Formation of Interribonucleoside Phosphate Bond by the Use of Reagent Formed by the Reaction of 2-Chlorophenyl Phosphorodichloridate with 5-Nitrobenzotriazole and Preparation of Anticodon Triplet of Yeast tRNALys

Article abstract of DOI:10.1246/cl.1987.775

The reagent formed by the reaction of 2-chlorophenyl phosphorodichloridate with an equimolar amount of 5-nitrobenzotriazole was treated with protected ribonucleosides having free 3'-OH, followed by protected ribonucleosides having free 5'-OH in pyridine for 8 h (the second step), the corresponding dinucleoside phosphates being obtained in 55-84 percent yields.When the second step was carried out in the presence of 2,6-lutidine, the yields were increased to 80-90 percent within 2 h.By this procedure, the anticodon triplet of yeast tRNA^Lys was prepared in 71 percent yield.