96-26-4Relevant articles and documents
Design of a synthetic enzyme cascade for the: In vitro fixation of a C1carbon source to a functional C4sugar
Güner, Samed,Pick, André,Sieber, Volker,Wegat, Vanessa
supporting information, p. 6583 - 6590 (2021/09/10)
Realizing a sustainable future requires intensifying the waste stream conversion, such as converting the greenhouse gas carbon dioxide into value-added products. In this paper, we focus on utilizing formaldehyde as a C1 carbon source for enzymatic C-C bond formation. Formaldehyde can be sustainably derived from other C1 feedstocks, and in this work, we designed a synthetic enzyme cascade for producing the functional C4 sugar erythrulose. This involved tailoring the enzyme formolase, which was optimized for fusing formaldehyde, from a three-carbon producer (dihydroxyacetone) to sets of variants with enhanced two-carbon (glycolaldehyde) or four-carbon (erythrulose) activity. To achieve this, a high-throughput combinatorial screening was developed, and every single variant was evaluated in terms of glycolaldehyde, dihydroxyacetone and erythrulose activity. By applying the two most promising variants in an enzyme cascade, we were able to show for the first time production of ERY starting from a C1 carbon source. In addition, we demonstrated that one of our tailored formolase variants was able to convert 25.0 g L-1 glycolaldehyde to 24.6 g L-1 erythrulose (98% theoretical yield) in a fully atom-economic biocatalytic process. This represents the highest achieved in vitro concentration of erythrulose to date.
One-pot biotransformation of glycerol into serinol catalysed by biocatalytic composites made of whole cells and immobilised enzymes
Ripoll, Magdalena,Velasco-Lozano, Susana,Jackson, Erienne,Diamanti, Eleftheria,Betancor, Lorena,López-Gallego, Fernando
supporting information, p. 1140 - 1146 (2021/02/26)
Biocatalytic cascades afford the development of economically sustainable and green processes. Herein we examined the unprecedented coupling of co-immobilisedGluconobacter oxydansand an isolated transaminase to synthesise serinol from glycerol. Through this approach, we manufactured up to 36 mM serinol, the highest titer ever reported for a non-fermentative biosynthesis. More importantly, similar productivities are obtained starting from the industrial by-product crude glycerol, demonstrating the possibilities of this hybrid heterogenenous biocatalyst for valorising bio-based raw materials.
Assembly of platinum nanoparticles and single-atom bismuth for selective oxidation of glycerol
Huang, Ning,Jiang, Dong,Jiang, Pingping,Leng, Yan,Lu, Yubing,Tian, Jinshu,Yue, Chenguang,Zhang, Pingbo,Zhang, Zihao
supporting information, p. 25576 - 25584 (2021/12/07)
Selective oxidation of the secondary hydroxyl group of glycerol to dihydroxyacetone (DHA) is an extremely challenging yet important reaction. The main difficulty is that the three hydroxyl groups in glycerol are prone to randomly oxidize, resulting in an unsatisfactory DHA selectivity. We show here that an assembly of platinum nanoparticles (NPs, ~2 nm) and N-stabilized single-atom bismuth (Bi), namely Pt/Bi@NC, shows a record-high DHA selectivity of ~95.0% towards glycerol oxidation under optimized reaction conditions. Characterization and theoretical calculations confirm that single-atom Bi in the vicinity of Pt NPs provides a preferred site to chelate with the primary -OH of glycerol, and its secondary -OH is prone to bind to a surface Pt atom of a Pt NP with a shorter Pt-H bond length. This as-formed unique adsorption configuration of glycerol on the Pt-Bi dual site significantly facilitates the oxidation of the secondary -OH of glycerol, thus contributing to a record-high selectivity to DHA. This journal is