Reference

Effect of isoproterenol and EGTA on the volume-pressure relationship of the in vitro whole bladder preparation

Effect of isoproterenol and EGTA on the volume-pressure relationship of the in vitro whole bladder preparation. Levin, Robert M.; Goldman, Michele; Wein, Alan J. (Sch. Med., Univ. Pennsylvania, Philadelphia, PA 19104, USA). Neurourol. Urodyn., 3(2), 133-9 (English) 1984. CODEN: NEUREM. ISSN: 0733-2467. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) An in vitro whole bladder (rabbit) system was used to measure the effects of isoproterenol [7683-59-2] and EGTA [67-42-5] on bladder vol. while intravesical pressure was maintained at a const. level. At 3 mM, EGTA had only a slight effect on intravesical pressure, but it increased bladder vol. by ~80%. Isoproterenol had only a slight effect on pressure, but it produced a progressive increase in intravesical vol. with increasing concn.; the max. increase was ~60% and the Kd ~30 nM.

Polymorphonuclear leukocytic inhibition by citrate, other metal chelators, and trifluoperazine

Polymorphonuclear leukocytic inhibition by citrate, other metal chelators, and trifluoperazine. Evidence to support calcium binding protein involvement. Pfister, Roswell R.; Haddox, Jeffrey L.; Dodson, Robert W.; Deshazo, William F. (Eye Res. Lab., Brookwood Med. Cent., Birmingham, AL 35209, USA). Invest. Ophthalmol. Visual Sci., 25(8), 955-70 (English) 1984. CODEN: IOVSDA. ISSN: 0146-0404. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) In vitro studies demonstrated that the stimulation of human PMN by opsonized zymosan can be inhibited by citrate [77-92-9], EDTA [60-00-4], and EGTA [67-42-5]. These compds. interfere with opsonized zymosan attachment to PMN, preventing the respiratory burst, phagocytosis, and degranulation. Reversal of this inhibition by Ca and/or Mg suggests the mechanism is Ca chelation. Trifluoperazine (TFP) [117-89-5] inhibition of opsonized zymosan attachment and phagocytosis implicates the involvement of calmodulin. Thus, citrate, EDTA, and EGTA may interfere with the receptor mediated attachment of opsonized zymosan to the PMN cell membrane, leaving the PMN in a resting, granulated state. Inhibition of the receptor system by Ca depletion may be the result of interference with Ca-calmodulin modulated microfilament and/or microtubule interfaces in the PMN plasma membrane. It is postulated that comparable events occur in the citrate treated alkali burned cornea. Citrate inhibition of PMN may be useful in other eye and systemic diseases.