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ASSOCIATED CONTENT
Supporting Information
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Detailed experimental procedures for the syntheses and
characterization of formyl-lumiflavin (4), formyl-FMN (7),
amino-FMN (8) and the PFBHA derivatized oxime standards,
AFMN synthase overexpression and purification, enzymatic
assays, H/D exchange experiment, co-elution experiment,
measurement of oxygen consumption for formyl-FMN for-
mation in AFMN synthase assay, NMR and LCMS analysis.
AUTHOR INFORMATION
Corresponding Author
Note
While this paper was under review, similar findings were
reported by Professor Mack using a genetics rather than a
chemical approach to the identification of AFMN synthase.22
The papers differ in some of the details of the reconstitution
and mechanistic analysis.
ACKNOWLEDGMENT
This research was supported by the Robert A. Welch Founda-
tion (A-0034 to TPB) and by a grant from the National Insti-
tutes of Health (DK44083).
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