7365-45-9 Usage
Description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Chemical Properties
White crystalline powder
Uses
Different sources of media describe the Uses of 7365-45-9 differently. You can refer to the following data:
1. Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
2. HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.HEPES has been used as a component of:Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.
Definition
ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.
General Description
HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.
Biological Activity
Different sources of media describe the Biological Activity of 7365-45-9 differently. You can refer to the following data:
1. HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
2. Multi purpose buffer used in biological research.
Purification Methods
Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]
Check Digit Verification of cas no
The CAS Registry Mumber 7365-45-9 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 7,3,6 and 5 respectively; the second part has 2 digits, 4 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 7365-45:
(6*7)+(5*3)+(4*6)+(3*5)+(2*4)+(1*5)=109
109 % 10 = 9
So 7365-45-9 is a valid CAS Registry Number.
InChI:InChI=1/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)/p+1
7365-45-9Relevant articles and documents
Preparation method of piperazine ethanesulfonic acid derivative (by machine translation)
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Paragraph 0048-0087, (2020/02/06)
The reaction is carried out in such a manner that the reaction proceeds relatively, more thoroughly by reducing the side, reaction of the reaction system, in a manner such that the reaction proceeds relatively more thoroughly from, the system to 2 - a certain extent 2 - by reducing the reaction proceeds, to relatively more thoroughly; NaOH, NaCl, and. (by machine translation)
Method for preparing high-purity 4-hydroxyethylpiperazine ethanesulfonic acid
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Paragraph 0034; 0035; 0047, (2017/07/19)
The invention belongs to the technical field of preparation of organic matters, and relates to a method for preparing high-purity 4-hydroxyethylpiperazine ethanesulfonic acid. The method sequentially comprises the following specific steps: preparation of 4-hydroxyethylpiperazine ethanesulfonate, acidizing, extraction for decolorizing and removing impurities, reverse extraction, nanofiltration and washing, and drying, so that the high-purity 4-hydroxyethylpiperazine ethanesulfonic acid is finally obtained. The 4-hydroxyethylpiperazine ethanesulfonic acid prepared with the method provided by the invention is high in purity; the operation process is simple and convenient; the production process is environmentally friendly; the purification cost is low; the yield is high; the method is suitable for industrial batch production.
Peptides for Treatment of Obesity
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, (2013/03/26)
The present invention relates to novel peptide compounds which are effective in modulating one or more melanocortin receptor types, to the use of the compounds in therapy, to methods of treatment comprising administration of the compounds to patients in need thereof, and to the use of the compounds in the manufacture of medicaments. The compounds of the invention are of particular interest in relation to the treatment of obesity as well as a variety of diseases or conditions associated with obesity.