1132-61-2

Basic information

• Product Name: 3-Morpholinopropanesulfonic acid
• Synonyms: MOPS;MOPS, CONCENTRATE SOLUTION;MORPHOLINOPROPANE SULFONIC ACID;TIMTEC-BB SBB009133;LABOTEST-BB LT00455082;LABOTEST-BB LT01595956;4-MORPHOLINEPROPANESULFONIC ACID;4-(MORPHOLINOPROPANE SULFONIC ACID)
• CAS NO: 1132-61-2
• Molecular Formula: C₇H₁₅NO₄S
• Molecular Weight: 209.26
• EINECS: 214-478-5
• Product Categories: Miscellaneous Biochemicals;Organic acids;Biochemistry;Good's Buffers;Buffer;Imidazoles
• Mol File: 1132-61-2.mol

Chemical Properties

• Melting Point: 277-282 °C
• Flash Point: 116 °C
• Appearance: White/Powder/Solid
• Density: 1.3168 (rough estimate)
• Vapor Pressure: 0Pa at 25℃
• Refractive Index: 1.6370 (estimate)
• Storage Temp.: Store at RT.
• Solubility: H2O: 1 M at 20 °C, clear
• PKA: 7.2(at 25℃)
• Water Solubility: 1000 g/L (20 ºC)
• Stability: Stable. Incompatible with strong bases, strong oxidizing agents.
• Merck: 14,6265
• BRN: 1106776
• CAS DataBase Reference: 3-Morpholinopropanesulfonic acid(CAS DataBase Reference)
• NIST Chemistry Reference: 3-Morpholinopropanesulfonic acid(1132-61-2)
• EPA Substance Registry System: 3-Morpholinopropanesulfonic acid(1132-61-2)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36
• WGK Germany: 1
• RTECS: QE9104530
• TSCA: Yes
• Hazardous Substances Data: 1132-61-2(Hazardous Substances Data)

Usage

Uses

Used in Cell Culture:
3-Morpholinopropanesulfonic acid is used as a cell culture additive component in lentiviral particle production. It helps maintain the pH of the mammalian cell culture medium, ensuring optimal growth conditions for the cells.
Used in Microbial Growth Medium:
MOPS is used as a buffering agent in microbial growth medium, providing a stable pH environment for the growth and metabolism of microorganisms.
Used in Nuclei Extraction Buffer:
3-Morpholinopropanesulfonic acid is used as a buffering agent in nuclei extraction buffer, helping to maintain the pH during the process of isolating nuclei from cells.
Used in RPMI Medium:
MOPS is used as a component of Roswell Park Memorial Institute (RPMI) medium for diluting fungal inoculum, ensuring a stable pH for fungal growth.
Used in Capillary-Zone Electrophoresis:
3-Morpholinopropanesulfonic acid is used as a buffer in capillary-zone electrophoresis to test performance, providing a stable pH environment for the separation of molecules.
Used in Algal Sample Processing:
MOPS is used for the dilution of proteins from algal samples, maintaining a stable pH during the process.
Used in Denaturing Gel Electrophoresis of RNA:
3-Morpholinopropanesulfonic acid functions to maintain pH in denaturing gel electrophoresis of RNA, ensuring accurate separation and analysis of RNA molecules.
Used in Lipid Interactions and Membrane Properties:
MOPS can modify lipid interactions and influence the thickness and barrier properties of membranes, making it useful in studies involving membrane structure and function.
Used in Protein Stabilization:
3-Morpholinopropanesulfonic acid interacts with bovine serum albumin and stabilizes the protein, making it a useful component in protein storage and handling.
Used in Polyacrylamide Gel Electrophoresis:
MOPS is frequently used as a buffering agent in biology and biochemistry, including polyacrylamide gel electrophoresis, providing a stable pH environment for the separation of proteins or nucleic acids.

Reference

P. H. Quail, D. Marme, E. Sch?fer, Particle-bound phytochrome from maize and pumpkin, Nature New Biology, 1973, vol. 245, pp. 189-191

Flammability and Explosibility

Notclassified

InChI:InChI=1/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)

Synthetic route

morpholine (110-91-8) + 1,3-propanesultone (1120-71-4) → 3-(N-morpholino)propanesulfonic acid (1132-61-2)

Conditions	Yield
In acetone at 25℃; for 3.5h;	96%
In ethanol; water at 0℃; for 2h; Large scale;	81%

3-(N-morpholino)propanesulfonic acid (1132-61-2) → 3-(morpholin-4-yl)propane-1-sulfonyl chloride hydrochloride

Conditions	Yield
With thionyl chloride In N-methyl-acetamide
With thionyl chloride In N,N-dimethyl-formamide at 20℃; for 5h;

3-(N-morpholino)propanesulfonic acid (1132-61-2) + D-glucose (50-99-7) + caesin, acid hydrolyzed + extract of yeast + soy peptone, type SL + L-<3-(13)C>serine (89232-77-9) → C46(13)C15H60N14O18S5

Conditions	Yield
With Amycolatopsis fastidiosa MA7344 In water at 32℃; for 168h; Microbiological reaction;

3-(N-morpholino)propanesulfonic acid (1132-61-2) → C2H5O4S(1-)*C2H6NO3S(1-)*C7H14NO4S(1-)*3C7H15N2(1+)

Conditions	Yield
Multi-step reaction with 2 steps 1: water 2: water View Scheme

3-(N-morpholino)propanesulfonic acid (1132-61-2) + N-methyltriethylenediamine hydroxide → [C1ted][MOPS] (1416049-31-4)

Conditions	Yield
In water

3-(N-morpholino)propanesulfonic acid (1132-61-2) + tetra-n-butylphosphonium hydroxide (14518-69-5) → [TBP][MOPS]

Conditions	Yield
In water at 20℃;

3-(N-morpholino)propanesulfonic acid (1132-61-2) + tetramethyl ammoniumhydroxide (75-59-2) → C7H14NO4S(1-)*C4H12N(1+)

Conditions	Yield
In water at 20℃; for 12h;

3-(N-morpholino)propanesulfonic acid (1132-61-2) + tetraethylammonium hydroxide (77-98-5) → C7H14NO4S(1-)*C8H20N(1+)

Conditions	Yield
In water at 20℃; for 12h;

3-(N-morpholino)propanesulfonic acid (1132-61-2) + tetra(n-butyl)ammonium hydroxide (2052-49-5) → 3-Morpholin-4-yl-propane-1-sulfonatetetrabutyl-ammonium; (113599-00-1)

Conditions	Yield
In water at 20℃; for 12h;

Upstream product

• 110-91-8 morpholine 
• 1120-71-4 1,3-propanesultone 

Downstream Products

• 197891-91-1 3-(morpholin-4-yl)propane-1-sulfonyl chloride hydrochloride 

Relevant articles and documents

Preparation method of biological buffer agent--moropholino ethanesulfonic acid (MOPS)

The invention belongs to the field of organic synthesis and specifically relates to a preparation method of a biological buffer agent--moropholino ethanesulfonic acid (MOPS), comprising the followingstep: morpholine and 1,3-propane sultone (1,3-PS) react to generate moropholino ethanesulfonic acid. The raw materials used in the method are cheaper than raw materials used in the method for synthesizing moropholino ethanesulfonic acid by using 1,3-dibromopropane and morpholine, and the method of the invention is beneficial to large-scale production and application. by using ethanol and the likeas a solvent, the solvent is easy to remove, thus meeting the requirements of users on solvent residue. In the method, a reducing agent is added and nitrogen protection method is adopted for decoloration, thus ensuring chroma of MOPS and making the properties of the product to be better. The preparation process is simple and is convenient to operate and has high practicality.

Stabilizing labeled antibody using amino acids

The present invention relates to a method for stabilizing a labeled antibody in a solution, in which the labeled antibody is stabilized by allowing the labeled antibody to be present together with at least one of amino acid and a derivative thereof in the solution.

Aromatic compounds Mannich reaction using economical acidic ionic liquids based on morpholinium salts as dual solvent-catalysts

Economical acidic ionic liquids containing an alkanesulfonic acid group in a morpholinium cation were found to be effective catalysts for the Mannich reactions of various kinds of aromatic aldehydes, acetophenone and aromatic amines at mild reaction conditions. The satisfactory results were obtained with short reaction time, good yields of the Mannich bases and simplicity in the experimental procedure. These ionic liquids could be recycled and reused for up to five times without considerable decreasing in the catalytic activity. Copyright

CARBON MEMBRANE HAVING BIOLOGICAL MOLECULE IMMOBILIZED THEREON

Disclosed is a biological molecule-immobilized carbon membrane which comprises a porous carbon membrane and a biological molecule (e.g., an enzyme) immobilized on the carbon membrane, wherein the porous carbon membrane has three-dimensional cancellous pores through which fluid can permeate. The carbon membrane can have a large amount of a biological molecule (e.g., an enzyme) immobilized thereon and can also have a higher level of enzymatic activity or the like compared to a conventional one. Therefore, the carbon membrane is useful as an electrode for a bio-sensor or a bio-fuel cell.

Isolation of nucleic acids

A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.

Isolation of nucleic acids

A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.