Bioscience, Biotechnology and Biochemistry p. 3237 - 3248 (2008)
Update date:2022-08-03
Topics:
Uchimura, Hiromasa
Horisaki, Tadafumi
Umeda, Takashi
Noguchi, Haruko
Usami, Yusuke
Li, Li
Terada, Tohru
Nakamura, Shugo
Shimizu, Kentaro
Takemura, Tetsuo
Habe, Hiroshi
Furihata, Kazuo
Omori, Toshio
Yamane, Hisakazu
Nojiri, Hideaki
Carbazole 1,9a-dioxygenase (CARDO) consists of terminal oxygenase (CARDO-O) and electron transport components. CARDO can catalyze specific oxygenation for various substrates: angular dioxygenation for carbazole and dibenzo-p-dioxin, lateral dioxygenation for anthracene, and monooxygenation for methylene carbon of fluorene and sulfide sulfur of dibenzothiophene. To elucidate the molecular mechanism determining its unique substrate specificity, 17 CARDO-O site-directed mutants at amino acid residues I262, F275, Q282, and F329, which form the substrate-interacting wall around the iron active site by CARDO-O crystal structure, were generated and characterized. F329 replacement dramatically reduced oxygenation activity. However, several mutants produced different products from the wild-type enzyme to a large extent: I262V and Q282Y (1-hydroxycarbazole), F275W (4-hydroxyfluorene), F275A (unidentified cis-dihydrodiol of fluoranthene), and I262A and I262W (monohydroxydibenzothiophenes). These results suggest the possibility that the respective substrates bind to the active sites of CARDO-O mutants in a different orientation from that of the wild-type enzyme.
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Doi:10.1021/om400838g
(2013)Doi:10.1246/cl.130463
(2013)Doi:10.1002/jlac.199719970121
(1997)Doi:10.1021/ja409995w
(2013)Doi:10.1007/BF00531488
(1992)Doi:10.1111/j.1432-1033.1973.tb02902.x
(1973)