740
A.T.M. Fiori et al. / Inorganic Chemistry Communications 14 (2011) 738–740
Table 1
Antibiotic sensitive profile of bacteria against Au(I)–ibuprofen, sodium ibuprofenate and ceftriaxone.
Compounds
Results
E. coli
S. aureus
P. aeruginosa
Inhibition zone diameter (mm)
Inhibition zone diameter (mm)
Inhibition zone diameter (mm)
Au(I)–ibuprofen
Sodium ibuprofenate
Ceftriaxone
25.0 ( 0.1)
0.0
30.0 ( 0.1)
23.0 ( 0.1)
0.0
43.0 ( 0.1)
28.0 ( 0.1)
0.0
23.0 ( 0.1)
A typical microbial assay was carried out in order to evaluate the
antibacterial activity of the Au(I)–ibuprofen complex. In this study,
three referenced pathogenic bacteria (Escherichia coli ATCC 25922,
Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC
25923) were selected. The antibiogram assay was performed by the
disc diffusion method [15,16]. The sensitivity of the complex was
tested in Mueller-Hinton (MH) agar plates. The microorganisms were
transferred to separate test tubes containing 5.0 mL of sterile brain
heart infusion (BHI) medium and incubated for 18 h at 35–37 °C.
Sufficient inocula were added in new tubes until the turbidity equaled
to 0.5 McFarland (1.5×108 CFU mL−1). The bacterial inocula diluted
with BHI (McFarland standard) were uniformly spread using sterile
cotton swabs on sterile MH agar Petri dishes.
Sterile filter paper discs of 10 mm in diameter were aseptically
impregnated with 400 μg of Au(I)–ibuprofen or 400 μg of pure sodium
ibuprofenate. Discs were dried and sterilized before the experiment. All
impregnated discs were placed on the surface of the solid agar. The plates
were incubated for 18 h at 35–37 °C and examined thereafter. Clear zones
of inhibition formed around the discs were measured and the complex
sensitivity was assayed from the diameter of the inhibition zones (in
millimeters). Experiments were performed in duplicate and the results
were compared with ceftriaxone, a commercial standard antibiotic [17]. It
was found that paper discs impregnated with Au(I)–ibuprofen exhibited
inhibition zones for E. coli, P. aeruginosa and S. aureus of 25.0 mm
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Appendix A. Supplementary Material
Supplementary data to this article can be found online at
doi:10.1016/j.inoche.2011.02.024.