Oligonucleotides with (N-thymin-1-ylacetyl)1-phenylserinol in backbone: Chiral acyclic analogues that form DNA triplexes

Article abstract of DOI:10.1016/S0960-894X(97)10105-6

The synthesis of oligonucleotides containing chiral acyclic 2(R/S)-(N- thymin-1-ylacetyl)-amino-1 (R/S)-phenyl-1,3-propanediol unit in the backbone (I, R=Ar) is described. When used as third strand of a triplex with complementary natural duplex, the modified oligonucleotides form stable triplexes with triplex <=> duplex transition Tm dependent on the number, position and stereochemistry of modification.