11075-17-5

Basic information

• Product Name: CARBOXYPEPTIDASE A
• Synonyms: Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase from bovine pancreas;Carboxypeptidase A from bovine pancreas,Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase;Peptidyl-L-amino-acid hydrolase from bovine pancreas;Carboxypeptidase A?Agarose;E.C. 3.4.12.2;E.C. 3.4.2.1;PEPTIDYL-L-AMINO-ACID HYDROLASE;PEPTIDYL-L-AMINO-ACID HYDROLASE TYPE II-PMSF
• CAS NO: 11075-17-5
• Molecular Formula: C₄H₁₁NO
• Molecular Weight: 0
• EINECS: 234-297-5
• Mol File: 11075-17-5.mol

Chemical Properties

• Flash Point: 4 °C
• Appearance: white/suspension
• Density: 1.00 g/mL at 20 °C
• Storage Temp.: 2-8°C
• CAS DataBase Reference: CARBOXYPEPTIDASE A(CAS DataBase Reference)
• NIST Chemistry Reference: CARBOXYPEPTIDASE A(11075-17-5)
• EPA Substance Registry System: CARBOXYPEPTIDASE A(11075-17-5)

Safety Data

• Hazard Codes: F,Xn,B,Xi
• Statements: 11-38-42-48/20-63-65-67-36-36/37/38
• Safety Statements: 36/37-45-62-36-26-23-16-24-22
• RIDADR: UN 1294 3/PG 2
• WGK Germany: 2
• F: 10
• Hazardous Substances Data: 11075-17-5(Hazardous Substances Data)

Usage

Uses

Used in Research Applications:
Carboxypeptidase A is used as a comparison tool for studying the specificity of other enzymes, such as Metarhizium anisopliae carboxypeptidase A (MeCPA). This helps in understanding the differences in their catalytic properties and potential applications.
Used in Protein Engineering:
CPA has been utilized in the genetic engineering process to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. This is crucial for the purification and characterization of recombinant proteins in various biotechnological applications.
Used in Cell Biology:
Carboxypeptidase A is used to de-tyrosinate α-tubulin in vitro, which helps in inducing high affinity to ethyl-N-phenylcarbamate (EPC) sepharose. This process is essential for studying the structure and function of tubulin proteins and their interactions with other cellular components.
Used in Digestive System:
As a part of the digestive system, Carboxypeptidase A is used as an enzyme that cleaves amino acids favoring the C-terminal end, as well as certain esters. This enzymatic activity plays a vital role in the breakdown and absorption of proteins in the body.
Used in Enzyme Production:
The Carboxypeptidase A-agarose product, prepared by immobilizing CPA to activated 4% crosslinked beaded agarose, is used in various industrial applications, such as enzyme production, where the immobilized enzyme can be easily separated from the reaction mixture and reused multiple times, making the process more efficient and cost-effective.

Biochem/physiol Actions

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.

Purification Methods

Carboxypeptidase A is purified by DEAE-cellulose chromatography, activation with trypsin and dialysed against 0.1M NaCl, yielding crystals. It is recrystallised by dissolving in 20 mL of M NaCl and dialysed for 24hours each against the following salts present in 500mL of 0.02M sodium veronal pH 8.0, 0.5M NaCl, 0.2M NaCl and 0.15M NaCl. The last dialysate usually induces crystallisation. If it does not crystallise, then dialyse the last solution against 0.02M sodium veronal containing 0.10M NaCl. Only 2 or 3 recrystallisations are required to attain maximum activity. [Cox et al. Biochemistry 3 44 1964.] Enzyme activity is measured by hydrolysing hippuryl-L-phenylalanine (or phenylacetic acid) and observing the rate of change of optical density at 254nm (reaction extinction coefficient is ~0.592 cm2/Wmole at pH 7.5) [Bergmyer Methods in Enzymatic Analysis (Academic Press) 1 436 1974].

Upstream product

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• 94-36-0 dibenzoyl peroxide 
• 98069-33-1 ethyl-carbamic acid-(2-chloro-ethyl ester) 
• 141-43-5 ethanolamine 
• 106-93-4 ethylene dibromide 
• 543-24-8 N-acetylglycine 
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• 16250-70-7 2-methyl-2,3,4,5-tetrahydroisoxazole 
• 108-24-7 acetic anhydride 
• 17886-23-6 1-ethyl-4,5-dihydro-1H-[1,2,3]triazole 

Related news

Application of CARBOXYPEPTIDASE A (cas 11075-17-5) to a baking process to mitigate contamination of wheat flour by ochratoxin A07/19/2019

This study aimed at evaluating an enzymatic strategy to mitigate ochratoxin A (OTA) exposure via contaminated wheat flour. Firstly, the effect of Rhizopus oryzae and Trichoderma reesei, which are both non-toxigenic microorganisms, on OTA, was evaluated. The mycotoxin ​​was degraded by both fungi...detailed

Cloning, characterization and transmission blocking potential of midgut CARBOXYPEPTIDASE A (cas 11075-17-5) in Anopheles stephensi07/18/2019

Transmission-blocking vaccines (TBV) interrupt malaria parasite transmission and hence form an important component for malaria eradication. Mosquito midgut exopeptidases such as aminopeptidase N & carboxypeptidase B have demonstrated TBV potential. In the present study, we cloned and characteriz...detailed

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