Reference

Galactosyl-insulin conjugates useful in treating diabetics

Galactosyl-insulin conjugates useful in treating diabetics. Myhre, David Vernon; Geho, Walter Blair (Procter and Gamble Co., USA). Eur. Pat. Appl. EP 119650 A2 26 Sep 1984, 17 pp. DESIGNATED STATES: R: AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: IC: A61K037-26. APPLICATION: EP 84-200328 8 Mar 1984. PRIORITY: US 83-477555 21 Mar 1983. DOCUMENT TYPE: Patent CA Section: 63 (Pharmaceuticals) Section cross-reference(s): 2 Galactosylinsulin conjugates, useful in the treatment of insulin [9004-10-8] dependent diabetics, consists of 31 D-galactosyl group covalently bonded to insulin. The D-galactosyl group targets the conjugates to the hepatocytes. When administered to diabetics the conjugates cause a more effective amt. of insulin to be delivered to the liver to control blood glucose levels. E.g., 20 g lactobionic acid [96-82-2] in 10 mL piperidine was stirred with 1.2 g DCC at 0° for 0.5 h. N-Hydroxysuccinimide [6066-82-6] (0.64 g) was added to give N-hydroxysuccinimide lactobionate [96607-63-5]. This lactobionate (0.6 g) was reacted with 6000 units of insulin to give lactobionylinsulin conjugates. This conjugate decreased blood glucose in diabetic dogs.

Determination of steroid hormone glucuronides

Determination of steroid hormone glucuronides. March, Steven Carl (Abbott Laboratories, USA). Eur. Pat. Appl. EP 114614 A2 1 Aug 1984, 18 pp. DESIGNATED STATES: R: BE, DE, FR, GB, IT. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: IC: G01N033-74; G01N033-54. APPLICATION: EP 84-100249 12 Jan 1984. PRIORITY: US 83-460739 25 Jan 1983. DOCUMENT TYPE: Patent CA Section: 2 (Mammalian Hormones) An enzyme immunoassay for urinary pregnanediol glucuronide (I) is described. Anti-I antibodies raised in a goat are used and the prepn. of the I tracer soln. comprising I covalently attached to alk. phosphatase is described. Thus, I is treated with N-hydroxysuccinimide [6066-82-6], 1-hydroxybenztriazole [2592-95-2], and N,N-dicyclohexylcarbodiimide [538-75-0] in DMF to form the I active ester which is then reacted with alk. phosphatase in DMF in M ratios of , , (I/alk. phosphatase). In the assay procedure a urine sample is mixed with a portion of the I tracer soln. and a std. vol. of I-antibody soln. and incubated at 37° for 10 min. A double-antibody precipitin suspension comprising normal goat serum and porcine antigoat serum is then added and the mixt. is incubated an addnl. 10 min. After centrifugation aliquots of the supernatant are treated with an alk. phosphatase reagent incubated at room temp. for 15 min and the absorbance of the sample is read at a wavelength suitable for p-nitrophenol and compared to a std. curve.