Synthesis, evaluation of cytotoxic properties of promising curcumin analogues and investigation of possible molecular mechanisms

Article abstract of DOI:10.1111/cbdd.13061

Curcumin is a popular, plant-derived compound that has been extensively investigated for diverse range of biological activities. Anticancer activity against various types of cancers and high-safety profile associated with curcumin makes it very attractive. In this study, we report the synthesis and evaluation of pyrazole and click chemistry curcumin analogues for Head and Neck cancer. MTT assay against head and neck cancer cell lines CAL27 and UM-SCC-74A revealed the micromolar potency of the synthesized compounds. To determine the possible molecular mechanisms, effect of these analogues in the expression of pSTAT3, pFAK, pERK1/2 and pAKT was studied. Interestingly, compounds 2 and 5 significantly inhibited the pSTAT3 (Tyr 705) phosphorylation. As far as other compounds, they showed potent cytotoxicity against CAL27; however, these compounds did not show any activity on pSTAT3 phosphorylation at IC₅₀ concentration level. Molecular docking studies revealed the possible binding mode of pyrazole compound 2 in the SH2 domain of STAT3.

Full text of DOI:10.1111/cbdd.13061

DR. CHELLIAH SELVAM (Orcid ID : 0000-0003-1394-9349)
Article type : Research Letter
Synthesis, evaluation of cytotoxic properties of promising curcumin
analogues and investigation of possible molecular mechanisms
Brian C Jordana, Bhavna Kumarb, Ramasamy Thilagavathic, Arti Yadhavb, Pawan Kumarb and
Chelliah Selvama*
aDepartment of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Texas Southern
University, Houston, TX 77004, USA
b Department of Otolaryngology-Head and Neck Surgery; The Ohio State University Wexner Medical
Center, Columbus, OH, USA
cDepartment of Biotechnology, Faculty of Engineering, Karpagam University, Coimbatore, India
Address correspondence to Chelliah Selvam, Department of Pharmaceutical Sciences,
College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX-77004,
USA. E-mail: chelliahs@tsu.edu
Curcumin is a popular, plant-derived compound that has been extensively investigated for
diverse range of biological activities. Anti-cancer activity against various types of cancers
and high safety profile associated with curcumin makes it very attractive. In this study, we
report the synthesis and evaluation of pyrazole and click chemistry curcumin analogues for
Head and Neck cancer. MTT assay against head and neck cancer cell lines CAL27 and UM-
SCC-74A revealed the micromolar potency of the synthesized compounds. To determine
the possible molecular mechanisms, effect of these analogues in the expression of pSTAT3,
pFAK, pERK1/2 and pAKT was studied. Interestingly, compounds 2 and 5 significantly
inhibited the pSTAT3 (Tyr 705) phosphorylation. As far as other compounds, they showed
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doi: 10.1111/cbdd.13061
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potent cytotoxicity against CAL27 however these compounds didn’t show any activity on
pSTAT3 phosphorylation at IC50 concentration level. Molecular docking studies revealed the
possible binding mode of pyrazole compound 2 in the SH2 domain of STAT3.
KEYWORDS
Curcumin, Pyrazole analogue, Click-chemistry curcumin analogue, HNSCC
Curcumin, a natural polyphenol which is used as a food colorant, and herbal medicine in
Asia. Curcumin has received a lot of attention recently due to its high safety profile and
efficacy in treating various diseases (1-3).Potential anti-cancer activities associated with
curcumin makes it a promising therapeutic agent. Apart from anti-cancer activity, it has
shown anti-oxidant, anti-inflammatory, chemopreventive, chemotherapeutic, anti-
microbial, and anti-fungal activities.
Curcumin (Figure 1) is being vigorously studied for its effect against breast cancer, prostate
cancer, liver cancer, ovarian cancer, and cervical cancer (4). It demonstrated cytotoxic
effects against cancer cell lines and cytoprotective effects on non-cancer cells. One of the
important aspects of curcumin is its high safety profile of up to 12 g per day oral
administration (5).
Nevertheless, there are a number of challenges, low aqueous solubility, poor absorption,
and rapid metabolism that limits its therapeutic efficacy (6). Curcumin can exitas a
tautomeric mixture of keto and enol forms in solutions, and the enol form was found to be
responsible for the rapid degradation of the curcumin. Chakraborti, S. et al., (7) found that
the stability of curcumin was improved when the central diketone moiety of the curcumin
was replaced by isooxazole and pyrazole groups. Structural modifications of curcumin were
proven to be a meaningful approach to discover analogues with enhanced properties (8-12).
Conjugates of curcumin with β- and ƴ-cyclodextrin, liposomal and loaded nanoparticles and
nanoemulsion are some of the lipid-based colloidal system that have been employed to
enhance water solubility and improving its bioavailability (13). Recent work supports the
synergistic effect of curcumin against various cancers when administered with therapeutic
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cancer agents. Docetaxel, paclitaxel, bicalutamide, phenethyliosthiacyante, epigallocatechin
gallate, and other agents in combination with curcumin enhanced cell growth inhibition and
apoptosis (14).
Head and neck squamous cell carcinoma (HNSCC) is one of the most common types of
cancer in the U.S. It includes tumors of the mouth, nose, sinuses, salivary gland, throat, and
lymph nodes in the neck. The oral cavity and pharynx malignancy represent about 4%
(34,780) of all cancer cases, with over 9,000 deaths twice as common in men as is women.
The potential for recurrence of the malignancy is 35% within 2 years indicating its high
importance in treatment (15). The effect of curcumin to inhibit the expression of matrix
metalloproteases (MMP) in HNSCC cells was demonstrated. CCLL23, CAL27, UM-SCC1, and
UM-SCC14A head and neck cancer cells respond differently to curcumin. There is a
significant increase in cell death in CCL23 and CAL27 cell lines with 50 µmol/L of curcumin
for 24 h. Treatment of 400 µM of curcumin in a time and concentration manner resulted in
nearly 100 % cell death (16).
The activity of curcumin against head and neck squamous cell carcinoma in in-vitro and in
vivo models is reported (17). Structural modifications of curcumin serve as a meaningful
approach to discovering novel analogues to enhance the efficacy and improve its poor
pharmacokinetics. A few of the structurally modified curcumin analogous have displayed
efficacious anti-head and neck cancer activity (18).
Chakravarti et al reported the over expression of STAT3 in multiple head and neck cancers
(19). Curcumin was found to suppress the IL-6 mediated phosphorylation of STAT3. The
growth of immortalized oral mucosal epithelial cells and squamous carcinoma cells were
found to be suppressed by curcumin (20).
In the previously published studies, heterocyclic modification at the keto-enol moiety of
curcumin has been proven to be an important pharmacophore playing a pivotal role in
various biological activities including anti-oxidant, anti-Alzheimer’s, anti-androgenic, and
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cytotoxicity. According to the literature, an appropriate modification at the keto-enol
region could cause enhanced potency (21). In this work, design, synthesis and evaluation of
few semi-synthetic curcumin analogues for cytotoxic activity against HNSCC cells are
reported.
Curcumin was isolated from commercial curcumin powder by silica gel column
chromatography using CH2Cl2: MeOH: AcOH. Final product was co-evaporated with toluene
to remove traces of acetic acid to obtain pure curcumin 1. Pyrazole analogues, 2, 3, 4, 5,
and 6 were prepared (scheme 1) by treating pure curcumin (1.2 mmol) in glacial acetic acid
(9 ml) and substituted hydrazines (2.0 mmol) (22). The solution was refluxed for 8 h at
118C and monitored by thin layer chromatography (Dichloromethane: Methanol, 95:5).
The solution was evaporated under reduced pressure, co-evaporated with toluene. Crude
product was further purified by silica gel chromatography, eluting with CH2Cl2-MeOH. “Click-
chemistry” curcumin analogues 8 and 9 were prepared (scheme 2) by two step reaction. In
the first step mono-propargyl curcumin was prepared using propargyl bromide. The mono-
propargyl curcumin was treated with 2,3,4-tri-O-acetyl-β-D-xylopyranosylazide or
thioacetate propyl azide, copper sulfate and sodium ascorbate with click-chemistry reaction
protocol (23, 24).
The in-vitro cytotoxicity of the synthesized curcumin analogues (2-9) were evaluated by MTT
assay method against head and neck cancer cell lines CAL27 and UM-SCC-74A (18). Results
showed that all the compounds except compound 9 were significantly active (Figure 2). To
determine the possible molecular mechanisms, a detailed study was done. STAT3 activation
in intracellular signaling has been cited as one of the most critical steps in HNSCC
progression. Approximately 82 % of HNSCC exhibit up-regulation of STAT3 expression.
However, limited data exists on the role of semi-synthetic curcumin analogue on STAT3
pathway in head and neck cancer. To find out the mechanism of the analogues, the effects
on pSTAT3, pFAK, pERK1/2 and pAKT signaling was investigated at IC50 dose level as shown
in figure 3. Interestingly, Compounds 2 and 5 showed good activity against CAL27 cell line
(Figure 2), while inhibiting pSTAT3 (Tyr 705) phosphorylation at IC50 dose level (Figure 3).
Moreover 7 showed weak inhibitory activity on pSTAT3 phosphorylation. Compound 8
appears to be acting on pFAK and pAKT pathways. Although compounds 3, 4, 6 and 7,
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exhibited potent cytotoxicity against CAL27 and UM-SCC-74A (Figure 2), but these
compounds didn’t show any activity on pSTAT3, pFAK, PERK1/2 and pAKT signaling
pathways. Further studies are needed to support the mechanism of these compounds.
To determine the binding mode, molecular docking of one of the pSTAT3 active compounds,
2 into the SH2 domain of STAT3 was carried out using Fred (25-27) module. Omega2 (28, 29)
was used to generate conformations of the ligand. The crystal structure of STAT3β (pdb
code 1BG1) (30) was used to generate the receptor grid with the help of makereceptor (25-
27) and a hydrogen bond constraint was applied for Arg609, one of the crucial amino acid in
the phospho-tyrosine binding pocket.
The predicted binding mode of pyrazole compound 2 showed hydrogen bond formation
between the methoxy substituent and Arg609 (Figure 4, 5). There is a cation-pi interaction
between Lys 591 and the phenyl ring of pyrazole compound. Recent docking studies by
Zhang et al (31) report the interaction of these two residues in the binding of compounds
S3I-1757 and S3I-1756. The other phenyl ring has hydrophobic interaction with Pro715.
In conclusion, we synthesized curcumin analogues (compounds 2-9) and tested against head
and neck cancer cell lines CAL27 and UM-SCC-74A. Among them, compounds 5 and 8
showed potent cytotoxic activity against HNSCC cell lines. Interestingly, Two analogues, 2, 5
have significant effect on pSTAT3 phosphorylation. Interruption of pFAK and pAKT
phosphorylation signaling is shown with the compound 8. Compound 5, is the first
reported click-chemistry curcumin analogue showing good cytotoxic activity. Thus, we
investigated the cytotoxic activity of curcumin analogues and studied the possible molecular
mechanisms. Moreover, possible binding mode of compound 2 was predicted by docking
into the SH2 domain of STAT3. Overall, this study resulted in important findings and it paves
a path to further explore the design and development of more potent compounds for head
and neck cancer.
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Conflicts of interest
The authors declare that there are no conflicts of interest.
Acknowledgments
This publication was made possible, in part, by RCMI Pilot Project (Dr. Selvam Chelliah) and
molecular biology research infrastructure support from grant number 2G12MD007605 from
the NIMHD/NIH. Financial support of this research by Texas Southern University is also
gratefully acknowledged. We thank the Openeye Inc for providing the academic license to
use the docking tools.
Figure legends
Figure 1. Structure of Curcumin (1)
Scheme 1. Synthesis of Curcumin pyrazole analogues.
Scheme 2. Synthesis of Curcumin click chemistry analogues
Figure 2. Anticancer activities of Compound 2-9 against CAL27 and UM-SCC-74A cell line
Figure 3. Effect of curcumin analogues on pSTAT3, pFAK, pERK1/2 and pAKT signaling
pathways at IC50 dose level.
Figure 4. (a). Predicted binding mode of pyrazole compound 2 in the active site of STAT3-
SH2. (b). 2-D interaction diagram
Figure 5. Overlay of phosphor-tyrosine peptide (magenta) and pyrazole compound 2 (cyan).
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