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CAS No.: | 7365-45-9 |
---|---|
Name: | HEPES |
Article Data: | 10 |
Molecular Structure: | |
Formula: | C8H18N2O4S |
Molecular Weight: | 238.308 |
Synonyms: | 1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonicacid;2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid;4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid;N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic acid;NSC 166663;N'-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid);N'-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid;TVZ 7;WAS 13;HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid);1-Piperazineethanesulfonicacid, 4-(2-hydroxyethyl)-; |
EINECS: | 230-907-9 |
Density: | 1.325 g/cm3 |
Melting Point: | 234-238 °C |
Boiling Point: | 408℃[at 101 325 Pa] |
Solubility: | soluble in water |
Appearance: | White crystalline powder |
Hazard Symbols: | Xi |
Risk Codes: | 36/37/38 |
Safety: | 24/25-22-36-26 |
PSA: | 89.46000 |
LogP: | -0.55930 |
1-(2-hydroxyethyl)piperazine
sodium 2-hydroxyethanesulfonate
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
Conditions | Yield |
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Stage #1: 1-(2-hydroxyethyl)piperazine; sodium 2-hydroxyethanesulfonate In methanol at 50℃; for 5.5h; Stage #2: With 5percent by weight of raffinate In water Solvent; Temperature; | 85.6% |
1-(2-hydroxyethyl)piperazine
Na-salt of 2-chloroethane-1-sulfonic acid
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
Conditions | Yield |
---|---|
In water at 85 - 105℃; for 0.5h; pH=10; Temperature; | 84.7% |
1-(2-hydroxyethyl)piperazine
ammonium vinylsulfonate
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
Conditions | Yield |
---|---|
Stage #1: 1-(2-hydroxyethyl)piperazine; ammonium vinylsulfonate In water at 60℃; for 4h; Stage #2: With sulfuric acid at 0℃; for 1h; |
1-(2-hydroxyethyl)piperazine
sodium vinylsulfonate
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
Conditions | Yield |
---|---|
Stage #1: 1-(2-hydroxyethyl)piperazine; sodium vinylsulfonate In water at 60 - 120℃; for 3h; Stage #2: With oxalic acid at 20℃; for 1h; Temperature; Reagent/catalyst; |
1-(2-hydroxyethyl)piperazine
potassium vinyl sulfonate
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
Conditions | Yield |
---|---|
Stage #1: 1-(2-hydroxyethyl)piperazine; potassium vinyl sulfonate In water at 50℃; for 3.5h; Stage #2: With sulfuric acid at 40℃; for 1h; |
1-(2-hydroxyethyl)piperazine
ethenesulfonic acid
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
Conditions | Yield |
---|---|
Stage #1: 1-(2-hydroxyethyl)piperazine; ethenesulfonic acid With sodium hydroxide In water at 40℃; for 3.5h; Stage #2: With oxalic acid at 20℃; for 1h; Temperature; Reagent/catalyst; |
1,1′-(1,4-butanediyl)bis(2-methylbenzimidazole)
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
Conditions | Yield |
---|---|
In ethanol mixt. of Ag salt, acid and imidazole ligand was stirred at room temp. for 30 min; ammonia added dropwise; slowly evapd. at room temp.; elem. anal.; | 68% |
2,3,4,5-tetrahydro-2-oxo-1,5-ethanolbenzazepine
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
(N,N-dimethylamino)ethyl 1,2,3,4-tetrahydroquinoline-4-propanoate
Conditions | Yield |
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In water at 25℃; Rate constant; |
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
A
Glyoxal
Conditions | Yield |
---|---|
With (batho)2CuII (batho = 2,9-dimethyl-4,7-diphenyl-1,10-phenanthrolinedisulfonate); water at 25℃; for 24h; Rate constant; Mechanism; pH 8; other substituted ethylenediamine; |
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
A
2'-deoxyguanosine 5'-monophosphate
Conditions | Yield |
---|---|
With sodium chloride; magnesium chloride; polycytidylate In water at 23℃; for 480h; pH=7.85; Kinetics; Product distribution; Further Variations:; Reagents; Condensation; dimerization; oligomerization; hydrolysis; |
1.Introduction of HEPES
The HEPES, with its CAS NO 7365-45-9, is a kind of white crystalline powder. It has synonyms of 1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonicacid;2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid;4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid and N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic acid. HEPES should be stored in shady and cool warehouse and mainly used as biological buffer.
2.Properties of HEPES
(1) H bond acceptors: 6 (2) H bond donors: 2 (3) Freely Rotating Bonds: 6
(4) Polar Surface Area: 75.22 Å2 (5) Index of Refraction: 1.543 (6) Molar Refractivity: 56.69 cm3
(7) Molar Volume: 179.7 cm3 (8) Surface Tension: 53.9 dyne/cm (9) Density: 1.325 g/cm3
(10) Melting Point: 234-238 °C (11) Storage temp: 2-8°C (12) Solubility: H2O: 1 M at 20 °C, clear, colorless
3.Sturture descriptors of HEPES
IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
InChI: InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,
(H,12,13,14)
InChIKey: JKMHFZQWWAIEOD-UHFFFAOYSA-N
Canonical SMILES : C1CN(CCN1CCO)CCS(=O)(=O)O
4.Uses of HEPES
HEPES is widely used in cell culture, mainly because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES is like water in that its dissociation decreases as the temperature decreases. This makes HEPES a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
5.Safety information of HEPES
Hazard Codes: Xi
Risk Statements: 36/37/38
R36/37/38: Irritating to eyes, respiratory system and skin.
Safety Statements: 24/25-22-36-26
S22: Do not breathe dust.
S24/25: Avoid contact with skin and eyes.
S26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S36: Wear suitable protective clothing.
WGK Germany: 1
RTECS: TL6809000
HS Code: 29335995
6.Toxity data of HEPES
Organism | Test Type | Route | Reported Dose (Normalized Dose) | Effect | Source |
---|---|---|---|---|---|
quail | LD50 | oral | > 316mg/kg (316mg/kg) | Ecotoxicology and Environmental Safety. Vol. 6, Pg. 149, 1982. |