Bioscience, Biotechnology and Biochemistry p. 3237 - 3248 (2008)
Update date:2022-08-03
Topics:
Uchimura, Hiromasa
Horisaki, Tadafumi
Umeda, Takashi
Noguchi, Haruko
Usami, Yusuke
Li, Li
Terada, Tohru
Nakamura, Shugo
Shimizu, Kentaro
Takemura, Tetsuo
Habe, Hiroshi
Furihata, Kazuo
Omori, Toshio
Yamane, Hisakazu
Nojiri, Hideaki
Carbazole 1,9a-dioxygenase (CARDO) consists of terminal oxygenase (CARDO-O) and electron transport components. CARDO can catalyze specific oxygenation for various substrates: angular dioxygenation for carbazole and dibenzo-p-dioxin, lateral dioxygenation for anthracene, and monooxygenation for methylene carbon of fluorene and sulfide sulfur of dibenzothiophene. To elucidate the molecular mechanism determining its unique substrate specificity, 17 CARDO-O site-directed mutants at amino acid residues I262, F275, Q282, and F329, which form the substrate-interacting wall around the iron active site by CARDO-O crystal structure, were generated and characterized. F329 replacement dramatically reduced oxygenation activity. However, several mutants produced different products from the wild-type enzyme to a large extent: I262V and Q282Y (1-hydroxycarbazole), F275W (4-hydroxyfluorene), F275A (unidentified cis-dihydrodiol of fluoranthene), and I262A and I262W (monohydroxydibenzothiophenes). These results suggest the possibility that the respective substrates bind to the active sites of CARDO-O mutants in a different orientation from that of the wild-type enzyme.
View MoreShanghai Synmedia Chemical Co., Ltd
Contact:+86-21-38681880
Address:6th Floor, 11A Building, No.528 Ruiqing Road, Heqing town, Pudong new district, Shanghai China
Chemtrade International ( China )
Contact:+86-532-86893005
Address:Rm 2-501, Huaxia Zonghe Building, No. 410 JInggangshan Road, Huangdao
Nanjing Vincero International Trading Co.,Ltd
Contact:8618936897229
Address:NO.68, ZhuShan Road, JiangNing WanDa Plaza, Building E Room 1703
Wuhan Jadechem International Trade Co.,Ltd.
website:http://www.jadechem-intl.com
Contact:+86-27-83527060
Address:Room 502,Building C11,Software new city No.8,Huacheng Avenue,East Lake High-tech development zone,Wuhan,Hubei,China
Hangzhou Zhenghan Biological Technology Co., Ltd.
Contact:86-571-88781753
Address:liangzhu yuhang hangzhou city
Doi:10.1021/om400838g
(2013)Doi:10.1246/cl.130463
(2013)Doi:10.1002/jlac.199719970121
(1997)Doi:10.1021/ja409995w
(2013)Doi:10.1007/BF00531488
(1992)Doi:10.1111/j.1432-1033.1973.tb02902.x
(1973)