Heterobifunctional Trityl Derivatives as Linking Reagents for the Recovery of Nucleic Acids after Labeling and Immobilization

Article abstract of DOI:10.1016/0040-4020(95)00137-W

The synthesis of two heterobifunctional 4,4'-dimethoxytrityl derivatives carrying a N-hydroxysuccinic acid group as active ester in para 5a or meta position 5b is described.While 5a has been synthesized previously via six steps a novel and more efficient four step synthesis for both compounds is introduced here.The meta compound 5b provides for a more acid labile trityl ether linkage compared to the para derivative 5a.Using these compounds as linking agents biopolymers in particular nucleic acids can easily be recovered after labeling or immobilization by treatment with a mild acid such as 80percent aqueous acetic acid.