Interaction of (2'-5') and (3'-5') linked 2-aminoadenylyl-2- aminoadenosines with polyuridylic acid

Article abstract of DOI:10.1080/15257779508009488

2'-5' and 3'-5' linked 2-aminoadenylyl-2-aminoadenosines [(2'5')n²Apn²A (1) and (3'-5')n²Apn²A (2)] were synthesized by condensation of 5'-O- monomethoxytrityl-N²,N⁶-dibenzoyl-2-aminoadenosine and N²,N⁶,2',3'-O- tetrabenzoyl-2-aminoadenosine 5'-phosphate using dicyclohexylcarbodiimide (DCC). The conformational properties of these dimers 1 and 2 were examined by UV, NMR and CD spectroscopy. The results reveal that the 2'-5'-isomer 1 takes a stacked conformation, which contains a larger base-base overlap and is room stable against thermal perturbation with respect to the 3'-5'-isomer 2. Interactions of 1 and 2 with polyuridylic acid (Poly (U)) were also examined by Tm, mixing curves, UV and CD spectra. Both the dinucleoside isomers 1 and 2 formed a complex of 1 : 2 stoichiometry with poly(U), which was much more stable than that of the corresponding ApA isomer

Full text of DOI:10.1080/15257779508009488

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Nucleosides and Nucleotides
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Interaction of (2′ -5′) and (3′ -5′) Linked
2-Aminoadenylyl-3-aminoadenosines
with Polyuridylic Acid
Masako Muraoka a , Seizo Takahashi a & Seiichi Uesugi b
a Department of Chemical and Biological Sciences, Faculty of
Science , Japan Women's University , 2-8-1 Mejirodai, Bunkyo-ku,
Tokyo, 112, Japan
b Department of Bioengineering, Faculty of Engineering , Yokohama
National University Hodogaya-ku , Yokohama, 240, Japan
Published online: 24 Sep 2006.
To cite this article: Masako Muraoka , Seizo Takahashi & Seiichi Uesugi (1995) Interaction of (2′
-5′) and (3′ -5′) Linked 2-Aminoadenylyl-3-aminoadenosines with Polyuridylic Acid, Nucleosides and
Nucleotides, 14:7, 1503-1518, DOI: 10.1080/15257779508009488
To link to this article: http://dx.doi.org/10.1080/15257779508009488
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