library. The size, amino acid compn., and the activity of the protein expressed in heterologous cells strongly suggest this mRNA codes for lysophospholipase. .

Odorless foam rubber articles expanded by dinitrosopentamethylenetetramine

Odorless foam rubber articles expanded by dinitrosopentamethylenetetramine. Alicot, Michel; Tignol, Adrien (Produits Chimiques Ugine Kuhlmann, Fr.). Fr. Demande FR 2354360 6 Jan 1978, 12 pp. (French). (France). CODEN: FRXXBL. CLASS: IC: C08J009-06. APPLICATION: FR 76-17714 11 Jun 1976. DOCUMENT TYPE: Patent CA Section: 38 (Elastomers, Including Natural Rubber) Odorless, open- or closed-cell foams of S-vulcanized natural or synthetic rubber are prepd. by blowing with dinitrosopentamethylenetetramine (I) [101-25-7] in the presence of guanidine nitrate (II) [506-93-4] or guanidine thiocyanate [593-84-0]. Thus, vulcanizing compounded SBR contg. 4 phr I and 4 phr II at 157° gives a foam with odor essentially none, cell structure fine and regular, and surface smooth, compared with pronounced and nauseating, fine and regular, and smooth, resp., in the absence of II, and pronounced and nauseating, irregular, and blistered, resp., with urea in place of II.