- Preparation method of biological buffer agent--moropholino ethanesulfonic acid (MOPS)
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The invention belongs to the field of organic synthesis and specifically relates to a preparation method of a biological buffer agent--moropholino ethanesulfonic acid (MOPS), comprising the followingstep: morpholine and 1,3-propane sultone (1,3-PS) react to generate moropholino ethanesulfonic acid. The raw materials used in the method are cheaper than raw materials used in the method for synthesizing moropholino ethanesulfonic acid by using 1,3-dibromopropane and morpholine, and the method of the invention is beneficial to large-scale production and application. by using ethanol and the likeas a solvent, the solvent is easy to remove, thus meeting the requirements of users on solvent residue. In the method, a reducing agent is added and nitrogen protection method is adopted for decoloration, thus ensuring chroma of MOPS and making the properties of the product to be better. The preparation process is simple and is convenient to operate and has high practicality.
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Paragraph 0023-0027; 0030-0033
(2019/07/04)
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- Stabilizing labeled antibody using amino acids
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The present invention relates to a method for stabilizing a labeled antibody in a solution, in which the labeled antibody is stabilized by allowing the labeled antibody to be present together with at least one of amino acid and a derivative thereof in the solution.
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- Aromatic compounds Mannich reaction using economical acidic ionic liquids based on morpholinium salts as dual solvent-catalysts
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Economical acidic ionic liquids containing an alkanesulfonic acid group in a morpholinium cation were found to be effective catalysts for the Mannich reactions of various kinds of aromatic aldehydes, acetophenone and aromatic amines at mild reaction conditions. The satisfactory results were obtained with short reaction time, good yields of the Mannich bases and simplicity in the experimental procedure. These ionic liquids could be recycled and reused for up to five times without considerable decreasing in the catalytic activity. Copyright
- Yue, Caibo
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experimental part
p. 3640 - 3647
(2010/12/25)
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- CARBON MEMBRANE HAVING BIOLOGICAL MOLECULE IMMOBILIZED THEREON
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Disclosed is a biological molecule-immobilized carbon membrane which comprises a porous carbon membrane and a biological molecule (e.g., an enzyme) immobilized on the carbon membrane, wherein the porous carbon membrane has three-dimensional cancellous pores through which fluid can permeate. The carbon membrane can have a large amount of a biological molecule (e.g., an enzyme) immobilized thereon and can also have a higher level of enzymatic activity or the like compared to a conventional one. Therefore, the carbon membrane is useful as an electrode for a bio-sensor or a bio-fuel cell.
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- Isolation of nucleic acids
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A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.
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- Isolation of nucleic acids
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A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.
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