Enantioselective synthesis of possible diastereomers of heptadeca-1-ene-4,6-diyne-3,8,9,10-tetrol; Putative structure of a conjugated diyne natural product isolated from hydrocotyle leucocephala

Article abstract of DOI:10.1021/jo102201k

Enantioselective synthesis of possible diastereomers of heptadeca-1-ene-4,6-diyne-3,8,9,10-tetrol, a structure proposed for the natural product isolated from Hydrocotyle leucocephala is accomplished. The reported spectral data of the natural product did n

Full text of DOI:10.1021/jo102201k

ARTICLE
pubs.acs.org/joc
Enantioselective Synthesis of Possible Diastereomers of Heptadeca-
1-ene-4,6-diyne-3,8,9,10-tetrol; Putative Structure of a Conjugated
Diyne Natural Product Isolated from Hydrocotyle leucocephala
Kavirayani R. Prasad* and Bandita Swain
Department of Organic Chemistry, Indian Institute of Science, Bangalore 560012, India
S Supporting Information
b
ABSTRACT: Enantioselective synthesis of possible diastereomers of heptadeca-1-ene-4,6-diyne-
3,8,9,10-tetrol, a structure proposed for the natural product isolated from Hydrocotyle leucocephala
is accomplished. The reported spectral data of the natural product did not match those of any of the
isomers that were synthesized and established that the structure proposed for the natural product is
not correct and requires revision.
’ INTRODUCTION
Conjugated diyne containing natural products are an impor-
tant class of compounds possessing diverse biological activities.
With the isolation of conjugated diynes such as panaxytriol,
which was found to exhibit inhibitory activity against MK-1 cells
and to mitigate the growth of B16 melanoma cells, there has
been a surge of interest in the synthesis of conjugated diynes
and their analogues and derivatives with the aim of drug
development.1 Recently, Ramos et al. described the isolation of
a conjugated diyne tetrol 1, from the methanolic extract of
Hydrocotyle leucocephala, an aquatic plant native of Brazil.2
Structure of the diyne tetrol was proposed to be heptadeca-1-
ene-4,6-diyne-3,8,9,10-tetrol 1. Relative stereochemistry of the
contiguous triol unit was assigned to be [8R,9R,10R] based on
the method proposed by Kishi’s group3 for structurally related
alkane triols; however, the stereochemistry at the C3 carbon was
not assigned.
Recently, Ghosh and Pradhan disclosed the synthesis of both
3R and 3S isomers of the putative structure [8R,9R,10R] and
’ RESULTS AND DISCUSSION
found that the spectral data did not match with the published
data of the natural product.4 It was suggested that the natural
At the outset, we focused on the synthesis of [3R,8S,9S,10R]-
product could be one of the other six diastereomers. In an effort
to establish the relative as well as absolute structure of the natural
product, we undertook the synthesis of the remaining six possible
isomers 1c-h of heptadeca-1-ene-4,6-diyne-3,8,9,10-tetrol, and
we delineate our investigations in this article.
heptadeca-1-ene-4,6-diyne-tetrol 1c and [3S,8S,9S,10R] hepta-
deca-1-ene-4,6-diyne-tetrol 1d (Scheme 1). Synthesis of the
Received: November 4, 2010
Published: March 08, 2011
r
2011 American Chemical Society
2029
dx.doi.org/10.1021/jo102201k J. Org. Chem. 2011, 76, 2029–2039
|

The Journal of Organic Chemistry
ARTICLE
conjugated diyne unit is envisaged through a Cadiot-Chodkie-
wicz coupling of appropriately protected alkyne triol 3 with
either enantiomer of 3-silyloxy-pent-1-en-4-yne 2. Formation of
the alkyne 3 is anticipated from oxidation of alcohol 4 to the
aldehyde and subsequent alkynylation. Elaboration of the γ-
hydroxy amide 5 derived from the bis-dimethylamide 6 of tartaric
acid is planned for the synthesis of 4.
The synthetic sequence commenced with the addition of 1.5
equiv of n-heptylmagnesium bromide to diamide 65 and afforded
the γ-oxo-amide 7 in 73% yield (Scheme 2).6 Reduction of the
keto group in 7 with NaBH4/CeCl3 gave a nonseparable
diastereomeric mixture of alcohols in a 95:5 ratio, 5 being the
major diastereomer in 90% yield. Conversion of the amide 5 to
the methyl ester with trans disposition of the acetonide is
accomplished in a two-step process. Thus, treatment of 5 with
p-TSA in benzene at 50 °C resulted the lactone 8 in 80% yield
after chromatography. Reaction of the lactone with excess 2,2-
dimethoxypropane in the presence of p-TSA in dichloromethane
afforded 9 in 87% yield.7 Protection of the hydroxy group in 9 as
the silyl ether followed by reduction of the ester with LiBH4
furnished the primary alcohol 4 in 88% yield. Oxidation of 4 with
IBX resulted in the aldehyde, which was transformed into the
alkynol 11 using Corey-Fuchs8 protocol and silyl ether depro-
tection using TBAF. Bromination of 11 with NBS in the presence
of catalytic AgNO3 produced the bromoalkyne 3 in 90% yield.
Synthesis of (R)-3-silyloxypent-1-en-4-yne is accomplished
from the known alcohol 129 as depicted in Scheme 3. Alcohol 12
was converted to the corresponding iodide, which on reaction
with zinc dust afforded the allylic alcohol 13 in 87% yield. The
free secondary hydroxy group in 13 was protected as the tert-
butyldiphenylsilyl ether 14, which on DDQ-mediated debenzy-
lation afforded the primary alcohol 15 in 80% yield. Oxidation of
15 furnished the aldehyde, which on treatment with Ohira-
Bestmann reagent10 yielded (R)-3-silyloxypent-1-en-4-yne 2a
(27% for two steps). Similarly, employing the alcohol derived
from D-tartaric acid afforded (S)-3-silyloxypent-1-en-4-yne 2b.
Cadiot-Chodkiewicz coupling11 of the alkynes 3 and 2a
produced the conjugated diyne 16 in 45% yield (Scheme 4).12
Deprotection of the acetonide and the silyl group in 16 was
accomplished by treating the diyne with 4 N HCl in MeOH to
afford [3R,8S,9S,10R]-heptadeca-1-ene-4,6-diyne-tetrol 1c in
69% yield. In a similar way the [3S,8R,9R,10S]-heptadeca-1-
ene-4,6-diyne-tetrol 1d was prepared by employing 2b in the
Scheme 1. Retrosynthesis for [3R,8S,9S,10R]- and
[3S,8S,9S,10R]-Heptadeca-1-ene-4,6-diyne-tetrol
Scheme 3. Synthesis of (R)-3-Silyloxypent-1-en-4-yne 2a
Scheme 2. Synthesis of Alkyne Triol 3 from Tartaric Acid Diamide
2030
dx.doi.org/10.1021/jo102201k |J. Org. Chem. 2011, 76, 2029–2039

The Journal of Organic Chemistry
ARTICLE
Scheme 4. Synthesis of 3R- and 3S-[8S,9S,10R]-Heptadeca-
1-ene-4,6-diyne-tetrols 1c and 1d
Scheme 6. Synthesis of 3R- and 3S-[8R,9S,10R]-Heptadeca-
1-ene-4,6-diyne-tetrol 1e and 1f
Scheme 5. Retrosynthesis for [3R,8R,9S,10R]- and
[3S,8R,9S,10R]-Heptadeca-1-ene-4,6-diyne-tetrol
Scheme 7. Retrosynthesis for [3R,8R,9S,10S]- and
[3S,8R,9S,10S]-Heptadeca-1-ene-4,6-diyne-tetrol
reaction sequence. Unlike the natural product, compound 1d was
not soluble in CDCl3, and neither the spectral data of isomers 1c
and 1d nor the optical rotations were in agreement with those
reported for the natural product.
We then turned our attention to the synthesis of
[3R,8R,9S,10R]-heptadeca-1-ene-4,6-diyne-3,8,9,10-tetrol 1e
and [3S,8R,9S,10R]-heptadeca-1-ene-4,6-diyne-3,8,9,10-tetrol
1f. As depicted in Scheme 5, synthesis of the required alkyne
triol unit 17 can be envisaged by the elaboration of the R-hydroxy
ester 18, which in turn can be obtained by Mitsunobu inversion
of 9, the synthesis of which is described in Scheme 2 (vide supra).
Accordingly, Mitsunobu inversion13 of the hydroxy group in 9
afforded the epimeric ester 18 in good yield (Scheme 6).
Employing a synthetic sequence similar to that described for
the synthesis of 1c and 1d (vide supra), ester 18 was elaborated to
the alkyne triol 17 (see Supporting Information for experimental
procedures). Coupling of 17 with 2a afforded the conjugated
diyne 20, which on deprotection of the protecting groups
afforded the diyne tetrol 1e in 69% yield. Similarly, employing
2b in the synthetic sequence afforded the other diastereomer 1f.
Contrary to the natural product, 1e and 1f are not soluble in
CDCl3, and it turned out that the spectral and physical data of the
diastereomers 1e and 1f are also not in agreement with that
reported for the isolated compound.
Realizing the fact that the data of none of the six diastereomers
1a-1f match with that reported, synthesis of the remaining two
possible diastereomers 3R,8R,9S,10S (1g) and 3S,8R,9S,10S (1h)
was planned. For this purpose D-ribose was chosen as the
appropriate starting compound possessing the right stereochem-
istry required for the 8R,9S,10S alkyne triol unit (Scheme 7).
Wittig olefination of the lactol 23 with n-C6H13PPh3Br was
identified as the key reaction for the assembly of the vital triol unit.
Thus, D-ribose was converted to the known lactol 23 employ-
ing literature procedure (Scheme 8).14 Wittig olefination of 23
produced the olefin 2415 in 60% yield, which was hydrogenated
over Pd/C to yield the diol 22 in 96% yield. Reaction of 22 with
Bu2SnO followed by benzyl bromide resulted in regioselective
2031
dx.doi.org/10.1021/jo102201k |J. Org. Chem. 2011, 76, 2029–2039

The Journal of Organic Chemistry
ARTICLE
Scheme 8. Synthesis of the Alkynetriol 21 from D-Ribose
Scheme 9. Synthesis of 3R- and 3S-[8R,9S,10S]-Heptadeca-
1-ene-4,6-diyne-tetrol 1g and 1h
benzylation of the primary alcohol, which on treatment with
TBDMSCl/imidazole furnished the silyl ether 25 in good yield.
Hydrogentaion of 25 over Pd/C afforded the primary alcohol 26
in almost quantitative yield. Oxidation of alcohol 26 furnished
the corresponding aldehyde, which was transformed into the
alkyne 28 employing Corey-Fuchs protocol. TBAF-mediated
deprotection of the silyl ether yielded the alcohol 29, which on
bromination with NBS in the presence of catalytic amount of
silver nitrate produced the bromo alkyne 21 in 98% yield.
Following a sequence that is described for the synthesis of
conjugated diyne tetrols (vide supra) alkyne 21 was elaborated to
[3R,8R,9S,10S]-heptadeca-1-ene-4,6-diyne-tetrol (1g) and [3S,
8R,9S,10S]-heptadeca-1-ene-4,6-diyne-tetrol (1h) (Scheme 9)
(see Supporting Information for experimental details).
Neither the spectral data nor the physical data of the diaster-
eomers 1g-1h match with that reported for the natural product.
Spectral data and specific rotation of all eight possible diastere-
omers of heptadec-1-ene-4,6-diyne-3,8,9,10-tetrol are summar-
ized in Table 1 along with those reported for the natural product.
A perusal of the data clearly reveals that the spectral data of the
natural product did not match with any of the eight diastereo-
mers [for example, none of the diastereomers exhibited a dt
resonance at δ 4.27 in the 1H NMR as reported for the natural
product]. Hence it is obvious that the structure assigned for the
natural product is not correct and requires revision.
In conclusion, enantioselective synthesis of possible diastere-
omers of heptadeca-1-ene-4,6-diyne-3,8,9,10-tetrol, a structure
proposed for the natural product isolated from Hydrocotyle
leucocephala, is accomplished. The two alkyne precursors re-
quired for the construction of the diyne moiety are synthesized
from tartaric acid and D-ribose. The key diyne moiety is
assembled via copper-catalyzed Cadiot-Chodkiewicz coupling.
It was found that the NMR spectral data of the putative structure
assigned for the natural product did not match with any of the
diastereomers that were synthesized. This establishes that the
structure proposed for the natural product is wrong and requires
revision. Perhaps location of the triple bond and/or the four
hydroxy groups is in a different position on the heptadecene in
the natural product.
’ EXPERIMENTAL SECTION
Preparation of 7. In an oven-dried two-neck 100 mL, round-
bottom flask equipped with a magnetic stir bar, rubber septum, and
argon inlet was placed 6 (2 g, 8.2 mmol) dissolved in THF (20 mL). The
solution was cooled to -15 °C, and n-C7H15MgBr (12.3 mmol, 24.6 mL
of 0.5 M solution in THF) was added slowly and stirred for 0.5 h at the
same temperature. After the reaction was complete (indicated by TLC),
it was cautiously quenched by addition of an ice-cold solution of
saturated NH4Cl (20 mL). The reaction mixture was poured into water
(25 mL) and extracted with EtOAc (3 ꢀ 25 mL). The combined organic
layers were washed with brine (20 mL) and dried (Na2SO4). The
residue obtained after removal of the solvent was purified by silica gel
column chromatography with EtOAc/petroleum ether (1:4) to yield 7
2032
dx.doi.org/10.1021/jo102201k |J. Org. Chem. 2011, 76, 2029–2039

The Journal of Organic Chemistry
ARTICLE
Table 1. NMR Spectral Data for Diastereomers (1a-1h) of Heptadec-1-ene-4,6-diyne-3,8,9,10-tetrol
2033
dx.doi.org/10.1021/jo102201k |J. Org. Chem. 2011, 76, 2029–2039

The Journal of Organic Chemistry
ARTICLE
Table 1. Continued
a From ref 4. b In contrast to the reported data, these isomers are not soluble in CDCl3 and the spectra is recorded in CD3OD.
(1.79 g, 73%) as a colorless oil. Rf 0.5 (2:3 EtOAc/petroleum ether);
Preparation of 9. To a stirred solution of the lactone 8 (0.7 g, 3.25
mmol) in CH2Cl2 (10 mL) were added p-toluenesulphonic acid (62 mg,
0.32 mmol) and 2,2-dimethoxy propane (0.8 mL, 6.5 mmol). The
reaction mixture was stirred at room temperature for 1 h and after
completion of the reaction (TLC), K2CO3 (45 mg) was added and
stirred for 15 min. The reaction mixture was then passed through a pad
of Celite, and the Celite pad was washed with diethyl ether (20 mL).
Evaporation of solvent followed by silica gel column chromatography of
the resultant residue with petroleum ether/EtOAc (9:1) as eluent
afforded R-hydroxy ester 9 in 87% (0.81 g) yield. Rf 0.6 (1:4 EtOAc/
petroleum ether); [R]26D þ18.4 (c 3.00, CHCl3); IR (neat) 3491, 2987,
2930, 2857, 1749, 1253 cm-1; 1H NMR (400 MHz, CDCl3) δ 4.10-
4.08 (m, 2H), 3.87 (dd, 1H, J = 8.3, 1.2 Hz), 3.83 (s, 3H), 3.02 (d, 1H, J =
9.1 Hz), 1.65-1.45 (m, 3H), 1.42-1.23 (m, 15 H), 0.88 (t, 3H, J = 7
Hz); 13C NMR (100 MHz, CDCl3) δ 172.8, 109.0, 81.3, 76.3, 68.7, 52.6,
32.4, 31.6, 29.5, 29.0, 27.3, 26.4, 25.8, 22.5, 13.9; HRMS (m/z) [M þ
Na]þ calcd for C15H28O5, 311.1834; found, 311.1839.
[R]26 þ14.6 (c 3.50, CHCl3); IR (neat) 2930, 1716, 1653, 1374,
D
1058 cm-1; 1H NMR (400 MHz, CDCl3) δ 5.12 (d, 1H, J = 5.6 Hz),
4.79 (d, 1H, J = 5.6 Hz). 3.13 (s, 3H), 2.97 (s, 3H), 2.71-2.55 (m, 2H),
1.62-1.53 (m, 2H), 1.41 (s, 6H), 1.27 (brm, 8H), 0.85 (t, 3H, J = 6.7
Hz); 13C NMR (100 MHz, CDCl3) δ 209.4, 168.1, 112.0, 82.0, 74.8,
39.4, 36.9, 35.9, 31.5, 29.0, 28.9, 26.3, 26.0, 23.0, 22.5, 14.0; HRMS
(m/z) [M þ Na]þ calcd for C16H29NO4, 322.1994; found, 322.1990.
Preparation of 5. To a solution of 7 (1.6 g, 5.4 mmol) in MeOH
(25 mL) was added CeCl3 7H2O (4.02 g, 10.8 mmol), and the mixture
3
was stirred for 1 h. NaBH4 (0.41 g, 10.8 mmol) was then added
portionwise at -78 °C and stirred at the same temperature for 1 h.
After the reaction was complete (TLC), it was quenched by the addition
of water (1 mL). Most of the volatiles were removed under reduced
pressure, and the solid residue thus obtained was dissolved in 1 N HCl
(8 mL) and extracted with diethyl ether (3 ꢀ 10 mL). The combined
ethereal layers were washed with brine (10 mL), dried over Na2SO4, and
concentrated. The crude residue obtained was purified by silica gel
column chromatography with EtOAc/petroleum ether (3:7) to give the
alcohol 5 in 90% (1.45 g) yield as a colorless oil. Rf 0.5 (1:1 EtOAc/
petroleum ether); [R]26D -10.8 (c 2.30, CHCl3); IR (neat) 3447, 2986,
2930, 2857, 1651, 1380 cm-1; 1H NMR (300 MHz, CDCl3) δ 4.49-
4.48 (m, 2H), 3.53-3.50 (m, 1H), 3.07 (s, 3H), 2.89 (s, 3H), 1.99 (d,
1H, J = 9.3 Hz), 1.47-1.20 (m, 18H), 0.80 (t, 3H, J = 7.5 Hz) 13C NMR
(100 MHz, CDCl3) δ 168.8, 110.1, 80.1, 74.1, 69.9, 37.0, 35.9, 35.6, 34.7,
31.7, 29.4, 29.1, 26.7, 25.8, 22.6, 14.0; HRMS (m/z) [M þ Na]þ calcd
for C16H31NO4, 324.2151; found, 324.2161.
Preparation of 4. To a stirred solution of 9 (1.16 g, 4.02 mmol) in
dry DMF (4 mL) were added imidazole (0.82 g, 12 mmol) and DMAP
(99 mg, 0.8 mmol). After 15 min of stirring at room temperature,
TBDMSCl (1.2 g, 8.04 mmol) was introduced into the reaction mixture,
which was stirred at room temperature for 6 h. The reaction mixture was
then poured into water (10 mL) and extracted with diethyl ether (3 ꢀ
10 mL). The combined organic layers were washed with brine (10 mL)
and dried (Na2SO4). Evaporation of solvent followed by silica gel
column chromatography of the crude residue with petroleum ether/
EtOAc (95:5) as eluent afforded the silyl ether as a colorless oil in 93%
Preparation of 8. To a solution of 5 (1.09 g, 3.65 mmol) in
benzene (15 mL) was added p-toluenesulphonic acid (0.69 g, 3.65
mmol), and the mixture was heated to 50 °C. After 0.5 h the reaction
mixture was cooled to room temperature, and K2CO3 (0.5 g) was added.
After stirring for 15 min, the mixture was filtered through a short pad of
Celite, and the Celite pad was washed with diethyl ether (20 mL).
Evaporation of solvent followed by silica gel column chromatography of
the resultant residue with EtOAc/petroleum ether (2:3) gave 8 (0.63 g,
80%) as a white solid. Rf 0.4 (3:2 EtOAc/petroleum ether); mp 75-
(1.51 g) yield. Rf 0.6 (1:9 EtOAc/petroleum ether); [R]26 þ38.1 (c
D
1.10, CHCl3); IR (neat) 2953, 2930, 2858, 1761, 1749, 1253 cm-1; 1H
NMR (400 MHz, CDCl3) δ 4.25 (d, 1H, J = 3.2 Hz), 4.07 (dt, 1H, J =
7.7, 5.8 Hz), 3.89 (dd, 1H, J = 7.9, 3.3 Hz), 3.75 (s, 3H), 1.67-1.22 (m,
18H), 0.92 (s, 9H), 0.88 (t, 3H, J = 7.0 Hz), 0.10 (s, 3H), 0.05 (s, 3H);
13C NMR (100 MHz, CDCl3) δ 171.9, 109.0, 82.0, 76.5, 71.7, 52.0, 33.2,
31.8, 29.7, 29.2, 27.5, 26.6, 26.1, 25.7, 22.7, 18.4, 14.1, -4.9, -5.3;
HRMS (m/z) [M þ Na]þ calcd for C21H42O5Si, 425.2699; found,
425.2696.
77 °C; [R]26 þ128.9 (c 1.20, CHCl3); IR (KBr) 3403, 2955, 2925,
In an oven-dried 100 mL two-neck round-bottom flask equipped with
magnetic stir bar, rubber septum, and argon balloon was placed the
silyloxyester (0.95 g, 2.36 mmol) (prepared above) dissolved in 10 mL of
THF and cooled to 0 °C. LiBH4 (1.1 mL, 2 M solution in THF, 2.36
mmol) was added slowly to the reaction mixture. After the addition was
over the mixture was warmed to room temperature and stirred for
D
2856, 1773, 1465 cm-1. 1H NMR (300 MHz, CDCl3) δ 4.96 (brs, 1H),
4.51-3.97 (m, 2H), 2.68 (brs, 1H), 1.77-1.74 (m, 1H), 1.49-1.20 (m,
12H), 0.81 (t, 3H, J = 7.2 Hz); 13C NMR (100 MHz, CDCl3) δ 175.9,
81.4, 73.6, 72.9, 31.7, 29.3, 29.1, 28.9, 25.6, 22.6, 14.0; HRMS (m/z) [M
þ Na]þ calcd for C11H20O4, 239.1259; found, 239.1257.
2034
dx.doi.org/10.1021/jo102201k |J. Org. Chem. 2011, 76, 2029–2039

The Journal of Organic Chemistry
ARTICLE
another 6 h at room temperature. It was then cautiously quenched by
addition of saturated NH4Cl solution (5 mL) and extracted with diethyl
ether (3 ꢀ 10 mL). The combined organic layers were washed with brine
and dried over Na2SO4. Evaporation of the solvent and silica gel column
chromatography of the resultant residue with petroleum ether/EtOAc
(17:3) yielded 4 (0.77 g, 88%) as a colorless oil. Rf 0.5 (3:7 EtOAc/
petroleum ether); [R]26D þ15.4 (c 3.80, CHCl3); IR (neat) 3490, 2953,
(400 MHz, CDCl3) δ 4.32 (m, 1H), 3.96 (dt, 1H, J = 7.9, 3.9 Hz), 3.75
(dd, 1H, J = 7.5, 4.9 Hz), 2.61-2.45 (m, 2H), 1.77-1.27 (m, 18H), 0.87
(t, 3H, J = 6.9 Hz); 13C NMR (100 MHz, CDCl3) δ 109.1, 82.7, 81.2,
77.2, 74.0, 62.3, 33.3, 31.4, 29.2, 28.8, 27.2, 26.7, 25.6, 22.3, 13.7; HRMS
(m/z) [M þ Na]þ calcd for C15H26O3, 277.1780; found, 277.1775.
Preparation of 3. To a stirred solution of the alkyne 11 (0.2 g, 0.8
mmol) in dry acetone (5 mL) were added NBS (0.28 g, 1.6 mmol) and
AgNO3 (14 mg, 0.08 mmol), and the mixture was stirred for 1 h at room
temperature. The reaction mixture was diluted with hexane and filtered
through a pad of Celite, and the Celite pad was washed with diethyl ether
(10 mL). Residue obtained after evaporation of the solvent was purified
by silica gel column chromatography with petroleum ether/ethyl acetate
(9:1) as eluent to afford 3 as a colorless oil (0.24 g) in 90% yield. Rf 0.6
1
2930, 2858, 1376, 1253, 1062 cm-1; H NMR (400 MHz, CDCl3) δ
3.98 (dt, 1H, J = 11.3, 3.1 Hz), 3.82-3.79 (m, 1H), 3.73-3.59 (m, 3H),
2.25 (t, 1H, J = 6 Hz), 1.64-1.47 (m, 4H), 1.37 (m, 6H), 1.27-1.25 (m,
8H), 0.89 (s, 9H), 0.86 (t, 3H, J = 7.0 Hz), 0.11-0.08 (m, 6H); 13C
NMR (100 MHz, CDCl3) δ 108.3, 82.2, 76.9, 71.5, 64.1, 33.4, 31.8, 29.6,
29.1, 27.3, 26.8, 26.2, 25.8, 22.6, 18.1, 14.0, -4.60, -4.67; HRMS (m/z)
[M þ Na]þ calcd for C20H42O4Si, 397.2750; found, 397.2746.
(1:4 EtOAc/petroleum ether); [R]26 þ10.1 (c 1.00, CHCl3); IR
D
(neat) 3423, 2986, 2929, 2858, 1375, 1249 cm-1
;
1H NMR (400
Preparation of 10. A solution of the alcohol 4 (0.82 g, 2.21 mmol)
dissolved in DMSO (10 mL) was cooled to 0 °C, and IBX (0.92 g, 3.3
mmol) was introduced. The reaction mixture was then warmed to room
temperature and stirred for 3 h. After the reaction was over (indicated by
TLC), it was cooled to 0 °C and quenched with the addition of ice-cold
water (5 mL). The solids were removed by filtering through a short pad
of Celite, and the Celite pad was washed with diethyl ether (20 mL). The
filtrate was extracted with ether (20 mL). Combined ethereal extracts
were washed with brine (30 mL) and dried (Na2SO4). Evaporation of
the solvent gave the crude aldehyde which was subjected to the next
reaction without further purification.
MHz, CDCl3) δ 4.36 (t, 1H, J = 5.8 Hz), 3.94 (dt, 1H, J = 7.7, 4.0
Hz), 3.74 (dd, 1H, J = 7.4, 5.2 Hz), 2.53 (d, 1H, J = 6.5 Hz), 1.71-1.54
(m, 3H), 1.43 (s, 3H), 1.40 (s, 3H), 1.36-1.24 (m, 9H), 0.88 (t, 3H, J =
6.8 Hz); 13C NMR (100 MHz, CDCl3) δ 109.5, 83.2, 77.7, 76.6, 63.8,
46.9, 33.6, 31.7, 29.5, 29.1, 27.5, 27.0, 25.9, 22.6, 14.0; HRMS (m/z) [M
þ Na]þ calcd for C15H25BrO3, 355.0885; found, 355.0876.
Preparation of 13. To a solution of the monobenzyl alcohol 12
(1.11 g, 4.4 mmol) in THF (12 mL) were added imidazole (0.46 g, 6.6
mmol), triphenylphosphine (1.8 g, 6.6 mmol), and iodine (1.73 g, 6.6
mmol) sequentially, and the mixture was stirred at room temperature for
1 h. After the reaction was complete (indicated by TLC), it was
quenched with water (5 mL) and extracted with diethyl ether (20 ꢀ
3 mL). The combined organic layers were washed with brine (20 mL)
and dried (Na2SO4). The residue thus obtained after removal of the
solvent was purified by silica gel column chromatography to yield the
iodide (1.46 g, 92%) as a colorless oil. Rf 0.5 (5:95 EtOAc/petroleum
ether); [R]26D -8.6 (c 2.50, CHCl3); IR (neat) 3087, 3063, 3029, 2986,
To a solution of the crude aldehyde obtained above in dry CH2Cl2
(10 mL) were added CBr4 (2.93 g, 2.6 mmol) and zinc dust (0.57 g, 2.6
mmol). The reaction mixture was cooled to 0 °C, and PPh3 (2.32 g, 2.6
mmol) was added portionwise. After the addition was over, the mixture
was was stirred at 0 °C for 1 h. After completion of the reaction (TLC)
the mixture was diluted with hexane and filtered through a short pad of
Celite, and the Celite pad was washed with diethyl ether (20 mL).
Evaporation of the solvent and purification of the crude residue thus
obtained by silica gel column chromatography with petroleum ether/
EtOAc (98:2) gave 10 (0.91 g, 78% yield over two steps) as an oil. Rf 0.6
1
2865, 1453, 1371, 1215, 1094 cm-1; H NMR (300 MHz, CDCl3) δ
7.38-7.25 (m, 5H), 4.58 (s, 2H), 3.96 (dt, 1H, J = 7.2, 4.8 Hz), 3.86 (dt,
1H, J = 7.5, 5.4 Hz), 3.65 (d, 1H, J = 1.8 Hz), 3.64 (d, 1H, J = 1.5 Hz),
3.34 (dd, 1H, J = 10.8, 5.1 Hz), 3.24 (dd, 1H, J = 10.8, 5.1 Hz), 1.46 (s,
3H), 1.41 (s, 3H); 13C NMR (100 MHz, CDCl3) 137.7, 128.3, 127.7,
127.6, 109.7, 80.0, 76.5, 73.5, 70.4, 27.3, 27.2, 6.3; HRMS (m/z) [M þ
Na]þ calcd for C14H19IO3, 385.0277; found, 385.0261.
(5:95 EtOAc/petroleum ether); [R]26 þ24.4 (c 1.90, CHCl3); IR
D
(neat) 2986, 2955, 2930, 2858, 1253 cm-1
;
1H NMR (400 MHz,
CDCl3) δ 6.58 (d, 1H, J = 10.5 Hz), 4.45 (dd, 1H, J = 8.5, 3.7 Hz), 4.04
(dt, 1H, J = 13.5, 6.1 Hz), 3.70 (dd, 1H, J = 7.8, 3.7 Hz), 1.66-1.38 (m,
18H), 0.98-0.92 (m, 12H), 0.16-0.15 (m, 6H); 13C NMR (100 MHz,
CDCl3) δ 138.5, 109.0, 90.3, 83.3, 76.5, 73.2, 33.6, 31.8, 29.6, 29.2, 27.6,
26.7, 26.0, 25.8, 22.7, 18.1, 14.1, -4.4, -4.9; HRMS (m/z) [M þ Na]þ
calcd for C21H40Br2O3Si, 551.1098; found, 551.1058.
To a solution of the iodide (prepared above) in THF were added zinc
dust(2.16 g, 33.2 mmol) and AcOH (2.4 mL, 41.5 mmol), andthemixture
was stirred at room temperature. Progress of the reaction was monitored
by TLC, and after reaction was complete (∼6 h) the solid residue was
filtered through a Celite pad, and the Celite pad was washed with ether
(40 mL). The filtrate was diluted with water (10 mL) and then extracted
with ether (3 ꢀ 10 mL). The combined organic layers were washed with
brine (20 mL) and dried (Na2SO4). The residue obtained after removal of
the solvent was purified by silica gel column chromatography to yield 13
(64 g, 87%) as a colorless oil. Rf 0.5 (3:17 EtOAc/petroleum ether);
[R]26D þ5.0 (c 1.80, CHCl3); IR (neat) 3432, 2860, 1453, 1105 cm-1; 1H
NMR (300 MHz, CDCl3) δ 7.35-7.29 (m, 5H), 5.80 (ddd, 1H, J = 17.4,
10.8, 5.7 Hz), 5.35 (dt, 1H, J = 17.4, 1.5 Hz), 5.19 (dt, 1H, J= 10.8, 1.5 Hz),
4.56 (s, 2H), 4.37-4.31 (m, 1H), 3.53 (dd, 1H, J = 9.6, 3.3 Hz), 3.37 (dd,
1H, J = 9.6, 7.8 Hz), 2.62 (brs, 1H); 13C NMR (75 MHz, CDCl3) δ 137.8,
136.5, 128.4, 127.8, 127.7, 116.4, 74.0, 73.3, 71.4; HRMS (m/z) [M þ
Na]þ calcd for C11H14O2, 201.0891; found, 201.0899.
Preparation of 14. To a stirred solution of 13 (0.62 g, 2.5 mmol) in
dry DMF (2 mL) were added imidazole (0.72 g, 10.5 mmol) and DMAP
(86 mg, 0.7 mmol). After 15 min of stirring at room temperature,
TBDPSCl (1.8 mL, 7 mmol) was introduced into the reaction mixture,
and the mixture was stirred at room temperature for 6 h. The reaction
mixture was then poured into water (10 mL) and extracted with diethyl
ether (3 ꢀ 10 mL). The combined organic layers were washed with brine
(10 mL) and dried (Na2SO4). Evaporation of solvent followed by silica
Preparation of 11. To a solution of 10 (0.68 g, 1.3 mmol) in dry
THF (10 mL) was slowly added n-BuLi (1.5 mL, 3.3 mmol, 2.6 M in
THF) at -78 °C, and the mixture was stirred at the same temperature
for 30 min. The reaction mixture was warmed to 0 °C and stirred for
another 0.5 h. It was allowed to come to room temperature and stirred
for 0.5 h. Then it was cooled to 0 °C and cautiously quenched with water
(5 mL). The reaction mixture was extracted with diethyl ether (3 ꢀ
10 mL) and the ethereal extracts were washed with brine (10 mL) and
dried over Na2SO4. Evaporation of the solvent followed purification of
the resulting residue by silica gel column chromatography with petro-
leum ether/EtOAc (98:2) as eluent afforded the alkyne as a colorless oil.
To a precooled (0 °C) solution of the silyloxy alkyne (obtained
above) in THF (5 mL) was added TBAF (2.6 mL of 1 M in THF, 2.6
mmol), and the mixture was stirred at room temperature. After 1 h the
reaction mixture was quenched with water (5 mL) and extracted with
diethyl ether (3 ꢀ 10 mL). Combined organic layers were washed with
brine and then dried over Na2SO4. Evaporation of the solvent and
purification with silica gel column chromatography with petroleum
ether/EtOAc (9:1) gave the alcohol 11 (0.21 g, 65% yield) as a colorless
oil. Rf 0.6 (1:4 EtOAc/petroleum ether); [R]26D -25.3 (c 1.10, CHCl3);
1
IR (neat) 3436, 3311, 2985, 2929, 2859, 1599, 1380 cm-1; H NMR
2035
dx.doi.org/10.1021/jo102201k |J. Org. Chem. 2011, 76, 2029–2039

The Journal of Organic Chemistry
ARTICLE
gel column chromatography of the crude residue with petroleum ether/
ethyl acetate (98:2) as eluent afforded the silyl ether 14 (1.43 g, 98%) as
a colorless oil. Rf 0.6 (5:95 EtOAc/petroleum ether); [R]26D þ27.7 (c
2.70, CHCl3); IR (neat) 3070, 2932, 2858, 1111 cm-1; 1H NMR (300
MHz, CDCl3) δ 7.74-7.67 (m, 4H), 7.44-7.21 (m, 11H), 5.93 (ddd,
1H, J = 17.4, 10.8, 6.0 Hz), 5.24 (dt, 1H, J = 17.4, 1.5 Hz), 5.12 (dt, 1H,
J = 10.2, 1.5 Hz), 4.41-4.35 (m, 3H), 3.47 (dd, 1H, J = 9.9, 5.7 Hz), 3.39
(dd, 1H, J = 9.9, 5.7 Hz), 1.11 (s, 9H); 13C NMR (75 MHz, CDCl3) δ
138.3, 136.0, 135.9, 134.2, 133.8, 129.5, 128.2, 127.5, 127.4, 115.5, 74.6,
73.3, 73.1, 27.0, 19.3; HRMS (m/z) [M þ Na]þ calcd for C27H32O2Si,
439.2069; found, 439.2060.
Preparation of 15. To a solution of 14 (1.1 g, 2.65 mmol) in a 10:1
mixture of CH2Cl2/H2O (20 mL) was added DDQ (3 g, 13.3 mmol),
and the mixture was stirred at room temperature for 24 h. After the
reaction was complete (indicated by TLC), the reaction mixture was
passed through a pad of Celite, and the Celite pad was washed with ether
(40 mL). The filtrate was washed with a saturated solution of NaHCO3
(2 ꢀ 10 mL) and brine (10 mL), dried over Na2SO4, and concentrated
in vacuo. The crude product was purified by column chromatography
using petroleum ether/ethyl acetate (4:1) as eluent to yield the alcohol
15 in 81% (0.7 g) yield as a colorless oil. Rf 0.5 (1:9 EtOAc/petroleum
ether); [R]26D -7.0 (c 3.10, CHCl3); IR (neat) 3422, 2932, 2858, 1427,
1110 cm-1; 1H NMR (400 MHz, CDCl3) δ 7.71-7.68 (m, 4H), 7.45-
7.37 (m, 6H), 5.83 (ddd, 1H, J = 16.8, 10.5, 6.2 Hz), 5.17-5.09 (m, 2H),
4.28 (m, 1H), 3.50 (t, 2H, J = 5.6 Hz), 1.82 (t, 1H, J = 6.3 Hz), 1.11 (s,
9H); 13C NMR (100 MHz, CDCl3) δ 137.3, 135.8, 135.6, 133.7, 133.3,
129.8, 129.7, 127.6, 127.5, 116.6, 75.1, 66.5, 26.9, 19.3; HRMS (m/z) -
[M þ Na]þ calcd for C20H26O2Si, 349.1600; found, 349.1615.
reaction mixture turns blue). The reaction mixture was allowed to warm
to room temperature. After stirring for 1 h, the solution was extracted
with CH2Cl2 (3 ꢀ 10 mL), dried over Na2SO4, and concentrated in
vacuo. The crude product was purified by column chromatography using
petroleum ether/EtOAc (95:5) to yield the desired product 16 (0.15 g)
in 45% yield. Rf 0.4 (1:9 EtOAc/petroleum ether); [R]26 þ131.3 (c
D
2.70, CHCl3); IR (neat) 3420, 2985, 2954, 2930, 2858, 1372, 1249,
1113, 1059 cm-1; 1H NMR (400 MHz, CDCl3) δ 7.69-7.60 (m, 4H),
7.38-7.21 (m, 6H), 5.79 (ddd, 1H, J = 16.7, 10.1, 5.4 Hz), 5.23 (d, 1H,
J = 16.9 Hz), 5.08 (d, 1H, J = 10.1 Hz), 4.79 (d, 1H, J = 5.3 Hz), 4.35 (brs,
1H), 3.90 (dt, 1H, J = 7.7, 4.1 Hz), 3.69 (dd, 1H, J = 7.5, 4.7 Hz), 2.47
(brs, 1H), 1.60-1.22 (m, 18 H), 1.04-1.02 (m, 9H), 0.82 (t, 3H, J = 6.9
Hz); 13C NMR (100 MHz, CDCl3) δ 136.2, 135.9, 135.7, 133.0, 132.8,
129.9, 127.62, 127.60, 116.1, 109.5, 83.0, 79.1, 77.6, 70.3, 69.4, 64.9, 63.2,
33.5, 31.7, 29.5, 29.1, 27.5, 27.0, 26.8, 26.5, 25.9, 22.6, 19.3, 14.0; HRMS
(m/z) [M þ Na]þ calcd for C36H48O4Si, 595.3223; found, 595.3220.
Preparation of 1c. To a solution of 16 (17 mg, 0.03 mmol) in
MeOH (1 mL) was added 4 N HCl (1 mL), and the mixture was stirred
at room temperature for 6 h. After reaction was over (indicated by TLC),
MeOH was removed under reduced pressure, water (5 mL) was added,
and the mixture was extracted with EtOAc (3 ꢀ 5 mL). The combined
organic layers were washed with brine (5 mL) and dried over Na2SO4.
Evaporation of the solvent and purification of the crude residue by silica
gel column chromatography using petroleum ether/EtOAc (7:3) gave
the diyne tetrol 1c in 69% (6 mg) yield as yellow oil. Rf 0.5 (3:2 EtOAc/
petroleum ether); [R]26D -27.6 (c 0.60, MeOH); IR (neat) 3368, 2927,
2858, 1662, 1457, 1024 cm-1; 1H NMR (400 MHz, CDCl3 þ D2O) δ
5.92 (ddd, 1H, J = 16.9, 10.1, 5.3 Hz), 5.46 (d, 1H, J = 17.0 Hz), 5.25 (d,
1H, J = 10.1 Hz), 4.93 (d, 1H, J = 5.2 Hz), 4.56 (d, 1H, J = 6.0 Hz), 3.86-
3.79 (m, 1H), 3.53 (d, 1H, J = 4.8 Hz), 1.60-1.27 (m, 16H), 0.88 (t, 3H,
J = 7.0 Hz); 13C NMR (100 MHz, CDCl3) δ 135.5, 117.2, 78.5, 78.0,
75.6, 71.2, 70.1, 69.8, 64.4, 62.9, 33.8, 31.7, 29.4, 29.1, 25.5, 22.5, 13.9;
HRMS (m/z) [M þ Na]þ calcd for C17H26O4, 317.1729; found,
317.1714.
Preparation of 2a. The alcohol 15 (1.5 g, 4.6 mmol) dissolved in
DMSO (8 mL) was cooled to 0 °C, and IBX (2.6 g, 9.2 mmol) was added
to it. The reaction mixture was warmed to room temperature and stirred
for 3 h. After the reaction was over, the mixture was cooled to 0 °C and
was quenched with ice-cold water (8 mL). The solids were filtered
through a short pad of Celite, and the Celite pad was washed with diethyl
ether (20 mL). The filtrate was extracted with ether (20 mL). Combined
ethereal extracts were washed with brine (30 mL) and dried (Na2SO4).
Evaporation of the solvent gave the crude aldehyde, which was subjected
to the next reaction without further purification.
To a solution of the aldehyde in dry methanol (10 mL) was added a
dissolved solution of Ohira-Bestmann reagent (2.02 g, 9.2 mmol) in
MeOH (5 mL), and the mixture was cooled to 0 °C. Then K2CO3 (1.27 g,
9.2 mmol) was introduced into the reaction mixture, which was stirred at
room temperature for another 3 h. After reaction was complete (TLC),
the reactionmixturewasdilutedwith hexane (5 mL) and filteredthrougha
short pad of Celite, and the Celite pad was washed with diethyl ether
(10 mL). Evaporation of the solvent and silica gel column chromatogra-
phy of the residue with petroleum ether/EtOAc (98:2) as eluent afforded
the alkyne 2a in 27% (0.39 g) yield as a colorless oil. Rf 0.7 (2:98 EtOAc/
petroleum ether); [R]26D þ46.8 (c 1.10, CHCl3) [lit. [R]D þ45.6 (c 2.9,
CHCl3)]; IR (neat) 3304, 2959, 2859, 1428, 1111, 1069 cm-1; 1H NMR
(300 MHz, CDCl3) δ 7.77-7.67 (m, 4H), 7.45-7.34 (m, 6H), 5.89
(ddd, 1H, J = 17.1, 10.2, 5.1 Hz), 5.32 (dt, 1H, J = 16.8, 1.5 Hz), 5.12 (dd,
1H, J = 10.5, 1.5 Hz), 4.82 (m, 1H), 2.44 (d, 1H, J = 2.1 Hz), 1.09 (s, 9H);
13C NMR (100 MHz, CDCl3) δ 137.1, 136.0, 135.8, 133.2, 133.1, 129.8,
127.6, 127.5, 115.6, 83.0, 73.8, 64.3, 26.8, 19.3; HRMS (m/z) [M þ Na]þ
calcd for C21H24OSi, 343.1494; found, 343.1484.
Preparation of 18. To a precooled (0 °C) solution of 9 (0.5 g, 1.74
mmol) in dry THF (20 mL) were added triphenylphosphine (1.83 g,
6.96 mmol) and p-nitro benzoic acid (1.16 g, 6.96 mmol mmol) under
argon atmosphere, and the mixture was stirred for 10 min. DIAD
(1.4 mL, 6.96 mmol) was introduced into the reaction mixture over a
period of 20 min at the same temperature. The reaction mixture was
warmed to room temperature and stirred for 5 h. After the reaction was
complete (TLC), the solvent was removed under reduced pressure, and
the crude ester thus obtained was purified by column chromatography to
yield the corresponding p-nitro benzoate ester (0.67 g, 87%) as pale
yellow oil. Rf 0.5 (1:9 EtOAc/petroleum ether); [R]26D þ23.5 (c 1.60,
CHCl3); IR (neat) 2930, 2857, 1736, 1531 cm-1; 1H NMR (400 MHz,
CDCl3) δ 8.34-8.27 (m, 4H), 5.46 (d, 1H, J = 3.0 Hz), 4.30-4.26 (m,
1H), 4.15 (dd, 1H, J = 7.8, 3.1 Hz), 3.82 (s, 3H), 1.61-1.27 (m, 18H),
0.88 (t, 3H, J = 6.9 Hz); 13C NMR (100 MHz, CDCl3) δ 167.2, 163.8,
134.2, 130.9, 123.5, 109.6, 79.6, 77.0, 72.9, 52.6, 33.2, 31.7, 29.4, 29.0,
27.4, 26.7, 25.8, 22.5, 13.9; HRMS (m/z) [M þ Na]þ calcd for
C22H31NO8, 460.1947; found, 460.1960.
To a solution of the benzoate (obtained above) (0.71 g, 1.63 mmol)
in methanol (10 mL) was added K2CO3 (0.23 g) at 0 °C, and the
mixture was stirred for 15 min. The reaction mixture was then filtered
through a short pad of Celite. The Celite pad was washed with diethyl
ether (20 mL). The crude residue obtained by evaporation of solvent
was purified by silica gel column chromatography with petroleum ether/
ethyl acetate (1:9) as eluent to afford the hydroxy ester 18 (0.44 g, 94%
Preparation of 16. To a stirred solution of n-BuNH2 (4.7 mL, 48
mmol) and distilled water (11 mL) was added Cu(I) chloride (10 mg,
0.1 mmol) under a flow of N2 at 0 °C, which resulted in a deep blue
solution. A few crystals of NH2OH HCl were added to get a colorless
yield) as a colorless oil. Rf 0.6 (1:4 EtOAc/petroleum ether); [R]26
D
3
solution, which is indicative of the presence of the required Cu(I) salt. A
solution of 2a (0.17 g, 0.53 mmol) in CH2Cl2 (5 mL) was added at 0 °C
resulting in a yellow suspension. Then 3 (0.19 g, 0.58 mmol) in CH2Cl2
þ41.7 (c 2.60, CHCl3); IR (neat) 3461, 2930, 2858, 1744, 1371, 1217,
1071 cm-1; 1H NMR (400 MHz, CDCl3) δ 4.35 (dd, 1H, J = 5.8, 3.5
Hz), 4.1 (m, 1H), 3.88 (dd, 1H, J = 8.0, 3.5 Hz), 3.82 (s, 3H),
2.98 (d, 1H, J = 5.9 Hz), 1.55-1.42 (m, 3H), 1.40 (s, 3H), 1.37
was slowly added (NH2OH HCl should be added whenever color of the
3
2036
dx.doi.org/10.1021/jo102201k |J. Org. Chem. 2011, 76, 2029–2039

The Journal of Organic Chemistry
ARTICLE
(s, 3H), 1.31-1.23 (brm, 9H), 0.88 (t, 3H, J = 7.0 Hz); 13C NMR (100
MHz, CDCl3) δ 172.3, 109.0, 82.0, 76.6, 70.7, 52.6, 33.3, 31.7, 29.5, 29.1,
27.4, 26.7, 25.8, 22.6, 14.0; HRMS (m/z) [M þ Na]þ calcd for
C15H28O5, 311.1834; found, 311.1832.
124.9, 108.6, 78.4, 78.1, 69.9, 64.2, 32.2, 31.3, 28.6, 27.7, 25.2, 22.4, 14.0;
HRMS (m/z) [M þ Na]þ calcd for C14H26O4, 281.1729; found,
281.1722.
Preparation of 22. To a solution of 24 (0.33 g, 1.28 mmol) in 2 mL
of MeOH at room temperature was added activated 10% Pd/C (60 mg).
The reaction mixture was stirred for 3 h under hydrogen atmosphere
(balloon) at the same temperature. After the reaction was complete
(TLC), the mixture was filtered through a short pad of Celite, and the
Celite pad was washed with ether (15 mL). Evaporation of solvent
followed by column chromatography of the resulting residue using
petroleum ether/EtOAc (7:3) yielded 22 (0.3 g, 96%) as a colorless oil.
Rf 0.4 (3:2 EtOAc/petroleum ether); [R]26D -8.3 (c 3.20, CHCl3); IR
(neat) 3401, 2927, 2858, 1379 cm-1; 1H NMR (300 MHz, CDCl3) δ
4.23-4.16 (m, 1H), 3.98-3.92 (m, 1H), 3.84-3.72 (m, 3H), 2.60-
2.30 (brm, 2H), 1.82-1.62 (m, 3H), 1.58-1.49 (m, 15H), 0.88 (t, 3H,
J = 7.0 Hz); 13C NMR (100 MHz, CDCl3) δ 108.0, 77.9, 77.7, 69.6, 64.7,
31.8, 29.6, 29.3, 29.2, 28.0, 26.6, 25.6, 22.6, 14.0; HRMS (m/z) [M þ
Na]þ calcd for C14H28O4, 283.1885; found, 283.1873.
Preparation of 19. To a stirred solution of 18 (0.42 g, 1.46 mmol)
in dry DMF (4 mL) were added imidazole (0.3 g, 4.38 mmol) and
DMAP (35 mg, 0.29 mmol). After 15 min of stirring at room
temperature, TBDMSCl (0.44 g, 2.92 mmol) was introduced into the
reaction mixture, which was stirred at room temperature for 6 h. The
reaction mixture was then poured into water (10 mL) and extracted with
diethyl ether (3 ꢀ 10 mL). The combined organic layers were washed
with brine (10 mL) and dried (Na2SO4). Evaporation of solvent
followed by silica gel column chromatography of the crude residue with
petroleum ether/ethyl acetate (95:5) as eluent afforded the silyl ether 19
as a colorless oil in 94% (0.32 g) yield. Rf 0.6 (1:9 EtOAc/petroleum
ether); [R]26 -0.39 (c 3.90, CHCl3); IR (neat) 2931, 2859, 1760,
D
1464, 1255 cm-1; 1H NMR (400 MHz, CDCl3) δ 4.29 (d, 1H, J = 4.7
Hz), 4.10-4.00 (m, 1H), 3.88-3.85 (m, 1H), 3.73 (s, 3H), 1.53-1.22
(m, 18H), 0.95-0.82 (m, 12H), 0.06 (s, 6H); 13C NMR (100 MHz,
CDCl3) δ 171.8, 108.9, 81.6, 77.7, 73.0, 51.8, 33.8, 31.7, 29.6, 29.1, 27.5,
27.0, 26.0, 25.6, 22.6, 18.2, 14.0, -5.1, -5.2; HRMS (m/z) [M þ Na]þ
calcd for C21H42O5Si, 425.2699; found, 425.2696.
Preparation of 25. To a stirred solution of the diol 22 (0.7 g, 2.7
mmol) in toluene (15 mL) was added (n-Bu)2SnO (0.67 g, 2.7 mmol),
and the mixture was was refluxed using a Dean-Stark water trap for
18 h. BnBr (0.3 mL, 2.7 mmol) and TBAI (tetra n-butyl ammonium
iodide) (2 g) were added to the reaction mixture and refluxed for further
2 h. The mixture was was cooled to room temperature, and most of the
toluene was removed under reduced pressure. The residue thus obtained
was diluted with CH2Cl2 (10 mL), water (5 mL) was added to it, and the
mixture was extracted with CH2Cl2 (3 ꢀ 10 mL). The combined organic
layers were washed with brine and dried over Na2SO4. Evaporation of
the solvent followed by column chromatography of the resulting residue
using petroleum ether/EtOAc (9:1) yielded the benzyloxy ether (0.75 g,
Preparation of 20. Diyne was synthesized following the same
procedure described for the synthesis of 16. Yield 58% (colorless oil). Rf
0.4 (1:9 EtOAc/petroleum ether); [R]26D þ130.5 (c 2.30, CHCl3); IR
(neat) 3418, 2955, 2858, 1111, 1060 cm-1 1H NMR (400 MHz,
;
CDCl3) δ 7.73-7.64 (m, 4H), 7.42-7.36 (m, 6H), 5.84 (ddd, 1H, J =
16.6, 10.5, 5.3 Hz), 5.38 (d, 1H, J = 16.9 Hz), 5.13 (d, 1H, J = 10.2 Hz),
4.83 (d, 1H, J = 5.2 Hz), 4.52-4.51 (m, 1H), 4.02 (dt, 1H, J = 8.0, 4.0
Hz), 3.78 (dd, 1H, J = 3.5, 7.9 Hz), 2.57 (brs, 1H), 1.42-1.26 (m, 18 H),
1.08-1.06 (s, 9H), 0.87 (t, 3H, J = 7 Hz); 13C NMR (100 MHz, CDCl3)
δ 136.2, 135.9, 135.7, 134.8, 133.0, 132.7, 129.9, 129.6, 127.62, 127.60,
127.5, 116.1, 109.3, 82.5, 78.9, 77.3, 76.4, 71.1, 69.3, 64.9, 63.0, 33.7,
31.7, 29.5, 29.1, 27.5, 26.86, 26.80, 26.5, 25.9, 22.6, 19.3, 14.1; HRMS
(m/z) [M þ Na]þ calcd for C36H48O4Si, 595.3220; found, 595.3220.
Preparation of 1e. Compound 1e was synthesized following a
similar procedure described for the synthesis of 1c. Yield 69% (white
80%) as a colorless oil. Rf 0.6 (1:4 EtOAc/petroleum ether); [R]26
D
þ0.2 (c 2.60, CHCl3); IR (neat) 3582, 3467, 2984, 2927, 2858, 1454,
1378 cm-1; 1H NMR (400 MHz, CDCl3) δ 7.37-7.26 (m, 5H), 4.58 (s,
2H), 4.17 (ddd, 1H, J = 9.4, 5.4, 3.6 Hz), 3.95 (dd, 1H, J = 9.1, 5.1 Hz),
3.87-3.83 (m, 1H), 3.74 (dd, 1H, J = 9.5, 2.7 Hz), 3.57 (dd, 1H, J = 9.4,
6.7 Hz), 2.48 (d, 1H, J = 4.8 Hz), 1.72-1.27 (m, 18H), 0.87 (t, 3H, J =
6.9 Hz); 13C NMR (100 MHz, CDCl3) δ 137.9, 128.4, 127.7, 107.8,
78.1, 77.4, 73.4, 72.1, 68.4, 31.8, 29.6, 29.3, 29.2, 28.2, 26.5, 25.7, 22.6,
14.0; HRMS (m/z) [M þ Na]þ calcd for C22H34O4, 373.2355; found,
373.2355.
solid). Rf 0.5 (3:2 EtOAc/petroleum ether); mp 77-78 °C; [R]26
-
D
31.7 (c 1.50, MeOH); IR (KBr) 3352, 2954, 2925, 2856, 1611, 1415,
1022 cm-1; 1H NMR (400 MHz, CD3OD) δ 5.92 (ddd, 1H, J = 16.7,
10.1, 5.5 Hz), 5.40 (d, 1H, J = 17.0 Hz), 5.19 (d, 1H, J = 10 Hz), 4.43 (d,
1H, J = 7.3 Hz), 3.82-3.70 (m, 1H), 3.38 (brd, 1H, J = 7.5 Hz), 3.32
(brs, 1H), 1.53-1.32 (m, 12H), 0.90 (t, 3H, J = 7 Hz); 13C NMR (100
MHz, CD3OD) δ 138.1, 116.6, 81.4, 78.9, 76.6, 71.0, 70.4, 69.5, 64.6,
63.8, 34.5, 33.0, 30.7, 30.4, 26.9, 23.7, 14.4; HRMS (m/z) [M þ Na]þ
calcd for C17H26O4, 317.1729; found, 317.1734.
To a stirred solution of the benzyloxy ether (0.42 g, 1.22 mmol) in dry
DMF (5 mL) were added imidazole (0.25 g, 3.7 mmol) and DMAP (30
mg, 0.24 mmol). After 15 min of stirring at room temperature,
TBDMSCl (0.37 g, 2.44 mmol) was introduced into the reaction
mixture, which was stirred at room temperature for 6 h. The reaction
mixture was then poured into water (10 mL) and extracted with diethyl
ether (3 ꢀ 10 mL). The combined organic layers were washed with brine
(10 mL) and dried (Na2SO4). Evaporation of solvent followed by silica
gel column chromatography of the crude residue with petroleum ether/
ethyl acetate (95:5) as eluent afforded the silyl ether 25 (0.51 g, 90%) as
Preparation of 24. In an oven-dried two-neck 100 mL round-
bottom flask equipped with a magnetic stir bar, rubber septum, and
argon inlet was placed hexyltriphenylphosphonium bromide (3.37 g, 7.9
mmol). THF (10 mL) was added, and the mixture was was cooled to
0 °C. n-BuLi (3 mL, 2.6 M in hexane, 7.9 mmol) was added slowly
dropwise to the reaction mixture, which was stirred for 15 min. A
solution of the lactol 23 (0.5 g, 2.63 mmol) dissolved in THF (8 mL) was
introduced into the reaction mixture. The resulting solution was stirred
at room temperature for 16 h, cooled to 0 °C, slowly quenched with a
saturated solution of citric acid (10 mL), and extracted with diethyl ether
(3 ꢀ 10 mL) . The combined ethereal layers were washed with brine
(10 mL) and dried over Na2SO4. Evaporation of the solvent followed by
column chromatography of the resulting residue using petroleum ether/
EtOAc (7:3) yielded 24 (0.4 g, 60%) as a colorless oil (E/Z = 3:7). Rf 0.4
a colorless oil. Rf 0.7 (1:9 EtOAc/petroleum ether); [R]26 -25.1 (c
D
3.00, CHCl3); IR (neat) 2954, 2929, 2857, 1461, 1249, 1101 cm-1; 1H
NMR (400 MHz, CDCl3) δ 7.34-7.26 (m, 5H), 4.53 (s, 2H), 4.15-
4.01 (m, 2H), 3.97 (dt, 1H, J = 7.6, 1.8 Hz), 3.70 (dd, 1H, J = 10.0, 1.8
Hz), 3.52 (dd, 1H, J = 10.0, 5.9 Hz), 1.60-1.28 (m, 18 H), 0.89-0.86
(m, 12H), 0. 09 (s, 3H), 0.08 (s, 3H); 13C NMR (100 MHz, CDCl3) δ
138.3, 128.2, 127.5, 127.4, 107.8, 78.0, 77.8, 73.3, 73.2, 71.0, 31.8, 29.8,
29.6, 29.2, 28.3, 26.1, 26.0, 25.9, 22.6, 18.2, 14.1, -3.6, -5.0; HRMS
(m/z) [M þ Na]þ calcd for C27H48O4Si, 487.3220; found, 487. 3223.
Preparation of 26. To a solution of 25 (0.5 g, 1.1 mmol) in EtOAc
(6 mL) at room temperature was added activated 10% Pd/C (90 mg).
The reaction mixture was stirred for 8 h under hydrogen atmosphere
(balloon). After the reaction was complete (TLC), it was filtered
through a short pad of Celite, and the Celite pad was washed with ether
(3:2 EtOAc/petroleum ether); IR (neat) 3413, 2956, 2928, 1379 cm-1
;
1H NMR (400 MHz, CDCl3) δ 5.89-5.51 (m, 2H), 4.63 (m, 1H),
4.17-3.61 (m, 2H), 2.58 (brs, 2H), 2.20-2.01 (m, 3H), 1.43-1.01 (m,
14H), 0.71 (t, 3H, J = 7.0 Hz); 13C NMR (100 MHz, CDCl3) δ 136.6,
2037
dx.doi.org/10.1021/jo102201k |J. Org. Chem. 2011, 76, 2029–2039

The Journal of Organic Chemistry
ARTICLE
(15 mL). Evaporation of the solvent followed by column chromatogra-
phy of the resulting residue using petroleum ether/EtOAc (9:1) yielded
26 (0.38 g, 94%) as a colorless oil. Rf 0.5 (3:7 EtOAc/petroleum ether);
(3 ꢀ 10 mL). Combined organic layers were washed with brine and
dried over Na2SO4. Evaporation of the solvent and purification of the
resultant residue by silica gel column chromatography with petroleum
ether/ethyl acetate (9:1) gave the alcohol 29 (0.1 g, 93% yield) as a
colorless oil. Rf 0.6 (1:4 EtOAc/petroleum ether); [R]26D þ37.4 (c 2.20,
1H NMR (400 MHz, CDCl3) δ 4.36 (dt, 1H, J = 7.1, 1.9 Hz), 4.20 (dt,
1H, J = 8.8, 5.8 Hz), 4.09 (t, 1H, J = 5.8 Hz), 2.54 (d, 1H, J = 2.0 Hz), 2.31
(brd, 1H, J = 6.7 Hz), 1.76-1.66 (m, 3H), 1.57-1.51 (m, 3H), 1.37 (s,
3H), 1.35-1.20 (m, 9H), 0.87 (t, 3H, J = 7.0 Hz); 13C NMR (100 MHz,
CDCl3) δ 108.4, 89.7, 79.5, 77.3, 74.8, 62.2, 31.7, 29.5, 29.1, 28.8, 27.4,
26.9, 25.3, 22.6, 14.0; HRMS (m/z) [M þ Na]þ calcd for C15H26O3,
277.1780; found, 277.1770.
Preparation of 21. To a solution of the alkyne 29 (98 mg, 0.39
mmol) in dry acetone (6 mL) were added NBS (139 mg, 0.78 mmol)
and AgNO3 (7 mg, 0.039 mmol), and the mixture was stirred for 1 h at
room temperature and diluted with hexane. The mixture was filtered
through a pad of Celite, and the Celite pad was washed with diethyl ether
(10 mL). Residue obtained after evaporation of the solvent was further
purified by silica gel column chromatography with petroleum ether/
ethyl acetate (9:1) as eluent to afford 21 as a colorless oil in (125 mg)
98% yield. Rf 0.6 (1:4 EtOAc/petroleum ether); [R]26D þ46.5 (c 1.80,
CHCl3); IR (neat) 3417, 2952, 2926, 2857, 1379, 1219, 1058 cm-1; 1H
NMR (400 MHz, CDCl3) δ 4.40-4.30 (brm, 1H), 4.19 (dt, 1H, J = 9.1,
6.0 Hz), 4.08 (t, 1H, J = 5.8 Hz), 2.56-2.50 (brs, 1H), 1.76-1.63 (m,
2H), 1.56-1.50 (m, 4H), 1.36-1.26 (m, 12H), 0.87 (t, 3H, J = 6.9 Hz);
13C NMR (100 MHz, CDCl3) δ 108.5, 79.7, 78.9, 77.2, 63.3, 46.9, 31.7,
29.5, 29.1, 28.7, 27.3, 26.9, 25.3, 22.6, 14.0; HRMS (m/z) [M þ Na]þ
calcd for C15H25BrO3, 355.0885; found, 355. 0872.
[R]26 -37.3 (c 3.80, CHCl3); IR (neat) 3488, 2954, 2930, 2858,
D
1464 cm-1; 1H NMR (400 MHz, CDCl3) δ 4.15-4.05 (m, 2H), 3.81
(dt, 1H, J = 8.1, 3.8 Hz), 3.77-3.62 (m, 2H), 2.28 (dd, 1H, J = 8.7, 4.0
Hz), 1.72-1.43 (m, 3H), 1.42 (s, 3H), 1.34 (s, 3H), 1.32-1.25 (m, 9H),
0.87-0.85 (m, 12H), 0.12 (s, 3H), 0.10 (s, 3H); 13C NMR (100 MHz,
CDCl3) δ 108.1, 79.3, 77.9, 70.5, 65.4, 31.8, 29.8, 29.6, 29.2, 28.1, 26.2,
25.8, 22.6, 18.0, 14.0, -3.5, -4.7; HRMS (m/z) [M þ Na]þ calcd for
C20H42O4Si, 397.2750; found, 397.2756.
CHCl3); IR(neat) 3431, 3311, 2953, 2927, 2858, 1376, 1220, 1052cm-1
;
Preparation of 27. To a solution of the alcohol 26 (0.5 g, 1.35
mmol) was dissolved in DMSO (6 mL) and was cooled to 0 °C and IBX
(0.76 g, 2.7 mmol) was added. The reaction mixture was then warmed to
room temperature and stirred for 3 h at room temperature. After the
reaction was over, the reaction mixture was cooled to 0 °C and quenched
with ice-cold water (5 mL). The solids were filtered through a short pad
of Celite, and the Celite pad was washed with diethyl ether (20 mL). The
filtrate was extracted with diethyl ether (3 ꢀ 10 mL). Combined ethereal
extracts were washed with brine (30 mL) and dried (Na2SO4).
Evaporation of the solvent gave the crude aldehyde, which was subjected
to the next reaction without further purification.
To a solution of the crude aldehyde obtained above in dry CH2Cl2
(5 mL) were added CBr4 (1.79 g, 5.4 mmol) and zinc dust (0.35 g, 5.4
mmol). The reaction mixture was cooled to 0 °C, PPh3 (1.4 g, 5.4 mmol)
was added portionwise to it, and the mixture was stirred at 0 °C for
further 1 h. Then it was diluted with hexane and filtered through a short
pad of Celite, and the Celite pad was washed with diethyl ether (20 mL).
Evaporation of the solvent and purification of the crude residue thus
obtained by silica gel column chromatography using petroleum ether/
EtOAc (98:2) gave 27 in 62% (0.44 g) yield. Rf 0.6 (5:95 EtOAc/
petroleum ether); [R]26D þ3.4 (c 2.80, CHCl3); IR (neat) 2955, 2929,
2858, 1253, 1070 cm-1; 1H NMR (400 MHz, CDCl3) δ 6.37 (d, 1H, J =
8.8 Hz), 4.35 (t, 1H, J = 8.6 Hz) 4.16-4.11 (m, 1H), 3.88 (dd, 1H, J =
8.0, 5.7 Hz), 1.66-1.27 (m, 18 H), 0.89-0.86 (m, 12H), 0.12 (s, 3H),
0.09 (s, 3H); 13C NMR (100 MHz, CDCl3) δ 139.2, 107.9, 91.6, 79.4,
77.8, 71.9, 31.8, 29.6, 29.5, 29.2, 28.0, 26.9, 25.8, 25.4, 22.6, 17.9, 14.1,
-3.8, -4.8; HRMS (m/z) [M þ Na]þ calcd for C21H40Br2O3Si,
551.1098; found, 551.1124.
’ ASSOCIATED CONTENT
S
Supporting Information. General experimental proce-
b
dures and spectroscopic data for the compounds and copies of
1H NMR and 13C NMR spectra. This material is available free of
charge via the Internet at http://pubs.acs.org.
’ AUTHOR INFORMATION
Corresponding Author
*Fax: þ918023600529. E-mail: prasad@orgchem.iisc.ernet.in.
Preparation of 28. To a dissolved solution of 27 (0.32 g, 0.6
mmol) in dry THF (8 mL) was added n-BuLi (0.7 mL, 1.8 mmol, 2.6 M
in hexane) slowly at -78 °C and stirred at the same temperature for 30
min. The reaction mixture was then warmed to 0 °C, stirred 0.5 h at 0 °C
and was allowed to come to room temperature. Progress of the reaction
was monitored by TLC and after 0.5 h at room temperature it was cooled
to 0 °C and was cautiously quenched with water (5 mL). It was extracted
with diethyl ether (3 ꢀ 10 mL) and ethereal extracts were washed with
brine (10 mL) and dried (Na2SO4). Evaporation of the solvent and
purification of the crude residue by silica gel column chromatography
with petroleum ether/ethyl acetate (98:2) as eluent afforded the alkyne
28 in 52% (115 mg) yield as a colorless oil. Rf 0.5 (5:95 EtOAc/
petroleum ether); [R]26D -22.9 (c 3.70, CHCl3); IR (neat) 3312, 2955,
2930, 2858, 1464, 1253 cm-1; 1H NMR (400 MHz, CDCl3) δ 4.42 (dd,
1H, J = 7.7, 2.0 Hz), 4.14 (ddd, 1H, J = 10.0, 5.7, 3.0 Hz), 4.01 (dd, 1H,
J = 7.7, 5.6 Hz), 2.49 (d, 1H, J = 2.1 Hz), 1.71-1.50 (m, 3H), 1.46 (s,
3H), 1.36 (s, 3H),1.35-1.22 (m, 9H), 0.89-0.86 (m, 12H), 0.20 (s,
3H), 0.14 (s, 3H); 13C NMR (100 MHz, CDCl3) δ 108.1, 83.6, 79.9,
77.9, 76.7, 74.2, 62.3, 31.8, 29.6, 29.3, 29.2, 28.1, 26.8, 25.8, 25.6, 22.6,
18.0, 14.1, -3.9, -4.8; HRMS (m/z) [M þ Na]þ calcd for C21H40O3Si,
391.2644; found, 391.2645.
’ ACKNOWLEDGMENT
We thank the Department of Science and Technology (DST),
New Delhi for funding. K.R.P. is a swarnajayanthi fellow of DST,
New Delhi. B.S. thanks Council of Scientific and Industrial
Research (CSIR), New Delhi for a senior research fellowship.
’ REFERENCES
(1) Yun, H.; Chou, T.-C.; Dong, H.; Tian, Y.; Li, Y-m; Danishefsky,
S. J. J. Org. Chem. 2005, 70, 10375.
(2) Ramos, F.; Takaishi, Y.; Kawazoe, K.; Osorio, C.; Duque, C.;
Acuna, R.; Fujimoto, Y.; Sato, M.; Okamoto, M.; Oshikawa, T.; Ahmed,
S. U. Phytochemistry 2006, 67, 1143.
(3) Higashibayashi, S.; Czechtizky, W.; Kobayashi, Y.; Kishi, Y. J. Am.
Chem. Soc. 2003, 125, 14379.
(4) Ghosh, S.; Pradhan, T, K. Synlett 2007, 2433.
(5) Toda, F.; Tanaka, K. J. Org. Chem. 1988, 53, 3607.
(6) For a general approach to the synthesis of γ-keto amides from
tartaric acid, see: Prasad, K. R.; Chandrakumar, A. Tetrahedron 2007,
63, 1798. For recent application of γ-keto amides derived from tartaric
acid in natural product synthesis see:(a) Prasad, K. R.; Pawar, A. B.
Synlett 2010, 1093. (b) Prasad, K. R.; Pawar, A. B. ARKIVOC 2010, 39.
Compound 29. To a precooled (0 °C) solution of the silyl ether 28
(0.15 g, 0.43 mmol) in THF (4 mL) was added TBAF (0.8 mL, 0.8
mmol, 1 M in THF), and the mixture was stirred at room temperature
for 1 h, quenched with water (5 mL), and extracted with diethyl ether
2038
dx.doi.org/10.1021/jo102201k |J. Org. Chem. 2011, 76, 2029–2039

The Journal of Organic Chemistry
ARTICLE
(c) Prasad, K. R.; Gandi, V. R.; Nidhiry, J. E.; Bhat, K. S. Synthesis
2010, 2521. (d) Prasad, K. R.; Gandi, V. R. Synlett 2009, 2593.
(e) Prasad, K. R.; Gholap, S. L. J. Org. Chem. 2008, 73, 2. (f) Prasad,
K. R.; Gholap, S. L. J. Org. Chem. 2008, 73, 2916. (g) Prasad, K. R.; Swain,
B. Tetrahedron: Asymmetry 2008, 19, 1134. (h) Prasad, K. R.;
Chandrakumar, A. J. Org. Chem. 2007, 72, 6312. (i) Prasad, K. R.;
Gholap, S. L. J. Org. Chem. 2006, 71, 3643.
(7) Minor diastereomer obtained in the reaction is separable at this
stage by column chromatography.
(8) Corey, E. J.; Fuchs, P. L. Tetrahedron Lett. 1972, 13, 3769.
(9) Yao, Z. J.; Wu, Y. L. J. Org. Chem. 1996, 60, 1170.
(10) Roth, G. J.; Liepold, B.; M€uller, S. G.; H. Bestmann, H. J.
Synthesis 2004, 59.
(11) (a) Brandsma, L. Preparative acetylenic chemistry. In Studies in
Organic Chemistry; Elsevier: Amsterdam, 1988. (b) Siemsen, P.; Living-
ston, R. C.; Diederich, F. Angew. Chem., Int. Ed. 2000, 39, 2632.
(12) In addition to the required diyne 16, a varying amount (15-
19%) of the homo coupled product of the bromoalkyne is also formed.
(13) (a) Mitsunobu, O. Synthesis 1981, 1. (b) Swamy, K. C. K.;
Kumar, N. N. B.; Balaraman, E.; Kumar, K. V. P. P. Chem. Rev. 2009,
109, 2551.
(14) Kiso, M.; Hasegawa, A. Carbohydr. Res. 1976, 52, 95.
(15) Stereochemistry of the double bond is of no consequence as it is
saturated in the next step.
2039
dx.doi.org/10.1021/jo102201k |J. Org. Chem. 2011, 76, 2029–2039