Synthesis, inhibitory effects on nitric oxide and structure-activity relationships of a glycosphingolipid from the marine sponge Aplysinella rhax and its analogues

Article abstract of DOI:10.3390/molecules16010637

The novel glycosphingolipid, β-D-GalNAcp(1→4)[α-D- Fucp(1→3)]-(3-D-GlcNAcp (1→Cer (A), isolated from the marine sponge Aplysinella rhax has a unique structure, with D-fucose and N-acetyl-D- galactosamine moieties attached to a reducing-end N-acetyl-D-gluc

Full text of DOI:10.3390/molecules16010637

Molecules 2011, 16, 637-651; doi:10.3390/molecules16010637
OPEN ACCESS
molecules
ISSN 1420-3049
www.mdpi.com/journal/molecules
Article
Synthesis, Inhibitory Effects on Nitric Oxide and Structure-
Activity Relationships of a Glycosphingolipid from the Marine
Sponge Aplysinella rhax and Its Analogues
Yuzo Fujita 1, Naohiro Ohshima 1, Ai Hasegawa 1, Frank Schweizer 2, Tadahiro Takeda 1,
Fumiyuki Kiuchi 1 and Noriyasu Hada 1,*
1
Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan
2
Department of Chemistry and Medical Microbiology, University of Manitoba, Winnipeg, Manitoba,
R3T 2N2, Canada
* Author to whom correspondence should be addressed; E-Mail: hada-nr@pha.keio.ac.jp;
Tel.: +81-3-5400-2666; Fax: + 81-3-5400-2556.
Received: 9 December 2010; in revised form: 29 December 2010 / Accepted: 14 January 2011 /
Published: 17 January 2011
Abstract: The novel glycosphingolipid, β-D-GalNAcp(1→4)[α-D-Fucp(1→3)]-β-D-
GlcNAcp(1→)Cer (A), isolated from the marine sponge Aplysinella rhax has a unique
structure, with D-fucose and N-acetyl-D-galactosamine moieties attached to a reducing-end
N-acetyl-D-glucosamine through an α1→3 and β1→4 linkage, respectively. We
synthesized glycolipid 1 and some non-natural di- and trisaccharide analogues 2-6
containing a D-fucose residue. Among these compounds, the natural type showed the most
potent nitric oxide (NO) production inhibitory activity against LPS-induced J774.1 cells.
Our results indicate that both the presence of a D-Fucα1-3GlcNAc-linkage and the
ceramide aglycon portion are crucial for optimal NO inhibition.
Keywords: glycosphingolipid; Aplysinella rhax; D-fucose; nitric oxide
1. Introduction
Carbohydrates in the form of glycoconjugates, for example glycoproteins, glycolipids and
proteoglycans, play an important role in many intracellular and extracellular events including cell-cell

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adhesion, cell differentiation, signal transduction, cancer metastasis and immune responses [1]. The
majority of these studies have focused on higher animals and relatively little is known about the
functions of glycoconjugates in lower animals [2]. In order to study the biological properties of
glycans in glycoconjugates, over the past decade we have synthesized novel glycolipid and
glycoprotein derivatives found in various invertebrates [3-12]. Organic synthesis is a powerful method
to explore structure activity relationships by providing access to large amounts of homogeneous and
structurally defined oligosaccharides including not only natural compounds, but also non-natural
compounds [13]. Recently, Zollo et al. isolated and characterized a novel neutral glycosphingolipid (A,
Figure 1) from the marine sponge Aplysinella rhax which features a D-fucose and an N-acetyl-D-
galactosamine attached to a reducing-end N-acetyl-D-glucosamine through a α1→3 and a β1→4
linkages, respectively [14]. This was the first report on glycolipids containing D-fucose. Furthermore,
these glycolipids have been found to exhibit significant inhibitory activity on LPS-induced nitric oxide
(NO) release by J774.1 macrophages. In order to study the structure-activity relationships of these
compounds inhibiting NO release, we previously reported the synthesis of β-D-GalNAcp(1→4)[α-D-
Fucp(1→3)]-β-D-GlcNAcp(1→)aglycon trisaccharide analogues, containing a 2-branched fatty alkyl
residue and a 2-(trimethylsilyl)ethyl (TMS-Et) residue, respectively [6]. Moreover, biological
evaluation of these novel glycosphingolipid analogues using an LPS-induced NO release assay
demonstrated that the presence of D-fucose is crucial for the NO inhibitory effect, while structural
modifications at the aglycon moiety appeared to have little to no effect on LPS-induced NO release [6].
In this study, we describe for the first time the total synthesis of glycosphingolipid 1 and its structural
analogues 3-6 to elucidate the structure activity relationships on LPS-induced NO production in more
detail (Figure 1).
Figure 1. Target glycosphingolipid and the analogue compounds
HO OH
HO
O
O
OCer
O
O
HO
NHAc
Me
NHAc
HO
O
HO
A
OH
O
HO
HO
OH
O
C13H27 HO
OH
O
HN
HO
O
HO OH
OH
O
HO
O
HO
O
O
O
O
C13
H
O
O
27 HO
TMS
O
TMS
OH
O
O
O
HO
OH
NHAc
Me
NHAc
Me
NHAc
Me
NHAc
HO
OH
O
O
NHAc
HO
O
HO
O
HO
HO
OH
HO
OH
HO
3
OH
2
1
HO
Me
O
OH
HO
HO
HO
HO
O
O
O
O
HO
O
TMS
O
Me
C13H27
C13H27
O
O
HN
NHAc
Me
TMS
OH
NHAc
Me
HO
O
HO
HO
O
O
O
HO
O
HO
HO
OH
HO
TMS
OH
NHAc
OH
4
6
7
5
2-(Trimethylsilyl)ethyl β-D-galactopyranosyl-(1→4)-[α-D-fucopyranosyl-(1→3)]-2-acetamido-2-
deoxy-β-D-glucopyranoside (3) was selected to explore how the presence of a terminal β-D-galacto-

Molecules 2011, 16
639
pyranosyl linkage instead of a 2-acetamido-2-deoxy-β-D-galactopyranosyl linkage affects the
biological effect. Disaccharide-based regioisomers 4 and 5 were selected to explore differences in the
connectivity of the α-D-fucopyranosyl moiety to the-β-D-GlcNAc portion while trisaccharide 6 was
chosen to study the effect of two α-D-fucopyranosyl linkages linked to the core -D-GlcNAc moiety.
The NO-inhibitory affect of commercially available ceramide 7 and known trisaccharide 2 was
included in these experiments for comparison.
2. Results and Discussion
2.1. Chemical synthesis
Synthesis of glycosphingolipid 1: Glycosylation of phytoceramide acceptor 9 [15] with the glycosyl
imidate 8 [6] was carried out in the presence of trimethylsilyl trifluoromethanesulfonate (TMSOTf)
[16] and 4 Å molecular sieves (MS4 Å) to obtain the desired glycolipid derivative 10 in 33% yield
with complete β-steroselectivity. Deprotection of the Troc group was achieved with Zn in a mixture
containing acetic anhydride and acetic acid, followed by catalytic hydrogenolysis over 10% Pd/C in
MeOH/THF to provide 11 in 47% yield. Deacetylation of 11 using Zemplén conditions and
purification by column chromatography on Sephadex LH-20 afforded target glycolipid 1 quantitatively
(Scheme 1).
Scheme 1. Synthesis of glycosphingolipid 1.
O
C13H27
HN
OBn
C13H27
O
OR3
HO
R3O
R3O
OAc
O
AcO
AcO
R3O
OR2
AcO
O O
C13H27
C13H27
HN
OBn
9
O
O
O
O
O
O
TMSOTf
NHR1
R3O
NHR1
Me
TrocHN
TrocHN
CCl3
NH
O
OR2
Me
O
O
MS4A
CH2Cl2
AcO
R3O
AcO
3
OAc
OR
8
R1
10 Troc
R2
Bn
R3
Ac 33%
1) Zn/AcOH, Ac2O
2) Pd-C/H2, MeOH, THF
11
1
Ac
Ac
H
H
Ac 47%
NaOMe
MeOH, dioxane
H
quant.
Syntheses of oligosaccharides 3-6: Glycosylation of known disaccharide acceptor 12 [3] with the
known D-fucopyranosyl donor 13 [6] in the presence of N-iodosuccinimide (NIS), trifluoro-
methanesulfonic acid (TfOH) [17] and MS4 Å in dichloromethane provided the desired α-glycoside 14
in 88% yield with complete α-stereoselectivity. The newly formed α-glycosidic linkage was confirmed
by 1H-NMR spectroscopy. The anomeric proton of the fucose moiety in 14 appeared at 4.85 ppm as a
doublet with a homonuclear proton-proton coupling constant of 3.7 Hz (H-1 of Fuc, δ = 4.85 ppm,
JH1,H2 = 3.7 Hz). Deprotection of the Troc group in 14 was achieved with Zn in a mixture containing
acetic anhydride and acetic acid, followed by catalytic hydrogenolysis over 10% Pd-C in MeOH and

Molecules 2011, 16
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acetylation to provide 15. Zemplén deacetylation and purification by column chromatography on
Sephadex LH-20 produced trisaccharide 3 quantitatively (Scheme 2).
Scheme 2. Synthesis of oligosaccharide 3.
R3O
OR3
O
PhS
BnO
O
Me
R2O
O
BnO
OAc
R3O
OAc
O
O
13
NIS, TfOH,
OBn
O
O
BnO
OR3
TMS
NHR1
Me
O
AcO
O
O
O
HO
R2O
TMS
OAc
NHTroc
MS 4 , CH2Cl2
R2O
2
OR
12
R1
R2
R3
14 Troc Bn Ac
15 Ac Ac Ac
Ac
88%
46%
1) Zn/AcOH, Ac2O 2) Pd-C/H2, MeOH
3) Ac2O, Pyr.
NaOMe, MeOH
3
H
H
quant.
The synthesis of disaccharides 4 and 5 and trisaccharide 6 is outlined in Schemes 3-5. Glycosylation
of known glycosyl acceptors 16, 19 [6] and 22 [18] with the D-fucopyranosyl donor 13 in the presence
of NIS, TfOH and MS4 Å in dichloromethane gave the desired α-glycosides 17 (68%), 20 (78%) and
the trisaccharide 23 (42%) with complete α-steroselectivity, respectively. The newly formed α-
1
glycosidic linkage was confirmed by H-NMR spectroscopy. The Troc-protecting group of 17 was
converted into an acetamido group by reduction with Zn-AcOH followed by debenzylidenation and
debenzylation with catalytic hydrogenolysis over 10% Pd/C in MeOH-AcOH and acetylation to afford
18 in 44% yield. Finally, standard deacetylation and purification by column chromatography on
Sephadex LH-20 furnished disaccharide 4 in 86% yield (Scheme 3).
Scheme 3. Synthesis of oligosaccharide 4.
Me
PhS
BnO
O
BnO
OBn
R2O
R3O
13
NIS, TfOH,
O
O
O
HO
O
Ph
O
O
TMS
NHR1
Me
O
MS 4 , CH2Cl2,
O
TMS
NHTroc
R4O
16
R4O
OR4
R1
R2 R3
PhCH
R4
Bn 68%
17 Troc
1) Zn/AcOH, Ac2O 2) Pd-C/H2, MeOH, AcOH
3) Ac2O, Pyr.
18
4
Ac
Ac
Ac
Ac Ac 44%
NaOMe, MeOH
H
H
H
86%
Disaccharide 5 was synthesized from the disaccharide 20 in a six steps deblocking/blocking
procedure (Scheme 4). At first, the chloroacetyl protecting group in 20 was deblocked with thiourea in
an ethanol/pyridine solvent mixture before conversion of the Troc group into an acetamido group using

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641
standard conditions. Debenzylation using catalytic hydrogenolysis followed by acetylation provided
protected disaccharide 21 in 45% yield which was deprotected using standard conditions to provide
disaccharide 5 (Scheme 4).
Scheme 4. Synthesis of oligosaccharide 5
OR3
PhS
BnO
Me
O
R3O
R3O
R3O
BnO
OBn
Me
13
NIS, TfOH,
O
BnO
O
HO
O
O
ClAcO
O
TMS
O
MS 4 , CH2Cl2,
R2O
NHTroc
TMS
R3
19
NHR1
R1
R2
20
21
5
Troc ClAc Bn 78%
1) thiourea, EtOH/Pyr. 2) Zn/AcOH
3) Pyr., Ac2O 4) Pd-C/H2, MeOH 5) Pyr., Ac2O
Ac
Ac
Ac
H
Ac 45%
quant.
NaOMe, MeOH
H
The remaining trisaccharide 6 was prepared from protected trisaccharide 23 in a five steps
procedure. Initially, the phthalimido-protecting group of 23 was removed using hydrazine
monohydrate in ethanol followed by standard acetylation, debenzylation by catalytic hydrogenation
over 10% Pd-C in MeOH-THF and acetylation to provide peracetylated trisaccharide 24 in 47% yield.
Finally, standard deacetylation and purification by column chromatography on Sephadex LH-20
provided disaccharide 6 (Scheme 5). Oligosaccharide 2 was prepared according to a procedure
previously reported by us [6].
Scheme 5. Synthesis of oligosaccharide 6
OR2
OR2
PhS
BnO
Me
O
R2O
b
BnO
O
Me
OR2
OBn
13
NIS, TfOH,
BnO
O
O
HO
O
O
HO
O
O
TMS
NPhth
TMS
R1
MS 4 , CH2Cl2
22
a
Me
OR2
R2
O
R2O
R2O
R1
23 NPhth Bn 42%
1) NH2NH2 įH2O, EtOH 2) Ac2O, Pyr.
3) Pd-C/H2, MeOH-THF 4) Ac2O, Pyr.
24 NHAc
6 NHAc
Ac 47%
93%
NaOMe, MeOH
H

Molecules 2011, 16
642
3.2. Inhibitory Effects of Synthetic Compounds on NO Production
The synthetic compounds were evaluated for their ability to inhibit nitric oxide (NO) production by
LPS-induced macrophage-like J774.1 cells [19] (Figure 2). NO, a short living mediator is synthesized
by a family of enzymes termed NO-synthase. Two types of NOS are recognized: constitutive isoforms
and inducible isoforms (iNOS). iNOS is regulated by inflammatory mediators (LPS, cytokines) and the
excessive production of NO by iNOS has been implicated in the pathogenesis of the inflammatory
response [14]. The glycolipid 1 showed comparable NO inhibitory activity in high concentration (100
μM) to NG-monomethyl-L-arginine (L-NMMA) used as the positive control. Related compounds
having the common D-Fucα1-3GlcNAc structure (i.e. 2, 3 and 4) also showed significant inhibitory
activity resulting in a 20% reduction of NO release at 50 μM and 100 μM concentrations. However,
very little or no inhibition of NO release were seen at these concentrations for disaccharide 5 and
trisaccharide 6 bearing an unnatural D-Fucα1-4GlcNAc linkage. Interestingly, glycosphingolipid 1
showed stronger activity than 2, suggesting that the ceramide-based aglycon contributes to the
inhibition of NO release more efficiently than a 2-(trimethylsilyl) ethyl-based aglycon. Moreover,
commercial ceramide 7 showed inhibitory activity at higher concentration (100 μM). However, the
activity of ceramide is strongly enhanced by glycosylation to the trisaccharide β-D-GalNAcp(1→4)[α-
D-Fucp(1→3)]-β-D-GlcNAc indicating that both the trisaccharide and ceramide-based aglycon portion
of the glycosphingolipid contribute to the inhibition of cellular nitric oxide release.
Figure 2. Inhibitory effects on NO production in LPS-activated J774.1 cells of compounds
1-7. Each data represents the mean ± SD for quadruplet experimentals. P: Positive control
(L-NMMA), 50 μM.

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3. Experimental
3.1. General
Optical rotations were measured with a Jasco P-1020 digital polarimeter. 1H- and 13C-NMR spectra
were recorded with JMN A500 and ECP 600 FT NMR spectrometers with Me4Si as the internal
standard for solutions in CDCl3 and CD3OD. MALDI-TOFMS was recorded on an Applied
Biosystems Voyager DE RP mass spectrometer. High-resolution mass spectra were recorded on a
JEOL JMS-700 under FAB conditions. TLC was performed on Silica Gel 60 F254 (E. Merck) with
detection by quenching of UV fluorescence and by charring with 10% H2SO4. Column
chromatography was carried out on Silica Gel 60 (E. Merck). The compounds 3,4,6-Tri-O-acetyl-2-
deoxy-2-(2,2,2-trichloroethoxycarbonylamino)-β-D-galactopyranosyl-(1→4)-[2,3,4-tri-O-acetyl-α-D-
fucopyranosyl-(1→3)]- 6-O-acetyl-2-deoxy-2-(2,2,2-trichloroethoxycarbonylamino)-D-glucopyranosyl
trichloroacetimidate (8) [6], 2-(trimethylsilyl)ethyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl-
(1→4)-6-O-benzyl-2-deoxy-2-(2,2,2-trichloroethoxycarbonylamino)-β-D-glucopyranoside (12) [3],
phenyl 2,3,4-tri-O-benzyl-1-thio-β-D-fucopyranoside (13) [6], 2-(Trimethylsilyl)ethyl 4,6-O-benzyl-
idene-2-deoxy-2-(2,2,2-trichloroethoxycarbonylamino)-β-D-glucopyranoside (16) [6], 2-(trimethyl-
silyl)ethyl 6-O-benzyl-3-O-chloroacetyl-2-deoxy-2-(2,2,2-trichloroethoxycarbonylamino)-β-D-gluco-
pyranoside (19) [6] and 2-(trimethylsilyl)ethyl 6-O-benzyl-2-deoxy-2-phthalimide-β-D-gluco-
pyranoside (22) [18] were prepared as reported. Benzylceramide 9 was prepared by the conventional
four-steps procedure [15] from phytosphingosine, which was purchased from Degussa (The
Netherlands).
3,4,6-Tri-O-acetyl-2-deoxy-2-(2,2,2-trichloroethoxycarbonylamino)-β-D-galactopyranosyl-(1→4)-
[2,3,4-tri-O-acetyl-α-D-fucopyranosyl-(1→3)]-6-O-acetyl-2-deoxy-2-(2,2,2-trichloroethoxycarbonyl-
amino)-β-D-glucopyranosyl-(1→1)-(2S,3S,4R)-3,4-di-O-benzyl-2-hexadecanamido-octadecane-3,4-di-
ol (10). Four Å molecular sieves (250 mg) were added to a solution of 8 (21 mg, 16.5 μmol) and
(2S,3S,4R)-3-O-benzoyl-2-hexadecanamido-4-octa-decene-1,3-diol 9 (24 mg, 30.0 μmol) in dry
CH2Cl2 (0.5 mL) and the mixture was stirred for 16 h at room temperature, then cooled to 0 °C.
TMSOTf (3 μL, 0.01 mmol) was added, and the mixture was stirred for 1 h at 0 °C, then neutralized
with Et3N. The solids were filtered off and washed with CHCl3. The combined filtrate and washings
were successively washed with water, dried (MgSO4), and concentrated. The product was purified by
23
silica gel column chromatography using 3:1 toluene-EtOAc as eluent to give 10 (10 mg, 33%). [α]D
= +18.2°(c 1.0, CHCl3); 1H-NMR (500 MHz, CDCl3): δ 4.94 (d, 1H, J1,2=3.7Hz, H-1 of fuc), 4.50 (br.
d, 1H, H-1 of GlcNAc), 4.34 (br. s, 1H, H-1, of GalNAc). MALDI-TOFMS: Calcd for
C86H129Cl6N3O27Na [M+Na]+: m/z 1868.7 Found: 1869.4.
2-Acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-D-galactopyranosyl-(1→4)-[2,3,4-tri-O-acetyl-α-D-fuco-
pyranosyl-(1→3)]-2-acetamido-6-O-acetyl-2-deoxy-β-D-glucopyranosyl-(1→1)-(2S,3S,4R)-hexa-
decanamido-octadecane-3,4-di-ol (11). To a solution of 10 (31mg, 16.8 μmol) in acetic anhydride (2
mL) and AcOH (2 mL) was added zinc powder (100 mg). The reaction mixture was stirred for 16 h at
room temperature. After completion of the reaction, the solids were filtered off and the filtrate was

Molecules 2011, 16
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concentrated with toluene. The solution of the product and Pd/C (10%, 100 mg) in 1:1 MeOH/THF
(2.0 mL) was stirred for 16 h at room temperature under H2, then filtered and concentrated. The
product was purified by silica gel column chromatography using 2:1 toluene acetone as eluent to give
11 as an amorphous powder (11 mg, 47%). [α]D23 = +5.3°(c 0.7, CHCl3); 1H-NMR (500 MHz, CDCl3):
δ 5.15 (d, 1H, J1,2=3.7Hz, H-1 of fuc), 4.33 (br. d, 1H, H-1 of GlcNAc), 4.30 (br. s, 1H, H-1, of
GalNAc). MALDI-TOFMS: Calcd for C70H119N3O25Na [M+Na]+: m/z 1424.8 Found: 1424.5.
2-Acetamido-2-deoxy-β-D-galactopyranosyl-(1→4)-[α-D-fucopyranosyl-(1→3)]-2-acetamido-2-
deoxy-β-D-glucopyranosyl-(1→1)-(2S,3S,4R)–hexadecanamido-octadecane-3,4-di-ol (1). To
a
solution of 11 (11 mg, 7.8 μmol) in MeOH (2 mL) was added dioxane (2 mL) and NaOMe (25 mg) at
40 °C. The mixture was stirred for 2 h and then neutralized with Amberlite IR 120 [H+]. The mixture
was filtered and concentrated. The product was purified by Sephadex LH-20 column chromatography
in 1:1 CHCl3-MeOH to give 1 as white solid (10 mg, quant.). [α]D25 +17.0 (c=0.06, 1:1 CHCl3-MeOH).
1H-NMR (500 MHz, 1:1 CDCl3-CD3OD): δ 5.13 (d, 1H, J=3.7Hz, H-1 of Fuc), 4.62 (d, 1H, J =8.3Hz,
H-1 of GlcNAc), 4.32 (d, 1H, d, 1H, J =8.0Hz, H-1 of GalNAc). MALDI-TOFMS: Calcd for
C56H105N3O18Na: m/z 1130.7 Found: 1130.4 [M+Na]+. HR-FABMS: Calcd for C56H105N3O18Na: m/z
1130.7291. Found m/z 1130.7257 [M+Na]+.
2-(Trimethylsilyl)ethyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl-(1→4)-[2,3,4-tri-O-benzyl-α-D-
fucopyranosyl-(1→3)]-6-O-benzyl-2-deoxy-2-(2,2,2-trichloroethoxy-
carbonylamino)-β-D-gluco-
pyranoside (14). To a solution of 12 (99 mg, 0.11 mmol) and 13 (89 mg, 0.17 mmol) in dry CH2Cl2
(1.5 mL) was added powdered MS 4Å (200 mg), and the mixture was stirred for 2 h at room
temperature, then cooled to -60 °C. NIS (57 mg, 0.03 mmol) and TfOH (1.5 μL, 0.01 mmol) were
added to the mixture, which was stirred for 3 h at -60 °C, then neutralized with Et3N. The solids were
filtered off and washed with CHCl3. The combined filtrate and washings were successively washed
with aq Na2S2O3 and water, dried (MgSO4), and concentrated. The product was purified by silica gel
column chromatography using 3:1 hexane-EtOAc as eluent to give 14 (128 mg, 88%). [α]D24 +20.4 (c
0.7, CHCl3); 1H-NMR (500 MHz, CDCl3): δ 7.28–7.14 (m, 20H, 4Ph), 5.74 (d, 1H, NH), 5.26 (d, 1H,
H-4 of Gal), 5.11 (t, 1H, H-2 of Gal), 4.92–4.84 (m, 2H, H-3 of Gal, benzylmethylene), 4.85 (d, 1H,
J1,2=3.7 Hz, H-1 of Fuc), 4.75–4.54 (m, 7H, benzylmethylene x 5, CH2CCl3), 4.40 (d, 1H, J1,2=7.3 Hz,
H-1 of GlcNAc), 4.38 (d, 1H, J1,2=7.9 Hz, H-1 of Gal), 4.27 (dd, 2H, benzylmethylene x 2), 4.06–4.02
(m, 2H, H-6a of GlcNAc, H-5 of Fuc), 3.97–3.75 (m, 9H, H-2, H-3, H-6b of GlcNAc, H-5, H-6 of Gal,
H-2, H-3 of Fuc, CH2CH2Si(CH3)3), 3.66–3.62 (m, 3H, H-4, H-5 of GlcNAc, H-4 of Fuc), 3.38–3.32
(m, 1H, CH2CH2Si(CH3)3), 2.07–1.87 (m, 12H, CH3CO x 4), 1.09 (d, 3H, H-6 of Fuc), 0.88–0.73 (m,
13
2H, CH2CH2Si(CH3)3), –0.09 (s, 9H, Si(CH3)3); C-NMR (125 MHz, CDCl3): δ 170.2, 170.0, 154.2,
139.0, 138.9, 138.7, 138.3, 128.4, 128.23, 128.20, 127.8, 127.74, 127.65, 127.59, 127.5, 127.3, 100.2
(C-1 of GlcNAc), 100.1 (C-1 of Gal), 99.3 (C-1 of Fuc), 95.8, 79.5, 77.8, 76.4, 76.3, 75.6, 74.8, 74.6,
74.1, 73.5, 73.2, 72.9, 71.0, 70.4, 69.0, 67.4, 66.8, 66.7, 61.1, 53.6, 29.7, 20.8, 20.62, 20.57, 18.2, 16.5,
–1.4 (Si(CH3)3); MALDI-TOFMS: Calcd for C62H78Cl3NO20SiNa: m/z 1312.4 Found: 1312.9
[M+Na]+.

Molecules 2011, 16
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2-(Trimethylsilyl)ethyl
2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl-(1→4)-[2,3,4-tri-O-acetyl-α-D-
fucopyranosyl-(1→3)]-6-O-acetyl-2-acetamido-2-deoxy-β-D-glucopyranoside (15). To a solution of 14
(107 mg, 0.08 mmol) in acetic anhydride (6 mL) and AcOH (6 mL) was added zinc powder (150 mg).
The reaction mixture was stirred for 12 h at 40 °C. After completion of the reaction, the solids were
filtered off and the filtrate was concentrated with toluene. The solution of the product and Pd/C (10%,
100 mg) in MeOH (2.0 mL) was stirred for 16 h at room temperature under H2, then filtered and
concentrated. The residue was acetylated with acetic anhydride (2 mL) in pyridine (3 mL) for 16 h at
room temperature. The reaction mixture was poured into ice-water and extracted with CHCl3. The
extract was washed sequentially with 5% HCl, aq NaHCO3 and water, dried (MgSO4), and
concentrated. The product was purified by silica gel column chromatography using 5:1 toluene-acetone
24
1
as eluent to give 15 (37 mg, 46%) as an amorphous powder. [α]D +11.8 (c 0.7, CHCl3); H-NMR
(500 MHz, CDCl3): δ 6.47 (d, 1H, NH), 5.36 (d, 1H, H-4 of Gal), 5.23–5.21 (m, 2H, H-2 of Gal, H-4
of Fuc), 5.10–5.00 (m, 3H, H-3 of Gal, H-2, H-3 of Fuc), 5.08 (d, 1H, J1,2=3.7 Hz, H-1 of Fuc), 4.57
(dd, 1H, H-6a of Gal), 4.52 (d, 1H, J1,2=7.9 Hz, H-1 of Gal),4.42 (dd, 1H, H-5 of Fuc), 4.37 (d, 1H,
J1,2=7.9 Hz, H-1 of GlcNAc), 4.33 (dd, 1H, H-6b of Gal), 4.24 (br d, 1H, H-2 of GlcNAc), 4.09 (d, 2H,
H-6 of GlcNAc), 3.93–3.85 (m, 4H, H-3, H-4 of GlcNAc, H-5 of Gal, CH2CH2Si(CH3)3), 3.69(s, 1H,
H-5 of GlcNAc), 2.18–1.81 (m, 27H, CH3CO x 9), 1.13 (d, 3H, H-6 of Fuc), 0.98–0.80 (m, 2H,
CH2CH2Si(CH3)3), –0.02 (s, 9H, Si(CH3)3); 13C-NMR (125 MHz, CDCl3): δ 170.9, 170.7, 170.5,
170.3, 170.2, 170.0, 169.9, 169.5, 99.5 (C-1 of Gal), 99.4 (C-1 of GlcNAc), 97.3 (C-1 of Fuc), 73.4,
72.5, 71.8, 71.3, 71.2, 69.9, 68.8, 68.2, 67.9, 66.7, 66.3, 65.7, 64.9, 61.0, 48.5, 22.9, 21.0, 20.9, 20.7,
20.6, 20.52, 20.47, 17.9, 15.7, –1.5 (Si(CH3)3); MALDI-TOFMS: Calcd for C41H63NO23SiNa: m/z
988.3 Found: 988.4 [M+Na]+.
2-(Trimethylsilyl)ethyl
β-D-galactopyranosyl-(1→4)-[α-D-fucopyranosyl-(1→3)]-2-acetamido-2-
deoxy-β-D-glucopyranoside (3). To a solution of 15 (36 mg, 0.04 mmol) in MeOH (5 mL) NaOMe (25
mg) was added at 40 °C. The mixture was stirred for 2 h and then neutralized with Amberlite IR 120
[H+]. The mixture was filtered and concentrated. The product was purified by Sephadex LH-20 column
24
chromatography in 1 : 1 CHCl3-MeOH to give 5 as white solid (24 mg, quant.). [α]D +14.4 (c 0.3,
CH3OH); 1H-NMR (500 MHz, CD3OD): δ 5.12 (d, 1H, J1,2=4.3 Hz, H-1 of Fuc), 4.42 (d, 1H, J1,2=7.9
13
Hz, H-1 of GlcNAc), 4.32 (d, 1H, J1,2=7.9 Hz, H-1 of Gal); C=NMR (125 MHz, CD3OD): δ 173.4,
104.1 (C-1 of GlcNAc), 102.2 (C-1 of Gal), 102.1 (C-1 of Fuc), 74.8, 73.6, 72.7, 71.5, 70.90, 70.86,
68.5, 67.9, 62.9, 61.1, 56.7, 30.7, 23.5, 18.8, 16.8, –1.3 (Si(CH3)3); MALDI-TOFMS: Calcd for
C25H47NO15SiNa: m/z 652.3 Found: 652.6 [M+Na]+. HR-FABMS: Calcd for C25H47NO15SiNa: m/z
652.2613. Found m/z 652.2642 [M+Na]+.
2-(Trimethylsilyl)ethyl
2,3,4-tri-O-benzyl-α-D-fucopyranosyl-(1→3)-4,6-O-benzylidene-2-deoxy-2-
(2,2,2-trichloroethoxycarbonylamino)-β-D-glucopyranoside (17). To a solution of 16 (329 mg,
0.61 mmol) and 13 (479 mg, 0.91 mmol) in dry CH2Cl2 (1.5 mL) was added powdered 4Å MS
(800 mg), and the mixture was stirred for 2 h at room temperature, then cooled to -60 °C. NIS (307
mg, 1.37 mmol) and TfOH (16 μL, 0.18 mmol) were added to the mixture, which was stirred for 3 h at
-60 °C, then neutralized with Et3N. The solids were filtered off and washed with CHCl3. The combined
filtrate and washings were successively washed with aq Na2S2O3 and water, dried (MgSO4), and

Molecules 2011, 16
concentrated. The product was purified by silica gel column chromatography using 7:1 hexane-EtOAc
646
24
1
as eluent to give 17 (394 mg, 68%). [α]D +18.9 (c 2.4, CHCl3); H-NMR (500 MHz, CDCl3):
δ 7.41–6.89 (m, 20H, 4Ph), 5.49 (br s, 1H, H-1 of Fuc), 5.31 (s, 1H, OCHPh), 5.14 (br s, 1H, NH),
4.86–4.75 (m, 3H, benzylmethylene x 2, CH2CCl3), 4.64 (d, 1H, J1,2=8.6 Hz, H-1 of GlcNAc), 4.60 (d,
1H, CH2CCl3), 4.48 (t, 2H, benzylmethylene x 2), 4.36–4.29 (m, 2H, benzylmethylene x 2), 4.25–4.18
(m, 2H, H-3, H-6a of GlcNAc), 3.96–3.85 (m, 3H, CH2CH2Si(CH3)3, H-2, H-5 of Fuc), 3.81–3.67 (m,
3H, H-4, H-6b of GlcNAc, H-3 of Fuc), 3.52–3.40 (m, 4H, CH2CH2Si(CH3)3, H-2, H-5 of GlcNAc, H-
4 of Fuc), 1.01 (d, 3H, H-6 of Fuc), 0.94–0.79 (m, 2H, CH2CH2Si(CH3)3), –0.07 (s, 9H, Si(CH3)3);
13C-NMR (125 MHz, CDCl3): δ 153.7, 138.8, 138.4, 138.2, 136.9, 129.3, 128.5, 128.34, 128.27, 128.1,
128.0, 127.5, 127.3, 127.1, 126.2, 101.6, 100.8 (C-1 of GlcNAc), 97.0 (C-1 of Fuc), 95.4, 82.5, 78.6,
75.1, 74.8, 74.4, 73.6, 73.3, 71.4, 68.7, 67.6, 67.0, 65.8, 57.0, 29.6, 18.2, 16.7, –1.5 (Si(CH3)3);
MALDI-TOFMS: Calcd for C48H58Cl3NO11SiNa [M+Na]+: m/z 980.3. Found: 980.1.
2-(Trimethylsilyl)ethyl 2,3,4-tri-O-acetyl-α-D-fucopyranosyl-(1→3)-2-acetamido-4,6-di-O-acetyl-2-
deoxy-β-D-glucopyranoside (18). To a solution of 17 (113 mg, 0.12 mmol) in acetic anhydride (7 mL)
and AcOH (7 mL) was added zinc powder (150 mg). The reaction mixture was stirred for 12 h at
40 °C. After completion of the reaction, the solids were filtrered off and the filtrate was concentrated
with toluene. The solution of the product and Pd/C (10%, 150 mg) in 3:1 MeOH-AcOH (2.0 mL) was
stirred for 12 h at room temperature under H2, then filtered and concentrated. The residue was
acetylated with acetic anhydride (6 mL) in pyridine (10 mL) for 12 h at room temperature. The
reaction mixture was poured into ice-water and extracted with CHCl3. The extract was washed
sequentially with 5% HCl, aq NaHCO3 and water, dried (MgSO4), and concentrated. The product was
purified by silica gel column chromatography using 5:1 toluene-acetone as eluent to give 18 (35 mg,
24
1
44%)as an amorphous powder. [α]D +74.9 (c 0.3, CHCl3); H-NMR (500 MHz, CDCl3): δ 5.85 (d,
1H, NH), 5.25 (d, 1H, J1,2=3.7 Hz, H-1 of Fuc), 5.26–5.23 (m, 2H, H-3, H-4 of Fuc), 5.08 (dd, 1H, H-2
of Fuc), 5.06 (d, 1H, J1,2=7.9 Hz H-1 of GlcNAc), 4.97 (dd, 1H, H-4 of GlcNAc), 4.67 (t, 1H, H-3 of
GlcNAc), 4.25 (dd, 1H, H-5 of Fuc), 4.14 (dd, 1H, H-6a of GlcNAc), 3.99 (dd, 1H, H-6b of GlcNAc),
3.92–3.87 (m, 1H, CH2CH2Si(CH3)3), 3.61–3.51 (m, 2H, H-5 of GlcNAc, CH2CH2Si(CH3)3),
3.09–3.04 (m, 1H, H-2 of GlcNAc), 1.08 (d, 3H, H-6 of Fuc), 0.96–0.83 (m, 2H, CH2CH2Si(CH3)3), –
0.02 (s, 9H, Si(CH3)3); 13C-NMR (125 MHz, CDCl3): δ 170.7, 170.5, 98.1 (C-1 of GlcNAc), 95.8 (C-1
of Fuc), 73.7, 72.3, 71.5, 71.0, 67.6, 67.4, 67.2, 64.9, 62.5, 58.1, 23.8, 20.9, 20.8, 20.6, 18.1, 16.1 –1.4
(Si(CH3)3); MALDI-TOFMS: Calcd for C29H47NO15SiNa [M+Na]+: m/z 700.3 Found: 700.5.
2-(Trimethylsilyl)ethyl α-D-fucopyranosyl-(1→3)-2-acetamido-2-deoxy-β-D-glucopyranoside (4).
Compound 4 was prepared from 18 (24 mg, 0.035 mmol) by the same method described for
preparation of 3. The product was purified by Sephadex LH-20 column chromatography in 1:1
24
1
CHCl3-MeOH to give 4 as white solid (14 mg, 86%). [α]D +52.9 (c 0.1, CH3OH); H-NMR (500
MHz, CD3OD): δ 4.93 (d, 1H, J1,2=3.1 Hz, H-1 of Fuc), 4.30 (d, 1H, J1,2=8.5 Hz H-1 of GlcNAc); 13C-
NMR (125 MHz, CD3OD): δ 173.3, 103.6 (C-1 of GlcNAc), 102.2 (C-1 of Fuc), 86.3, 79.5, 77.4, 73.7,
72.5, 71.6, 70.8, 68.5, 67.9, 62.6, 60.2, 55.9, 30.7, 23.4, 18.8, 16.9, –1.26 (Si(CH3)3); MALDI-
TOFMS: Calcd for C19H37NO10SiNa: m/z 490.2 Found: 490.6 [M+Na]+. HR-FABMS: Calcd for
C19H37NO10SiNa: m/z 490.2084. Found m/z 490.2072 [M+Na]+.

Molecules 2011, 16
647
2-(Trimethylsilyl)ethyl 2,3,4-tri-O-benzyl-α-D-fucopyranosyl-(1→4)-6-O-benzyl-3-O-chloroacetyl-2-
deoxy-2-(2,2,2-trichloroethoxycarbonylamino)-β-D-glucopyranoside (20). Compound 20 was prepared
from 19 (226 mg, 0.36 mmol) and 13 (383 mg, 0.73 mmol) by the same method described for
preparation of 17. The product was purified by silica gel column chromatography using10:1 hexane-
EtOAc as eluent to give 20 as syrup (296 mg, 78%). [α]D24 +11.4 (c 4.0, CHCl3); 1H-NMR (500 MHz,
CDCl3): δ 7.37–7.10 (m, 20H, 4 Ph), 5.49 (d, 1H, NH), 5.22 (t, 1H, H-3 of GlcNAc), 4.89 (d, 1H,
benzylmethylene), 4.87 (d, 1H, J1,2=3.7 Hz, H-1 of Fuc), 4.76–4.53 (m, 9H, H-4 of Fuc,
benzylmethylene x 6, CH2CCl3), 4.50 (d, 1H, J1,2=7.3 Hz, H-1 of GlcNAc), 4.45 (d, 1H,
benzylmethylene), 3.92–3.79 (m, 5H, H-4, 6a of GlcNAc, H-2, 5 of Fuc, CH2CH2Si(CH3)3), 3.75–3.62
(m, 5H, H-2, H-6b of GlcNAc, H-3 of Fuc, ClCH2CO), 3.51–3.46 (m, 2H, CH2CH2Si(CH3)3, H-5 of
GlcNAc), 0.97 (d, 3H, H-6 of Fuc), 0.98–0.83 (m, 2H, CH2CH2Si(CH3)3), –0.09 (s, 9H, Si(CH3)3);
13C-NMR (125 MHz, CDCl3): δ 167.1, 154.1, 138.5, 138.2, 138.1, 128.6, 128.43, 128.36, 128.3, 128.1,
127.62, 127.55, 127.47, 127.37, 100.1(C-1 of Fuc), 98.8(C-1 of GlcNAc), 95.5, 78.9, 77.7, 75.6, 74.9,
74.8, 74.6, 74.2, 74.0, 73.3, 73.2, 68.7, 67.6, 67.1, 55.5, 40.8, 18.1, 16.6, –1.32 (Si(CH3)3); MALDI-
TOFMS: Calcd for C50H61Cl4NO12SiNa [M+Na]+: m/z 1058.3 Found: 1059.2.
2-(Trimethylsilyl)ethyl 2,3,4-tri-O-acetyl-α-D-fucopyranosyl-(1→4)-2-acetamido-3,6-di-O-acetyl-2-
deoxy-β-D-glucopyranoside (21). To a solution of 20 (296 mg, 0.29 mmol) in EtOH (2.5 mL) was
added pyridine (1.5 mL) and thiourea (173 mg, 2.32 mmol). The reaction mixture was stirred for 6 h at
80 °C. The mixture was diluted with CHCl3, washed with aq 5%HCl, aq NaHCO3 and brine, dried
(MgSO4) and concentrated. The solution of the residue in AcOH (2 mL) was added zinc powder
(350 mg). The reaction mixture was stirred for 12 h at 60 °C. After completion of the reaction, the
solids were filtered off and the filtrate was concentrated with toluene. The residue was acetylated with
acetic anhydride (4 mL) in pyridine (7 mL). The reaction mixture was poured into ice-water and
extracted with CHCl3. The extract was washed sequentially with 5% HCl, aq. NaHCO3 and water,
dried (MgSO4), and concentrated. The solution of the product in MeOH (1.5 mL) and THF (0.5 mL)
was hydrogenolysed under hydrogen in the presence of 10% Pd/C (150 mg) for 16 h at room
temperature, then filtered and concentrated. The residue was acetylated with acetic anhydride (3 mL)
in pyridine (5 mL). The reaction mixture was poured into ice-water and extracted with CHCl3. The
extract was washed sequentially with 5% HCl, aq NaHCO3 and water, dried (MgSO4), and
concentrated. The product was purified by silica gel column chromatography using 5:1 toluene-acetone
24
1
as eluent to give 21 as an amorphous powder (86 mg, 45%). [α]D +49.6 (c 0.5, CHCl3); H-NMR
(500 MHz, CDCl3): δ 5.81 (d, 1H, NH), 5.34 (d, 1H, J1,2=3.7 Hz, H-1 of Fuc), 5.22–5.05 (m, 4H, H-3
of GlCNAc, H-2,H-3, H-4 of Fuc), 4.58 (d, 1H, J1,2=7.9 Hz H-1 of GlcNAc), 4.46 (dd, 1H, H-6a of
GlcNAc), 4.08–4.01 (m, 2H, H-6b of GlcNAc, H-5 of Fuc), 3.95 (t, 1H, H-4 of GlcNAc), 3.88–3.83
(m, 1H, CH2CH2Si(CH3)3), 3.78 (dd, 1H, H-2 of GlcNAc), 3.61–3.57 (m, 1H, H-5 of GlcNAc),
3.53–3.48 (m, 1H, CH2CH2Si(CH3)3), 2.11–1.82 (m, 18H, CH3CO x 6), 1.04 (d, 3H, H-6 of Fuc),
0.91–0.78 (m, 2H, CH2CH2Si(CH3)3), –0.07 (s, 9H, Si(CH3)3); 13C-NMR (125 MHz, CDCl3): δ 171.0,
170.7, 170.5, 170.3, 170.1, 170.0, 99.8 (C-1 of GlcNAc), 96.0 (C-1 of Fuc), 75.5, 72.1, 71.8, 70.9,
67.3, 67.2, 66.9, 65.5, 62.7, 54.6, 29.6, 23.1, 20.9, 20.8, 20.7, 20.6, 20.5, 17.8, 15.8, –1.5 (Si(CH3)3);
MALDI-TOFMS: Calcd for C29H47NO15SiNa [M+Na]+: m/z 700.3 Found: 700.9.

Molecules 2011, 16
648
2-(Trimethylsilyl)ethyl α-D-fucopyranosyl-(1→4)-2-acetamido-2-deoxy-β-D-glucopyranoside (5).
Compound 5 was prepared from 21 (86 mg, 0.13 mmol) by the same method described for preparation
of 3. The product was purified by Sephadex LH-20 column chromatography in 1:1 CHCl3-MeOH to
24
1
give 5 as white solid (61 mg, quant.). [α]D +33.9 (c 0.3, CH3OH); H-NMR (500 MHz, CD3OD): δ
4.98 (d, 1H, J1,2=3.7 Hz, H-1 of Fuc), 4.32 (d, 1H, J1,2=7.9 Hz H-1 of GlcNAc); 13C-NMR (125 MHz,
CD3OD): δ 173.5, 103.5 (C-1 of Fuc), 102.0 (C-1 of GlcNAc), 82.2, 76.9, 75.9, 73.5, 71.7, 70.6, 68.6,
67.9, 62.5, 56.8, 30.7, 23.0, 18.8, 16.7, –1.3 (Si(CH3)3); MALDI-TOFMS: Calcd for C19H37NO10SiNa:
m/z 490.2 Found: 491.0 [M+Na]+. HR-FABMS: Calcd for C19H37NO10SiNa: m/z 490.2084. Found m/z
490.2062 [M+Na]+.
2-(Trimethylsilyl)ethyl
2,3,4-tri-O-benzyl-α-D-fucopyranosyl-(1→3)-[2,3,4-tri-O-benzyl-α-D-fuco-
pyranosyl-(1→4)]-6-O-benzyl-2-deoxy-2-phthalimido-β-D-glucopyranoside (23). Compound 23 was
prepared from 22 (89 mg, 0.18 mmol) and 13 (757 mg, 1.44 mmol) by the same method described for
preparation of 14. The product was purified by silica gel column chromatography using 10:1 hexane-
24
1
EtOAc as eluent to give 23 as syrup (99 mg, 42%). [α]D +48.3 (c 1.2, CHCl3); H-NMR (600 MHz,
CDCl3): δ 7.90–7.26 (m, 39H, NPhth, 8 Ph), 6.20 (d, 1H, J1,2=3.6 Hz, H-1 of Fuc b), 5.19 (d, 2H,
J1,2=8.5 Hz H-1 of GlcNAc, J1,2=4.4 Hz H-1 of Fuc a), 5.07 (d, 1H, benzylmethylene), 5.01 (dd, 1H,
H-3 of GlcNAc), 4.93–4.88(m, 3H, benzylmethylene x 3), 4.79–4.66 (m, 8H, benzylmethylene x 8),
4.62–4.58 (m, 2H, benzylmethylene x 2), 4.41 (dd, 1H, H-2 of GlcNAc), 4.18–4.13 (m, 2H, H-4 of
GlcNAc, H-2 of Fuc b), 4.10 (dd, 1H,H-3 of Fuc a), 4.07–3.99 (m, 2H, CH2CH2Si(CH3)3, H-5 of Fuc
b), 3.97–3.84 (m, 6H, H-5, H-6 of GlcNAc, H-2, H-5 of Fuc a, H-3 of Fuc b), 3.63–3.57 (m, 2H, H-4
of Fuc b, CH2CH2Si(CH3)3), 3.47(br.d, 1H, H-4 of Fuc a), 1.19 (d, 3H, H-6 of Fuc b) 0.95 (d, 3H, H-6
of Fuc a,), 0.93–0.81 (m, 2H, CH2CH2Si(CH3)3), –0.01 (s, 9H, Si(CH3)3); 13C-NMR (150 MHz,
CDCl3): δ 139.1, 138.7, 138.52, 138.50, 138.3, 133.8, 128.3, 128.23, 128.18, 128.12, 128.05, 128.03,
127.93, 127.87, 127.7, 127.6, 127.5, 127.42, 127.37, 127.35, 127.28, 127.23, 127.1, 123.1, 169.3, 9,
97.6 (C-1 of GlcNAc), 97.5 (C-1 of Fuc a), 95.0 (C-1 of Fuc b), 78.8, 78.7, 78.4, 78.1, 78.0, 76.4, 76.3,
74.9, 74.6, 73.5, 73.3, 73.1, 73.0, 72.9, 72.8, 69.7, 67.7, 67.0, 66.7, 56.3, 29.7, 17.8, 16.6, 15.8, –1.5
(Si(CH3)3); MALDI-TOFMS: Calcd for C80H89NO15SiNa [M+Na]+: m/z 1354.6 Found: 1354.8.
2-(Trimethylsilyl)ethyl
2,3,4-tri-O-acetyl-α-D-fucopyranosyl-(1→3)-[2,3,4-tri-O-acetyl-α-D-fuco-
pyranosyl-(1→4)]- 2-acetamido-6-O-acetyl-2-deoxy-β-D-glucopyranoside (24). To a solution of 23
(60 mg, 0.05 mmol in EtOH (10 mL)) was added hydrazine monohydrate (3.3 mL, 0.07 mmol). The
reaction mixture was refluxed for 3 h, then concentrated. The residue was acetylated with Ac2O (3 mL)
in pyridine (5 mL). The mixture was poured into ice-water and extracted with CHCl3. The extract was
washed sequentially with 5% HCl, aq NaHCO3 and water, dried (MgSO4), and concentrated. The
solution of the product in MeOH (1 mL) and THF (1 mL) was hydrogenolysed under hydrogen in the
presence of 10% Pd/C (100 mg) for 15 h at room temperature, then filtered and concentrated. The
residue was acetylated with acetic anhydride (5 mL) in pyridine (7 mL). The reaction mixture was
poured into ice-water and extracted with CHCl3. The extract was washed sequentially with 5% HCl, aq
NaHCO3 and water, dried (MgSO4), and concentrated. The product was purified by silica gel column
chromatography using 9:1 toluene-acetone as eluent to give 24 as syrup (19 mg, 47%). [α]D24 +75.2 (c
0.5 CHCl3); 1H-NMR (500 MHz, CDCl3): δ 6.43 (d, 1H, NH), 5.28 (dd, 1H, H-3 of Fuc b), 5.23–5.18

Molecules 2011, 16
649
(m, 3H, H-3, H-4 of Fuc a, H-4 of Fuc b), 5.13 (d, 1H, J1,2=3.7 Hz, H-1 of Fuc b), 5.08–5.03 (m, 2H,
H-3 of GlcNAc, H-2 of Fuc b), 5.04 (d, 1H, J1,2=9.8 Hz H-1 of GlcNAc), 4.66 (d, 1H, J1,2=3.7 Hz H-1
of Fuc a), 4.52 (dd, 1H, H-6a of GlcNAc), 4.43–4.36 (m, 2H, H-6b of GlcNAc, H-5 of Fuc b),
4.13–4.08 (m, 2H, H-2 of GlcNAc, H-5 of Fuc a), 3.96 (t, 1H, H-5 of GlcNAc), 3.91–3.85 (m, 1H,
CH2CH2Si(CH3)3), 3.60–3.55 (m, 2H, H-4 of GlcNAc, H-2 of Fuc a), 3.46–3.40 (m, 1H,
CH2CH2Si(CH3)3), 2.13–1.87 (m, 21H, CH3CO) 1.10 (d, 3H, H-6 of Fuc b) 1.07 (d, 3H, H-6 of Fuc a),
0.95–0.80 (m, 2H, CH2CH2Si(CH3)3), –0.03 (s, 9H, Si(CH3)3); 13C-NMR (125 MHz, CDCl3): δ 170.5,
170.43, 170.36, 170.31, 169.9, 169.5, 169.3, 98.5 (C-1 of Fuc a), 98.2 (C-1 of Fuc b), 97.6 (C-1 of
GlcNAc), 76.9, 76.8, 76.7, 74.1, 74.0, 73.7, 73.4, 71.2, 70.9, 68.3, 68.00, 67.97, 67.3, 66.5, 65.8, 65.7,
64.5, 49.3, 29.7, 23.4, 21.4, 20.9, 20.7, 20.6, 18.0, 16.0, 15.8, 15.7, 14.1, –1.5 (Si(CH3)3); MALDI-
TOFMS: Calcd for C39H61NO21SiNa [M+Na]+: m/z 930.3 Found: 930.5.
2-(Trimethylsilyl)ethyl α-D-fucopyranosyl-(1→3)-[ α-D-fucopyranosyl-(1→4)]- 2-acetamido-2-deoxy-
β-D-glucopyranoside (6). Compound 6 was prepared from 24 (16 mg, 0.03 mmol) by the same method
described for preparation of 3. The product was purified by Sephadex LH-20 column chromatography
24
1
in 1:1 CHCl3-MeOH to give 6 as white solid (10 mg, 93%). [α]D +72.2 (c 0.2 CH3OH); H-NMR
(600 MHz, CD3OD): δ 4.97 (d, 1H, J1,2=3.9 Hz H-1 of Fuc b), 4.66 (d, 1H, J1,2=2.8 Hz H-1 of Fuc a),
4.38 (d, 1H, J1,2=6.1 Hz H-1 of GlcNAc), –0.09 (s, 9H, Si(CH3)3); 13C-NMR (150 MHz, CD3OD):
δ 172.8, 101.8 (C-1 of GlcNAc), 101.6 (C-1 of Fuc a), 100.1 (C-1 of Fuc b), 79.3, 78.3, 74.8, 73.7,
73.5, 71.4, 70.1, 69.8, 68.5, 68.3, 67.6, 63.1, 54.7, 27.0, 23.1, 18.9, 16.7, –1.3 (Si(CH3)3);
MALDI-TOFMS: Calcd for C25H47NO14SiNa: m/z 636.3 Found: 636.7 [M+Na]+. HR-FABMS: Calcd
for C25H47NO14SiNa: m/z 636.2664. Found m/z 636.2681 [M+Na]+.
3.2. Nitric Oxide Inhibitory Assay
J774.1 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM, GIBCO) and cultured
at 37°C in humidified 5% CO2/95% air. The cells were suspended in medium, plated on 96-well
culture plates (Falcon) at a density of 5.0 × 105 cells/mL/well, volume of 200 μL/well and allowed to
adhere for 24 h. Then, the medium was replaced with fresh medium, containing LPS (1 μg/mL) from
E. coli (Sigma) and test compounds dissolved in DMSO at various concentrations (13, 25, 50, 100 μM)
were incubated for 24 h. NO production was determined by measuring the accumulation of nitrite
(a stable metabolite of NO) in the culture supernatant using Griess reagent [20]. Briefly, 50 μL of the
supernatant from incubates were mixed with equal volume of Griess reagent (1% sulfanilamide and
0.1% N-1-naphthylenediamine dihydrochloride in 5% H3PO4) and were allowed to stand for 10
minutes at room temperature. Absorbance at 550 nm was measured using a MTP-810 Microplate
Reader (Corona Co.). The blank correction was carried out by subtracting the absorbance due to
medium from the absorbance reading of each well. The reaction percentage was calculated as follows:
% of control = [As/Ac] × 100, where As and Ac are absorbance of a run treated with LPS and a
sample, and that treated with LPS alone, respectively. In this assay, NG-monomethyl-L-arginine
(L-NMMA, IC50 32.0 μM), a non-selective nitric oxide synthase (NOS) inhibitor, was used as a
positive control [21].

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4. Conclusions
We have succeeded for the first time in carrying out the total syntheses of D-fucose-containing
glycosphingolipids found in invertebrate species. Both the presence of a D-Fucα1-3GlcNAc-linkage
and the ceramide aglycon portion resulted in a significant enhancement of their ability to inhibit NO
production by LPS-induced macrophage-like J774.1 cells. The prepared glycolipids are
easily-accessible target compounds in the field of carbohydrate chemistry and may serve as chemical
probes to explore glycosphingolipid-mediated anti-inflammatory processes in biology and medicine.
Acknowledgements
This work was supported by a Grant-in-Aid for Scientific Research (No. 19590011) from the
Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), and the (MEXT)
Open Research Center project. The authors are grateful to J. Hada for providing HR-FABMS data.
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