Synthesis and stability of S-cyanoethyl-protected 4-thiouridine and 2'-deoxy-4-thiouridine

Article abstract of DOI:10.1016/0040-4039(91)80679-Z

A convenient preparation of S-(2-cyanoethyl) protected 2'-deoxy-4-thiouridine (5a) and 4-thiouridine (5b) is presented. The S-cyanoethyl protecting group is stable to reagents commonly encountered during the phosphoramidite method of solid-phase oligonucleotide synthesis, and is readily deprotected using concentrated NH₄OH at 25°C.