Chemoenzymatic syntheses of N-trifluoroacetyl-L-daunosamine, N-trifluoroacetyl-L-acosamine, N-benzoyl-D-acosamine, and N-benzoyl-D-ristosamine from an achiral precursor, methyl sorbate

Article abstract of DOI:10.1016/S0957-4166(00)00395-5

A conjugated addition of benzylamine to methyl (4R,5S)-4,5-(isopropylidenedioxy)-(2E)-hexenoate 8a followed by lactonization under acidic condition proceeds to the formal total syntheses of L-daunosamine 4 and L-acosamine 2. On the other hand, direct conjugated addition of benzylamine to methyl (4S,5S)-4,5-epoxy-(2E)-hexenoate 5 and the subsequent intramolecular nucleophilic attack by the ester carbonyl group on the epoxy ring of the substrates leads to the formal total syntheses of D-acosamine 2 and D-ristosamine 1. Copyright (C) 2000 Elsevier Science Ltd.

Full text of DOI:10.1016/S0957-4166(00)00395-5

TETRAHEDRON:
ASYMMETRY
Pergamon
Tetrahedron: Asymmetry 11 (2000) 4137–4151
Chemoenzymatic syntheses of N-trifluoroacetyl-
-daunosamine, N-trifluoroacetyl-
-acosamine, N-benzoyl- -acosamine, and N-benzoyl-
L
L
D
D-ristosamine from an achiral precursor, methyl sorbate
Chikako Saotome, Machiko Ono and Hiroyuki Akita*
School of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
Received 28 August 2000; accepted 28 September 2000
Abstract
A conjugated addition of benzylamine to methyl (4R,5S)-4,5-(isopropylidenedioxy)-(2E)-hexenoate 8a
followed by lactonization under acidic condition proceeds to the formal total syntheses of -daunosamine
4 and -acosamine 2. On the other hand, direct conjugated addition of benzylamine to methyl
(4S,5S)-4,5-epoxy-(2E)-hexenoate 5 and the subsequent intramolecular nucleophilic attack by the ester
carbonyl group on the epoxy ring of the substrates leads to the formal total syntheses of -acosamine 2
and -ristosamine 1. © 2000 Elsevier Science Ltd. All rights reserved.
L
L
D
D
1. Introduction
Three of the four diastereomers 1–4 of 3-amino-2,3,6-trideoxy- -hexose are the naturally
L
occurring amino sugars1 (Scheme 1). The ribo-isomer,
-ristosamine 1, is a component of the
L
Scheme 1.
* Corresponding author. E-mail: akita@phar.toho-u.ac.jp
0957-4166/00/$ - see front matter © 2000 Elsevier Science Ltd. All rights reserved.
PII: S0957-4166(00)00395-5

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water-soluble glycoprotein ristomycin, which is a member of the vancomycin group of antibi-
otics.1 Acosamine with the arabino-configuration have been found in both its
- and -form.
-Acosame 2 was isolated from the antibiotic actinoidine and -acosamine 2 was obtained from
the basic antibiotic N-acetylsporaciridine.1 While the xylo-isomer, 3-epi-
-daunosamine 3, so far
has not been found in nature, the lyxo-isomer, -daunosamine 4, is the glycosidic component of
a number of important anthracycline antibiotics that exhibit impressive activity against a broad
range of solid tumors and soft tissue sarcomas.1 Changing
-daunosamine of adriamycin with its
4-epimer, -acosamine 2 was reported to suppress the cardiotoxicity while retaining the
anti-tumor activity.2 Therefore, considerable interest has been shown in developing syntheses of
enantiomerically pure -daunosamine 4 and its analogues in order to provide sufficient material
for pharmaceutical structure–activity studies.3 Of several syntheses of
-daunosamine 4, almost
all of the chiral syntheses are based on conversion of natural carbohydrates such as -mannose,
-rhamnose and
-glucose.1 Approaches from non-carbohydrate precursors have also been
reported; however, the known syntheses of 4 seem to be rather impractical.4 We wish to report
formal total syntheses of -ristosamine 1, -acosamine 2, -acosamine 2, and -daunosamine 4,
L
D
L
D
L
L
L
L
L
L
D
L
D
D
L
D
L
starting from an achiral precursor, methyl sorbate, and employing enzymatic chiral induction
and diastereoselective conjugated addition of benzylamine to the a,b-unsaturated ester.
2. Results and discussion
We reported the syntheses of the enantiomerically pure stereoisomers of trans-(4,5)-epoxy-
2(E)-hexenoates (4S,5S)-5 and (4R,5R)-5 based on a chemoenzymatic method from an achiral
precursor, methyl sorbate (Scheme 2).5 For the syntheses of the target molecules from (4S,5S)-5,
two synthetic routes are considered. One is the 1,4-addition of benzylamine to the a,b-unsatu-
rated ester after epoxy ring opening of (4S,5S)-5 by oxygen nucleophiles such as benzyl alcohol.
The other is the direct 1,4-addition of benzylamine to the (4S,5S)-5 and the subsequent
regioselective cleavage of the epoxy ring by an intramolecular nucleophilic attack by the ester
carbonyl group. At first, as a preliminary experiment of the former case, 1,4-addition of
benzylamine to the olefinic moiety of the racemic 8a–e derived from the reported benzyl ether
( )-65 was carried out. Treatment of ( )-6 with AlCl3 in the presence of m-xylene5 gave a diol
( )-7, which was treated with 2,2-dimethoxypropane, cyclohexanone, cyclopentanone, and
Scheme 2. (a) AlCl3, m-xylene/CH2Cl2; (b) Me2C(OMe)2/CSA for ( )-8a, cyclohexanone/p-TsOH·H2O for ( )-8b,
cyclopentanone/p-TsOH·H2O for ( )-8c, benzaldehyde/p-TsOH·H2O for ( )-8d,e

C. Saotome et al. / Tetrahedron: Asymmetry 11 (2000) 4137–4151
4139
benzaldehyde in the presence of acid to afford the acetonide ( )-8a (89%), ketals ( )-8b
(45%) and ( )-8c (85%), benzylidene acetals ( )-8d (26%) and ( )-8e (57%), respectively. The
stereochemistry of the more polar benzylidene acetal ( )-8e was confirmed by difference
nuclear Overhauser effect (NOE) spectra as shown in Fig. 1. The reaction of ( )-8a–e with
benzylamine (2 equivalents) in the absence of solvent at room temperature afforded the
1,4-addition products, ( )-3,4-syn-9a–e and ( )-3,4-anti-10a–e, along with a small amount of
starting materials 8a–e as shown in Table 1. In the case of entries 1 and 2, the ratio of
3,4-syn-9 and 3,4-anti-10 were found to be 13.4:1 and 12.6:1, respectively. In order to
determine the stereochemistry of the main product ( )-3,4-syn-9a, ( )-9a was converted into
the known compound ( )-11. Hydrogenolysis of ( )-9a gave the 3-amino ester ( )-12
(Scheme 3). Acetylation of ( )-12 followed by treatment with 80% AcOH aqueous solution
gave the ( )-arabino-3-acetoamino-g-lactone 13, which was converted into the known ( )-ara-
bino-3-acetoamino-5-acetoxy-g-lactone 11.6 The 3,4-syn stereochemistry of other ( )-9b–e was
confirmed by the fact that treatment of ( )-9b–e with 2,2-dimethoxypropane in the presence
of camphorsulfonic acid (CSA) afforded the above-mentioned ( )-9a. The stereochemistry of
the minor product ( )-10a was confirmed to be 3,4-anti because optically active 10a was
chemically correlated to the known compound mentioned later in this text. From these
conversion experiments, the relative configuration of the conjugated addition products ( )-9a–
e was determined to be 3,4-syn and thence that of ( )-10a–e was confirmed to be 3,4-anti.
The 3,4-syn-selective addition of a nucleophile to 8a is explainable by a Felkin–Anh model7
as depicted in Fig. 2.
Figure 1.
Table 1
Entry
(9)-9a–e:(9)-10a–e
(9)-9a–e (%)
(9)-10a–e (%)
1
2
3
4
5
R1=R2=Me
R1=R2=ꢀ(CH2)5ꢀ (9)-8b
R1=R2=ꢀ(CH2)4ꢀ (9)-8c
R1=Ph, R2=H
R1=H, R2=Ph
(9)-8a
13.4:1
12.6:1
5.7:1
5.1:1
4:1
(9)-9a (67)
(9)-9b (63)
(9)-9c (57)
(9)-9d (67)
(9)-9e (52)
(9)-10a (5)
(9)-10b (5)
(9)-10c (10)
(9)-10d (13)
(9)-10e (13)
(9)-8d
(9)-8e

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Scheme 3. (a) H2/Pd(OH)2ꢀC; (b) (1) Ac2O/pyridine, (2) 80% AcOH aq.; (c) Ac2O/AcONa, reflux; (d) 40 equiv.
Me2C(OMe)2/2 equiv. CSA/acetone, 90°C
Figure 2.
Then, the formal syntheses of
L
-acosamine 2 and -daunosamine 4 from (4S,5S)-epoxy ester
L
5 are described. The reaction of (4S,5S)-5 with benzyl alcohol in the presence of BF3·Et2O
afforded (4R,5S)-65 {[h]D −71.6 (c=1.24, CHCl3)} as the main product, whose NMR spectra
were identical with those of the reported5 ( )-6 (Scheme 4). Treatment of (4R,5S)-6 with AlCl3
in the presence of m-xylene5 gave a diol (4R,5S)-7 {[h]D +16.7 (c=0.86, CHCl3)}, which was
subjected to acetonide formation to provide acetonide (4R,5S)-8a {[h]D +0.49 (c=3.67,
CHCl3)}. The reaction of (4R,5S)-8a with benzylamine (2 equivalents) in the absence of solvent
at room temperature afforded the 1,4-addition products, (3S,4R,5S)-9a {[h]D +15.9 (c=1.92,
CHCl3)} and (3R,4R,5S)-10a {[h]D −9.81 (c=0.43, CHCl3)}. Hydrogenolysis of (+)-9a followed
by treatment of the 3-amino ester (3S,4R,5S)-12 {[h]D −4.8 (c=3.21, CHCl3)} with benzoyl
chloride gave the 3-benzoylamino ester (3S,4R,5S)-14 {[h]D +9.3 (c=2.84, CHCl3)}. Cleavage of
the acetonide and the subsequent lactonization of 14 in aqueous 80% AcOH at reflux afforded
1
the g-lactone (3S,4R,5S)-15. Physical data {mp 139°C, [h]D −47.3 (c=0.77, EtOH), IR and H
NMR} of the present g-lactone 15 were identical with those {mp 155°C, [h]D −43.2 (c=1.1,
1
EtOH), IR and H NMR} of the reported (3S,4R,5S)-15.4 Therefore, the stereochemistries of
(+)-9a and (−)-10a were determined to be (3S,4R,5S)-configuration and (3R,4R,5S)-configura-
tion, respectively. As conversions of (3S,4R,5S)-15 into N-trifluoroacetyl-
L
-daunosamine 16 and
N-trifluoroacetyl-
L
-acosamine 17 have been reported,4 chiral syntheses of the above-mentioned
two amino sugar derivatives from an achiral precursor, methyl sorbate, could be achieved.
Next, the reaction of (4S,5S)-5 with benzylamine (4 equivalents) at 40°C afforded the
1,4-addition products, (3R,4S,5S)-18 {[h]D −18.7 (c=0.77, CHCl3)} and (3S,4S,5S)-19 {[h]D
−21.1 (c=0.6, CHCl3)} (Scheme 5). In order to determine the stereochemistry of the main
product (−)-18, (−)-18 was treated with CF3SO3H in CH2Cl2 at −20°C to give the d-lactone 20
{[h]D −49.2 (c=0.47, CHCl3)} and g-lactone 21 {[h]D −51.5 (c=0.71, CHCl3)}. For the purpose
of comparison, the standard samples, d-lactone (3S,4R,5S)-20 {[h]D +51.9 (c=0.4, CHCl3)} and
g-lactone (3S,4R,5S)-21 {[h]D +45.3 (c=0.23, CHCl3)}, were obtained by the treatment of the

C. Saotome et al. / Tetrahedron: Asymmetry 11 (2000) 4137–4151
4141
Scheme 4. (a) BnOH, BF3·Et2O/CH2Cl2, −20°C; (b) AlCl3, m-xylene/CH2Cl2, 0°C; (c) (MeO)2CMe2, p-TsOH·H2O/
acetone, 0°C; (d) BnNH2; (e) H2, 20% Pd(OH)2/MeOH; (f) BzCl/pyridine; (g) 80% AcOH, reflux
above-mentioned (3S,4R,5S)-9a with camphorsulfonic acid (CSA) in MeOH. The two d-lactones
were found to be enantiomeric because of identical spectrometric characteristics (IR and NMR)
except for the sign of [h]D of each enantiomer. Meanwhile, the two g-lactones of (3R,4S,5R)-21
and the standard sample (3S,4R,5S)-21 were also found to be enantiomeric. Therefore, the
stereochemistry of (−)-18 was determined to be (3R,4S,5S). The stereochemistry of the minor
product (−)-19 was also determined to be (3S,4S,5S), because physical data {[h]D +38.4
(c=0.63, CHCl3)} of (+)-g-lactone 22 derived from (−)-19 were consistent with those {[h]D −37.2
(c=0.3, CHCl3)} of (−)-g-lactone (3R,4R,5S)-22 derived from the above-mentioned (3R,4R,5S)-
10a except for the sign of [h]D of each enantiomer. In the case of lactonization of (3,4)-syn 18,
an intramolecular nucleophilic attack by an ester carbonyl group in the C5 position results in the
formation of the d-lactone 20. Under these reaction conditions, the d-lactone 20 comes to
equilibrium with the g-lactone 21. Meanwhile, in the case of lactonization of (3,4)-anti 19, an
intramolecular nucleophilic attack by an ester carbonyl group in the C5 position causes
predominantly the formation of the d-lactone, which was soon converted to the g-lactone 22.
Hydrogenolysis of (3R,4S,5R)-20 thus obtained followed by treatment with benzoyl chloride
gave a mixture (63% yield) of d-lactone 23 and g-lactone 15, which was reduced with
diisobutylaluminum hydride (DIBAL) to the N-benzoyl- -acosamine (3R,4S,5R)-24 {[h]D +13.1
D
(c=0.6, EtOH), mp 216–217°C}. The physical data (1H and 13C NMR) of the present 24 were
identical with those (1H and 13C NMR) of the reported (3R,4S,5R)-24.8 The (3S,4S,5R)-22 was
also converted into the N-benzoyl-
D
-ristosamine (3S,4S,5R)-25 via (3S,4S,5R)-26 {[h]D −46.9
(c=0.78, THF} by the same way as for the conversion of 18 to 24. The (3S,4S,5R)-25 {[h]D

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C. Saotome et al. / Tetrahedron: Asymmetry 11 (2000) 4137–4151
Scheme 5. (a) BnNH2; (b) CF3SO3H/CH2Cl2, −20°C; (c) CSA/MeOH; (d) (1) H2, 20% Pd(OH)2ꢀC/2 M HCl, (2)
BzCl/pyridine; (e) HAl(i-Bu)2/THF
+28.0 (c=0.23, EtOH), mp 131–133°C} thus obtained was consistent with the reported
N-benzoyl-
-ristosamine (3R,4R,5S)-259 {[h]D −12 (c=1, EtOH), mp 130–132°C} except for the
L
sign of [h] of each enantiomer.
In conclusion, the syntheses of
L
-amino sugars such as
L
-daunosamine 4 and -acosamine 2
L
and
D
-amino sugars such as
D
-acosamine 2 and
D
-ristosamine 1 were found to be distinguish-
able by changing the addition order of nucleophile against enantiomerically pure (4S,5S)-epoxy-
(2E)-hexenoate 5. Moreover, (4R,5R)-5 could be applied in the above-mentioned reaction as a
starting material and the syntheses of
acosamine 2 and -amino sugars such as
D
-amino sugars such as
D
-daunosamine 4 and
D
-
L
L
-acosamine 2 and -ristosamine 1 could be achieved.
L

C. Saotome et al. / Tetrahedron: Asymmetry 11 (2000) 4137–4151
4143
3. Experimental
3.1. General
All melting points were measured on a Yanaco MP-3S micro melting point apparatus and are
1
uncorrected. H and 13C NMR spectra were recorded on JEOL AL 400 spectrometer in CDCl3.
Carbon substitution degrees were established by DEPT pulse sequence. The fast-atom-bombard-
ment mass spectra (FAB MS) were obtained with JEOL JMS-DX 303 spectrometer. IR spectra
were recorded a JASCO FT/IR-300 spectrometer. Optical rotations were measured with a
JASCO DIP-370 digital polarimeter. All evaporations were performed under reduced pressure.
For column chromatography, silica gel (Kieselgel 60) was employed.
3.2. Methyl (4R*,5S*)-4,5-dihydroxy-2(E)-hexenoate ( )-7
To a well-stirred solution of AlCl3 (4.26 g, 32 mmol) in CH2Cl2 (50 ml) at 0°C was added a
solution of ( )-6 (1.6 g, 6.4 mmol) in m-xylene (10 ml) and the whole mixture was stirred for 15
min at the same temperature. The reaction mixture was poured on ice and extracted with ether.
The ether layer was washed with saturated brine and dried over MgSO4. The ether layer was
evaporated to give a crude residue, which was chromatographed on silica gel (50 g, n-hex-
ane:AcOEt=1:2) to give ( )-7 (819 mg, 80%) as a colorless oil. IR (neat): 3410, 1709 cm−1;
NMR: l 1.16 (3H, d, J=7 Hz), 2.75, 3.12 (each 1H, br. s), 3.75 (3H, s), 3.96 (1H, br.), 4.31 (1H,
br.), 6.13 (1H, dd, J=2, 16 Hz), 6.96 (1H, dd, J=5, 16 Hz). FAB MS m/z: 161 (M++1).
3.3. Preparation of ketals ( )-8a–c from ( )-7, and acetals ( )-8d,e from ( )-7
(i) A solution of ( )-7 (0.73 g, 4.4 mmol), CSA (0.1 g, 0.43 mmol), and 2,2-dimethoxypropane
(9.58 g, 92 mmol) in acetone (5 ml) was stirred for 30 min. The reaction mixture was diluted
with 7% aqueous NaHCO3 and extracted with ether. The ether layer was washed with saturated
brine and dried over MgSO4. The ether layer was evaporated to give a crude residue, which was
chromatographed on silica gel (30 g, n-hexane:AcOEt=19:1) to give ( )-8a (812 mg, 89%) as a
colorless oil. IR (neat): 1728 cm−1; NMR: l 1.16 (3H, d, J=7 Hz), 1.38, 1.51 (each 3H, s), 3.76
(3H, s), 4.43 (1H, dq, J=7, 7 Hz), 4.66 (1H, dt, J=2, 7 Hz), 6.09 (1H, dd, J=2, 16 Hz), 6.84
(1H, dd, J=6, 16 Hz). Anal. found: C, 59.50; H, 8.23. Calcd for C10H16O4: C, 59.98; H, 8.05%.
FAB MS m/z: 201 (M++1). (ii) A solution of ( )-7 (5.8 g, 36.2 mmol), p-TsOH·H2O (0.7 g, 3.68
mmol), and cyclohexanone (3.92 g, 40 mmol) in benzene (30 ml) was refluxed for 1 h by means
of a Dean–Stark apparatus. The reaction mixture was diluted with saturated brine and extracted
with ether. The ether layer was dried over MgSO4 and evaporated to give a crude residue, which
was chromatographed on silica gel (200 g) to afford ( )-8b (3.915 g, 45%) as a colorless oil from
n-hexane:AcOEt=19:1 eluate and starting material ( )-7 (1.16 g, 20% recovery) from n-hex-
ane:AcOEt=1:1 eluate. ( )-8b: IR (neat): 1728 cm−1; NMR: l 1.16 (3H, d, J=7 Hz), 1.37–1.45,
1.56–1.73 (10H, m), 3.75 (3H, s), 4.42 (1H, dq, J=6, 6 Hz), 4.65 (1H, dt, J=1.5, 6 Hz), 6.10
(1H, dd, J=1.5, 16 Hz), 6.84 (1H, dd, J=6, 16 Hz). Anal. found: C, 65.31; H, 8.56. Calcd for
C13H20O4: C, 64.98; H, 8.39%. FAB MS m/z: 241 (M++1). (iii) A solution of ( )-7 (0.69 g, 4.3
mmol), p-TsOH·H2O (0.08 g, 0.42 mmol), and cyclopentanone (0.44 g, 5.2 mmol) in benzene (20
ml) was refluxed for 1 h by means of a Dean–Stark apparatus. The reaction mixture was worked
up in the same way as for (ii) to afford ( )-8c (0.83 g, 85%) as a colorless oil. IR (neat): 1727

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C. Saotome et al. / Tetrahedron: Asymmetry 11 (2000) 4137–4151
cm−1; NMR: l 1.17 (3H, d, J=7 Hz), 1.68–1.80, 1.88–2.00 (8H, m), 3.75 (3H, s), 4.31 (1H, dq,
J=6, 6 Hz), 4.58 (1H, dt, J=1.5, 6 Hz), 6.08 (1H, dd, J=1.5, 16 Hz), 6.84 (1H, dd, J=6, 16
Hz). Anal. found: C, 63.35; H, 8.15. Calcd for C12H18O4: C, 63.70; H, 8.02%. FAB MS m/z: 227
(M++1). (iv) A solution of ( )-7 (0.58 g, 3.6 mmol), p-TsOH·H2O (0.07 g, 0.37 mmol), and
benzaldehyde (0.45 g, 4.2 mmol) in benzene (10 ml) was refluxed for 1 h by means of a
Dean–Stark apparatus. The reaction mixture was worked up in the same way as for (ii) to afford
the less polar ( )-8d (0.233 g, 26%) as a colorless oil and the more polar ( )-8e (0.512 g, 57%)
as a colorless oil. ( )-8d: IR (neat): 1724 cm−1; NMR: l 1.24 (3H, d, J=6 Hz), 3.77 (3H, s), 4.44
(1H, dq, J=6, 6 Hz), 4.81 (1H, dt, J=2, 6 Hz), 6.20 (1H, s), 6.20 (1H, dd, J=2, 16 Hz), 6.91
(1H, dd, J=6, 16 Hz), 7.33–7.47 (5H, m). Anal. found: C, 67.26; H, 6.50. Calcd for C14H16O4:
C, 67.73; H, 6.50%. FAB MS m/z: 249 (M++1). ( )-8e: IR (neat): 1726 cm−1; NMR: l 1.28 (3H,
d, J=6 Hz), 3.75 (3H, s), 4.46 (1H, dq, J=6, 6 Hz), 4.75 (1H, dt, J=1.5, 6 Hz), 5.85 (1H, s),
6.12 (1H, dd, J=1.5, 16 Hz), 6.84 (1H, dd, J=6, 16 Hz), 7.36–7.53 (5H, m). Anal. found: C,
68.02; H, 6.64. Calcd for C14H16O4: C, 67.73; H, 6.50%. FAB MS m/z: 249 (M++1).
3.4. The reactions of ketals ( )-8a–c and benzylamine, and acetals ( )-8d,e and benzylamine
(i) A mixture of ( )-8a (0.67 g, 3.3 mmol) and BnNH2 (0.72 g, 6.7 mmol) was kept standing
for 2 days at 30°C. The reaction mixture was directly chromatographed on silica gel (30 g) to
afford starting material ( )-8a (0.107 g, 16% recovery) from n-hexane:AcOEt=19:1 eluate,
( )-10a (0.051 g, 5%) as a colorless oil, and ( )-9a (0.689 g, 67%) as a colorless oil from
n-hexane:AcOEt=19:1 eluate in that order. ( )-9a: IR (neat): 3339, 1737 cm−1; NMR: l 1.26
(3H, d, J=7 Hz), 1.31, 1.45 (each 3H, s), 2.50 (2H, d, J=6 Hz), 3.18 (1H, dd, J=6, 12 Hz), 3.69
(3H, s), 3.78, 3.88 (each 1H, d, J=13 Hz), 4.10 (1H, t, J=6 Hz), 4.31 (1H, dq, J=6, 6 Hz),
7.22–7.36 (5H, m). FAB MS m/z: 308 (M++1). ( )-10a: IR (neat): 3330, 1735 cm−1; NMR: l
1.24 (3H, d, J=7 Hz), 1.31, 1.41 (each 3H, s), 2.62 (1H, dd, J=6, 16 Hz), 2.75 (1H, dd, J=4,
16 Hz), 3.13 (1H, ddd, J=4, 6, 8 Hz), 3.69 (3H, s), 3.71, 3.85 (each 1H, d, J=12 Hz), 4.02 (1H,
dd, J=6, 8 Hz), 4.38 (1H, dq, J=7, 7 Hz), 7.22–7.30 (5H, m). Anal. found: C, 66.22; H, 8.28;
N, 4.53. Calcd for C17H25NO4: C, 66.42; H, 8.20; N, 4.56%. FAB MS m/z: 308 (M++1). (ii) A
mixture of ( )-8b (0.49 g, 2 mmol) and BnNH2 (0.44 g, 4.1 mmol) was kept standing for 2 days
at 30°C. The reaction mixture was worked up in the same way as for (i) to give starting material
( )-8b (0.088 g, 18% recovery), ( )-10b (0.035 g, 5%) as a colorless oil, and ( )-9b (0.446 g, 63%)
as a colorless oil. ( )-9b: IR (neat): 3338, 1738 cm−1; NMR: l 1.23 (3H, d, J=6 Hz), 1.33–1.66
(10H, m), 2.45 (1H, dd, J=6, 15 Hz), 2.49 (1H, dd, J=5, 15 Hz), 3.18 (1H, dt, J=5, 7 Hz), 3.69
(3H, s), 3.81, 3.90 (each 1H, d, J=13 Hz), 4.09 (1H, dd, J=6, 7 Hz), 4.29 (1H, dq, J=6, 6 Hz),
7.24–7.36 (5H, m). Anal. found: C, 68.86; H, 8.67; N, 3.92. Calcd for C20H29NO4: C, 69.13; H,
8.41; N, 4.03%. FAB MS m/z: 348 (M++1). ( )-10b: IR (neat): 3333, 1734 cm−1; NMR: l 1.21
(3H, d, J=7 Hz), 1.33–1.60 (10H, m), 2.64 (1H, dd, J=6, 16 Hz), 2.75 (1H, dd, J=4, 16 Hz),
3.13 (1H, dt, J=4, 6 Hz), 3.69 (3H, s), 3.70, 3.85 (each 1H, d, J=13 Hz), 3.98 (1H, dd, J=6,
8 Hz), 4.36 (1H, dq, J=6, 6 Hz), 7.21–7.32 (5H, m). Anal. found: C, 69.06; H, 8.51; N, 4.01.
Calcd for C20H29NO4: C, 69.13; H, 8.41; N, 4.03%. FAB MS m/z: 348 (M++1). (iii) A mixture
of ( )-8c (0.43 g, 1.9 mmol) and BnNH2 (0.4 g, 3.7 mmol) was kept standing for 2 days at 30°C.
The reaction mixture was worked up in the same way as for (i) to give starting material ( )-8c
(0.138 g, 32% recovery), ( )-10c (0.063 g, 10%) as a colorless oil, and ( )-9c (0.361 g, 57%) as
a colorless oil. ( )-9c: IR (neat): 3350, 1737 cm−1; NMR: l 1.25 (3H, d, J=6 Hz), 1.62–1.73,
1.82–1.89 (8H, m), 2.51 (2H, d, J=6 Hz), 3.17 (1H, dt, J=6, 6 Hz), 3.68 (3H, s), 3.78, 3.88 (each

C. Saotome et al. / Tetrahedron: Asymmetry 11 (2000) 4137–4151
4145
1H, d, J=13 Hz), 4.00 (1H, t, J=6 Hz), 4.22 (1H, dq, J=6, 6 Hz), 7.22–7.35 (5H, m). Anal.
found: C, 68.23; H, 8.53; N, 4.14. Calcd for C19H27NO4: C, 68.44; H, 8.16; N, 4.20%. FAB MS
m/z: 334 (M++1). ( )-10c: IR (neat): 3330, 1735 cm−1; NMR: l 1.23 (3H, d, J=7 Hz), 1.63–1.71,
1.82–1.85 (8H, m), 2.58 (1H, dd, J=7, 16 Hz), 2.72 (1H, dd, J=4, 16 Hz), 3.12 (1H, dt, J=4,
7 Hz), 3.68 (3H, s), 3.72, 3.84 (each 1H, d, J=13 Hz), 3.95 (1H, dd, J=6, 7 Hz), 4.28 (1H, dq,
J=6, 6 Hz), 7.21–7.32 (5H, m). Anal. found: C, 68.29; H, 8.65; N, 4.07. Calcd for C19H27NO4:
C, 68.44; H, 8.16; N, 4.20%. FAB MS m/z: 334 (M++1). (iv) A mixture of ( )-8d (0.15 g, 0.6
mmol) and BnNH2 (0.13 g, 1.2 mmol) was kept standing for 2 days at 30°C. The reaction
mixture was worked up in the same way as for (i) to give starting material ( )-8d (0.015 g, 10%
recovery), ( )-10d (0.028 g, 13%) as a colorless oil, and ( )-9d (0.144 g, 67%) as a colorless oil.
( )-9d: IR (neat): 3344, 1735 cm−1; NMR: l 1.34 (3H, d, J=7 Hz), 2.53 (1H, dd, J=6, 13 Hz),
2.57 (1H, dd, J=6, 13 Hz), 3.26 (1H, dt, J=6, 6 Hz), 3.68 (3H, s), 3.80, 3.90 (each 1H, d, J=13
Hz), 4.19 (1H, t, J=6 Hz), 4.41 (1H, dq, J=6, 6 Hz), 6.16 (1H, s), 7.22–7.44 (10H, m). Anal.
found: C, 70.44; H, 7.16; N, 3.90. Calcd for C21H25NO4: C, 70.96; H, 7.09; N, 3.94%. FAB MS
m/z: 356 (M++1). ( )-10d: IR (neat): 3328, 1732 cm−1; NMR: l 1.32 (3H, d, J=6 Hz), 2.69 (1H,
dd, J=6, 16 Hz), 2.81 (1H, dd, J=4, 16 Hz), 3.23 (1H, ddd, J=4, 6, 9 Hz), 3.67 (3H, s), 3.72,
3.87 (each 1H, d, J=12 Hz), 4.06 (1H, dd, J=6, 9 Hz), 4.51 (1H, dq, J=6, 6 Hz), 6.05 (1H, s),
7.25–7.45 (10H, m). Anal. found: C, 70.45; H, 7.17; N, 3.69. Calcd for C21H25NO4: C, 70.96; H,
7.09; N, 3.94%. FAB MS m/z: 356 (M++1). (v) A mixture of ( )-8e (0.15 g, 0.6 mmol) and
BnNH2 (0.13 g, 1.2 mmol) was kept standing for 2 days at 30°C. The reaction mixture was
worked up in the same way as for (i) to give starting material ( )-8e (0.024 g, 16% recovery),
( )-10e (0.028 g, 13%) as a colorless oil, and ( )-9e (0.112 g, 52%) as a colorless oil. ( )-9e: IR
(neat): 3342, 1734 cm−1; NMR: l 1.42 (3H, d, J=6 Hz), 2.57 (2H, d, J=6 Hz), 3.27 (1H, dt,
J=6, 6 Hz), 3.67 (3H, s), 3.76, 3.88 (each 1H, d, J=12 Hz), 4.19 (1H, t, J=6 Hz), 4.38 (1H,
dq, J=6, 6 Hz), 5.79 (1H, s), 7.20–7.33, 7.47–7.50 (10H, m). Anal. found: C, 70.96; H, 7.23; N,
3.78. Calcd for C21H25NO4: C, 70.96; H, 7.09; N, 3.94%. FAB MS m/z: 356 (M++1). ( )-10e: IR
(neat): 3332, 1732 cm−1; NMR: l 1.36 (3H, d, J=7 Hz), 2.65 (1H, dd, J=5, 16 Hz), 2.77 (1H,
dd, J=4, 16 Hz), 3.25 (1H, ddd, J=4, 5, 8 Hz), 3.62 (3H, s), 3.73, 3.78 (each 1H, d, J=13 Hz),
4.13 (1H, dd, J=7, 8 Hz), 4.46 (1H, dq, J=7, 7 Hz), 5.75 (1H, s), 7.22–7.40, 7.45–7.47 (10H,
m). Anal. found: C, 70.57; H, 7.18; N, 3.87. Calcd for C21H25NO4: C, 70.96; H, 7.09; N, 3.94%.
FAB MS m/z: 356 (M++1).
3.5. Conversion of ( )-9a into ( )-arabino-3-acetoamino-5-acetoxy-2,3,6-trideoxyaldonic acid
k-lactone 11
(i) A solution of ( )-9a (0.5 g, 1.63 mmol) in MeOH (2 ml) was hydrogenated over 10%
Pd(OH)2–C (50 mg) at ambient temperature and pressure. After hydrogen absorption had
ceased, the catalyst was filtered off and the filtrate was evaporated to give ( )-12 (0.319 g, 90%)
as a homogeneous oil. ( )-12: IR (neat): 1720 cm−1; NMR: l 1.28 (3H, d, J=6 Hz), 1.35, 1.48
(each 3H, s), 1.68 (2H, br. s), 2.35 (1H, dd, J=9, 16 Hz), 2.43 (1H, dd, J=4, 16 Hz), 3.28–3.33
(1H, m), 3.71 (3H, s), 3.87 (1H, dd, J=6, 6 Hz), 4.30 (1H, dq, J=6, 6 Hz). Anal. found: C,
54.83; H, 8.92; N, 6.25. Calcd for C10H19NO4: C, 55.28; H, 8.82; N, 6.45%. (ii) A solution of
( )-12 (0.5 g, 2.3 mmol) and Ac2O (1.664 g 16.3 mmol) in pyridine (1 ml) was stirred for 3 days
at room temperature. The reaction mixture was worked up by the usual procedure to give the
corresponding acetate (0.417 g, 70%). A solution of the above-mentioned acetate (0.202 g, 0.78
mmol) in 80% aqueous AcOH (2 ml) was refluxed for 5 h. The reaction mixture was evaporated

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to afford a residue, which was chromatographed on silica gel (10 g, n-hexane:AcOEt=1:1) to
give ( )-13 (0.09 g, 62%) as a colorless oil. ( )-13: IR (CHCl3): 1760, 1650 cm−1; NMR: l 1.32
(3H, d, J=6 Hz), 2.07 (3H, s), 2.55 (1H, d, J=17 Hz), 2.96 (1H, dd, J=7, 17 Hz), 3.90 (1H,
dq, J=6, 8 Hz), 4.17 (1H, dd, J=4, 8 Hz), 4.51 (1H, br. s), 4.77 (1H, ddd, J=4, 6, 7 Hz), 7.48
(1H, d, J=6 Hz). Anal. found: C, 51.58; H, 7.11; N, 7.20. Calcd for C8H13NO4: C, 51.33; H,
7.00; N, 7.48%. (iii) A solution of ( )-13 (0.054 g, 0.29 mmol) and AcONa (0.045 g, 0.54 mmol)
in Ac2O (1 ml) was stirred for 2 h at 120°C. The reaction mixture was worked up by the usual
1
procedure to give ( )-11 (0.023 g, 34%). Spectral data (IR and H NMR) of ( )-11 were
consistent with the reported data.6
3.6. Conversion of ( )-9b–e into ( ) 9a
(i) A solution of ( )-9b (0.1 g, 0.29 mmol), CSA (0.13 g, 0.56 mmol) and 2,2-dimeth-
oxypropane (1.2 g, 11.5 mmol) in acetone (20 ml) was stirred for 12 h at 90°C. The reaction
mixture was worked up in the same way as for the preparation of ( )-9a to give ( )-9a (0.054
g, 61%). NMR data of the present ( )-9a were identical with those of authentic ( )-9a. (ii) By
applying method (i), ( )-9c (0.09 g, 0.27 mmol) was converted into 9a (0.051 g, 61%). (iii) By
applying method (i), ( )-9d (0.11 g, 0.31 mmol) was converted into 9a (0.061 g, 64%). (iv) By
applying method (i), ( )-9e (0.06 g, 0.17 mmol) was converted into 9a (0.021 g, 38%).
3.7. Methyl (4R,5S)-4,5-(isopropylidenedioxy)-2(E)-hexenoate 8a
(i) To a solution of (4S,5S)-5 (3.0 g, 21.1 mmol), BnOH (4.55 g, 42.1 mmol) in CH2Cl2 (30
ml) at −20°C was added BF3·Et2O (2.5 ml, 19.7 mmol) and the whole mixture was stirred for 3
h at −20°C. The reaction mixture was diluted with saturated brine and extracted with ether. The
ether layer was dried over MgSO4 and evaporated to give a residue, which was chro-
matographed on silica gel (100 g, n-hexane:AcOEt=5:1) to give (4R,5S)-6 (3.169 g, 60%) as a
1
colorless oil. [h]2D3 −71.6 (c=1.24, CHCl3); IR and H NMR data of (4R,5S)-6 were identical
with those of the reported ( )-6.5 Anal. found: C, 67.07; H, 7.50. Calcd for C14H18O4: C, 67.18;
H, 7.25%. (ii) To a well-stirred solution of AlCl3 (3.17 g, 23.7 mmol) in CH2Cl2 (20 ml) at 0°C
was added a solution of (4R,5S)-6 (1.19 g, 4.7 mmol) in m-xylene (5 ml) and the whole mixture
was stirred for 30 min at the same temperature. The reaction mixture was worked up in the same
way as for the preparation of ( )-7 to afford (4R,5S)-7 (0.594 g, 78%). [h]2D6 +16.7 (c=0.86,
1
CHCl3); IR and H NMR data of (4R,5S)-7 were identical with those of the above-mentioned
( )-7. FAB MS m/z: 161 (M++1). (iii) A solution of (4R,5S)-7 (0.6 g, 3.7 mmol), p-TsOH (0.1
g, 0.53 mmol), and 2,2-dimethoxypropane (7.95 g, 75 mmol) in acetone (5 ml) was stirred for 30
min. The reaction mixture was worked up in the same way as for the preparation of ( )-8a to
afford (4R,5S)-8a (0.577 g, 77%). [h]2D2 +0.49 (c=3.67, CHCl3); IR and H NMR data of
1
(4R,5S)-8a were identical with those of the above-mentioned ( )-8a. Anal. found: C, 59.53; H,
8.27. Calcd for C10H16O4: C, 59.98; H, 8.05%.
3.8. Methyl (3S,4R,5S)-3-benzylamino-4,5-(isopropylidenedioxy)hexanoate 9a and methyl
(3R,4R,5S)-3-benzylamino-4,5-(isopropylidenedioxy)hexanoate 10a
A mixture of (4R,5S)-8a (0.57 g, 2.8 mmol) and BnNH2 (0.61 g, 5.7 mmol) was kept standing
for 2 days at 30°C. The reaction mixture was directly chromatographed on silica gel (30 g) to

C. Saotome et al. / Tetrahedron: Asymmetry 11 (2000) 4137–4151
4147
afford starting material (4R,5S)-8a (0.034 g, 6% recovery) from n-hexane:AcOEt=19:1 eluate,
(3R,4R,5S)-10a (0.079 g, 9%) as a colorless oil, and (3S,4R,5S)-9a (0.639 g, 73%) as a colorless
oil from n-hexane:AcOEt=19:1 eluate in that order. (3S,4R,5S)-9a: [h]2D1 +15.9 (c=1.92,
1
CHCl3); IR and H NMR data of (3S,4R,5S)-9a were identical with those of the above-men-
tioned ( )-9a. Anal. found: C, 66.16; H, 8.46; N, 4.65. Calcd for C17H25NO4: C, 66.42; H, 8.20;
N, 4.56%. FAB MS m/z: 308 (M++1). (3R,4R,5S)-10a: [h]D23 −9.8 (c=0.43, CHCl3); IR and H
1
NMR data of (3S,4R,5S)-10a were identical with those of the above-mentioned ( )-10a. Anal.
found: C, 66.22; H, 8.28; N, 4.53. Calcd for C17H25NO4: C, 66.42; H, 8.20; N, 4.56%. FAB MS
m/z: 308 (M++1).
3.9. Conversion of methyl (3S,4R,5S)-3-benzylamino-4,5-(isopropylidenedioxy)hexanoate 9a into
(3S,4R,5S)-arabino-3-benzoylamino-2,3,6-trideoxyaldonic acid k-lactone 15
(i) A solution of (3S,4R,5S)-9a (0.48 g, 1.6 mmol) in MeOH (5 ml) was hydrogenated over
20% Pd(OH)2–C (50 mg) at ordinary temperature and pressure. After hydrogen absorption had
ceased, the catalyst was filtered off and the filtrate was evaporated to give (3S,4R,5S)-12 (0.319
1
g, 94%) as a homogeneous oil. (3S,4R,5S)-12: [h]2D3 −4.8 (c=3.21, CHCl3); IR and H NMR
data of (3S,4R,5S)-12 were identical with those of the above-mentioned ( )-12. (ii) A solution
of (3S,4R,5S)-12 (0.32 g, 1.5 mmol) and PhCOCl (0.31 g 2.2 mmol) in pyridine (1 ml) was
stirred for 3 days at room temperature. The reaction mixture was diluted with 7% aqueous
NaHCO3 and extracted with ether. The organic layer was washed with saturated brine, dried
over MgSO4 and evaporated. The residue was chromatographed on silica gel (10 g, n-hex-
ane:AcOEt=1:1) to give (3S,4R,5S)-14 (0.383 g, 81%) as a colorless oil. (3S,4R,5S)-14: [h]D21
+9.3 (c=2.84, CHCl3); IR (CHCl3): 1760, 1650 cm−1; NMR: l 1.33 (3H, d, J=6 Hz), 1.40, 1.57
(each 3H, s), 2.69 (1H, dd, J=8, 16 Hz), 2.77 (1H, dd, J=5, 16 Hz), 3.69 (3H, s), 4.33 (1H, dd,
J=2, 7 Hz), 4.47 (1H, dq, J=6, 6 Hz), 4.50–4.54 (1H, m), 6.69 (1H, d, J=8 Hz), 7.42–7.53 (3H,
m), 7.75–7.78 (2H, m). Anal. found: C, 63.97; H, 7.29; N, 4.38. Calcd for C17H23NO5: C, 63.53;
H, 7.21; N, 4.36%. FAB MS m/z: 322 (M++1). (iii) A solution of (3S,4R,5S)-14 (0.28 g, 0.89
mmol) in 80% aqueous AcOH (5 ml) was refluxed for 4.5 h. The reaction mixture was condensed
under reduced pressure and the residue was chromatographed on silica gel (10 g, n-hex-
ane:AcOEt=1:1) to give (3S,4R,5S)-15 (0.165 g, 76%) as a colorless oil. Recrystallization of
(3S,4R,5S)-15 from CH2Cl2/Et2O gave colorless prisms: mp 159°C; [h]1D9 −47.3 (c=0.77, EtOH);
IR (CHCl3): 3600, 1740, 1640 cm−1; NMR: l 1.31 (3H, d, J=6 Hz), 2.75 (1H, dd, J=1, 18 Hz),
3.05 (1H, dd, J=7, 18 Hz), 4.08 (1H, dq, J=6, 6 Hz), 4.31 (1H, dd, J=4, 6 Hz), 4.99–5.04 (1H,
m), 7.44 (2H, t, J=7 Hz), 7.54 (1H, t, J=7 Hz), 7.86 (2H, d, J=7 Hz), 8.12 (1H, br. s). Anal.
found: C, 62.35; H, 6.18; N, 5.49. Calcd for C13H15NO4: C, 62.64; H, 6.07; N, 5.62%. FAB MS
1
m/z: 249 (M++1). Physical data (mp, IR and H NMR) of (3S,4R,5S)-15 were identical with
those {mp 155°C, [h]D −43.2 (c=1.1, EtOH)} of the reported (3S,4R,5S)-15.4
3.10. Methyl (3R,4S,5S)-3-benzylamino-4,5-epoxyhexanoate 18 and methyl
(3S,4S,5S)-3-benzylamino-4,5-epoxyhexanoate 19
A mixture of (4S,5S)-5 (1.17 g, 8.2 mmol) and BnNH2 (3.53 g, 33 mmol) was kept standing
for 2 days at 40°C. The reaction mixture was directly chromatographed on silica gel (120 g,
n-hexane:AcOEt=2:1) to afford (3R,4S,5S)-18 (1.087 g, 53%) as a colorless oil and (3S,4S,5S)-
19 (0.656 g, 32%) as a colorless oil in eluate order. (3R,4S,5S)-18: [h]3D0 −18.7 (c=0.77, CHCl3);

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IR (neat): 3329, 1736 cm−1; NMR: l 1.30 (3H, d, J=5 Hz), 2.50, 2.58 (1H, dd, J=8, 16 Hz),
2.76 (1H, dd, J=2, 6 Hz), 2.89 (1H, q, J=7 Hz), 2.89 (1H, dq, J=2, 5 Hz), 3.68 (3H, s), 3.84,
3.90 (each 1H, d, J=13 Hz), 7.21–7.34 (5H, m). Anal. found: C, 67.57; H, 7.91; N, 5.57. Calcd
for C14H19NO3: C, 67.45; H, 7.68; N, 5.62%. FAB MS m/z: 250 (M++1). (3S,4S,5S)-19: [h]2D9
−21.1 (c=0.6, CHCl3); IR (neat): 3334, 1734 cm−1; NMR: l 1.24 (3H, d, J=5 Hz), 2.53 (1H, dd,
J=8, 16 Hz), 2.62 (1H, dd, J=2, 7 Hz), 2.69 (1H, dd, J=4, 16 Hz), 2.75 (1H, ddd, J=4, 7, 8
Hz), 2.83 (1H, dq, J=2, 5 Hz), 3.68 (3H, s), 3.81, 3.86 (each 1H, d, J=13 Hz), 7.21–7.34 (5H,
m). Anal. found: C, 67.56; H, 7.86; N, 5.50. Calcd for C14H19NO3: C, 67.45; H, 7.68; N, 5.62%.
FAB MS m/z: 250 (M++1).
3.11. (3R,4S,5R)-arabino-3-Benzylamino-trideoxyaldonic acid l-lactone 20 and
(3R,4S,5R)-arabino-3-benzylamino-trideoxyaldonic acid k-lactone 21
A solution of (3R,4S,5S)-18 (0.42 g, 1.7 mmol) and CF3SO3H (0.3 ml, 3.4 mmol) in CH2Cl2
(40 ml) was stirred for 4 h at −20°C. The reaction mixture was diluted with 7% aqueous
NaHCO3 and extracted with ether. The organic layer was washed with saturated brine, dried
over MgSO4 and evaporated. The residue was chromatographed on silica gel (20 g, n-hex-
ane:AcOEt=1:1) to give (3R,4S,5R)-21 (0.071 g, 18%) from n-hexane:AcOEt=2:1 eluate as a
colorless oil and (3R,4S,5R)-20 (0.253 g, 64%) from n-hexane:AcOEt=1:1 eluate as a colorless
oil. Recrystallization of the latter fraction from n-hexane/Et2O gave colorless crystal
(3R,4S,5R)-20. (3R,4S,5R)-20: mp 94–95°C; [h]2D8 −49.2 (c=0.47, CHCl3); IR (CHCl3): 3423,
3162, 1734 cm−1; NMR: l 1.45 (3H, d, J=6 Hz), 2.36 (1H, dd, J=9, 17 Hz), 2.93 (1H, dt, J=6,
9 Hz), 3.03 (1H, dd, J=6, 17 Hz), 3.28 (1H, t, J=9 Hz), 3.72, 3.88 (each 1H, d, J=14 Hz), 4.12
(1H, dq, J=6, 9 Hz), 7.26–7.36 (5H, m). FAB MS m/z: 236 (M++1). HRMS (FAB+ MS, matrix;
m-nitrobenzyl alcohol (NBA)): calcd for C13H18NO3 (M++1) 236.1287; found 236.1284.
(3R,4S,5R)-21: [h]2D5 −51.5 (c=0.71, CHCl3); IR (neat): 3414, 3318, 1778 cm−1; NMR: l 1.33
(3H, d, J=6 Hz), 2.62 (1H, dd, J=4, 18 Hz), 2.71 (1H, dd, J=7, 18 Hz), 3.70 (1H, ddd, J=4,
6, 7 Hz), 3.70, 3.88 (each 1H, d, J=13 Hz), 3.99 (1H, dq, J=6, 8 Hz), 4.15 (1H, dd, J=6, 8 Hz),
7.26–7.37 (5H, m). FAB MS m/z: 236 (M++1). HRMS (FAB+ MS, matrix; NBA): calcd for
C13H18NO3 (M++1) 236.1287; found 236.1307.
3.12. (3S,4R,5S)-arabino-3-Benzylamino-trideoxyaldonic acid l-lactone 20 and
(3S,4R,5S)-arabino-3-benzylamino-trideoxyaldonic acid k-lactone 21
A solution of (3S,4R,5S)-9a (0.16 g, 0.52 mmol) and CSA (0.24 g, 1.0 mmol) in MeOH (2 ml)
was stirred for 2 days at room temperature. The reaction mixture was worked up in the same
way as described in Section 3.11 to give (3S,4R,5S)-21 (0.022 g, 18%) and (3S,4R,5S)-20 (0.076
g, 62%). (3S,4R,5S)-20: [h]2D9 +51.9 (c=0.40, CHCl3); H NMR spectra of (3S,4R,5S)-20 were
1
identical with those of (3R,4S,5R)-20. FAB MS m/z: 236 (M++1). (3S,4R,5S)-21: [h]D24 +45.3
(c=0.23, CHCl3); 1H NMR spectra of (3S,4R,5S)-21 were identical with those of (3R,4S,5R)-21.
FAB MS m/z: 236 (M++1).
3.13. (3S,4S,5R)-ribo-3-Benzylamino-trideoxyaldonic acid k-lactone 22
A solution of (3S,4S,5S)-19 (0.37 g, 1.5 mmol) and CF3SO3H (0.27 ml, 3.0 mmol) in CH2Cl2
(40 ml) was stirred for 4 h at −20°C. The reaction mixture was diluted with 7% aqueous

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NaHCO3 and extracted with ether. The organic layer was washed with saturated brine, dried
over MgSO4 and evaporated. The residue was chromatographed on silica gel (20 g, n-hex-
ane:AcOEt=1:1) to give (3S,4S,5R)-22 (0.297 g, 85%) as a colorless oil. (3S,4S,5R)-22: [h]D28
+38.4 (c=0.63, CHCl3); IR (KBr): 3405, 3127, 1765 cm−1; NMR: l 1.27 (3H, d, J=6 Hz), 2.37
(1H, dd, J=5, 18 Hz), 2.83 (1H, dd, J=8, 18 Hz), 3.58 (1H, dt, J=5, 8 Hz), 3.75, 3.81 (1H, d,
J=13 Hz), 3.97 (1H, dq, J=6, 6 Hz), 4.06 (1H, t, J=5 Hz), 7.27–7.37 (5H, m). Anal. found:
C, 66.03; H, 7.52; N, 5.81. Calcd for C13H17NO3: C, 66.36; H, 7.28; N, 5.95%. FAB MS m/z:
236 (M++1).
3.14. (3R,4R,5S)-ribo-3-Benzylamino-trideoxyaldonic acid k-lactone 22
A solution of (3R,4R,5S)-10a (0.04 g, 0.16 mmol) and CSA (0.06 g, 0.26 mmol) in MeOH (1
ml) was stirred for 12 h at room temperature. The reaction mixture was diluted with 7% aqueous
NaHCO3 and extracted with ether. The organic layer was washed with saturated brine, dried
over MgSO4 and evaporated. The residue was chromatographed on silica gel (10 g, n-hex-
ane:AcOEt=1:1) to give (3R,4R,5S)-22 (0.034 g, 90%) as a colorless oil. [h]2D3 −37.2 (c=0.3,
1
CHCl3); H NMR spectra of (3R,4R,5S)-22 were identical with those of (3S,4S,5R)-22. FAB
MS m/z: 236 (M++1).
3.15. Conversion of (3R,4S,5R)-20 into N-benzoyl- -acosamine 24
D
(i) A solution of (3R,4S,5R)-20 (0.34 g, 1.4 mmol) in 2 M HCl (4 ml) was hydrogenated over
20% Pd(OH)2–C (30 mg) at ordinary temperature and pressure. After hydrogen absorption had
ceased, the catalyst was filtered off and the filtrate was treated with 7% aqueous NaHCO3 and
evaporated. A mixture of the residue and PhCOCl (0.4 g, 3.4 mmol) in pyridine (3 ml) was
stirred for 12 h at room temperature. The reaction mixture was diluted with H2O and extracted
with AcOEt. The organic layer was dried over MgSO4 and evaporated to give a residue, which
was chromatographed on silica gel (10 g, n-hexane:AcOEt=2:1) to give a mixture (0.227 g, 63%)
of (3R,4S,5R)-23 and (3R,4S,5R)-15. (ii) To a solution of the above-mentioned mixture (0.14 g,
0.56 mmol) in THF (3 ml) was added a 1.5 M solution of DIBAL (0.75 ml, 1.1 mmol) in toluene
at −78°C, and the whole mixture was stirred for 1 h. MeOH (1.5 ml) was added to the reaction
mixture at −50°C and the whole mixture was filtered off with the aid of Celite. The precipitate
was washed with acetone. The washing was combined with the filtrate and the combined organic
layer was evaporated to give a residue, which was chromatographed on silica gel (10 g,
n-hexane:AcOEt=1:1) to give N-benzoyl- -acosamine 24 (0.069 g, 49%). Recrystallization of 24
D
from benzene afforded colorless crystal 24. mp 216–217°C; [h]2D3 +13.1 (c=0.6, EtOH); Anal.
found: C, 62.49; H, 6.96; N, 5.14. Calcd for C13H17NO4: C, 62.14; H, 6.82; N, 5.57%. IR (KBr):
1
3370, 3284, 1631, 1544 cm−1; a-form: H NMR (DMSO-d6): l 1.15 (3H, d, J=6 Hz), 1.66 (1H,
dt, J=3, 13 Hz), 1.84 (1H, ddd, J=1, 5, 13 Hz), 3.11 (1H, dt, J=6, 10 Hz), 3.81 (1H, dq, J=6,
9 Hz), 4.25 (1H, dddd, J=5, 8, 9, 13 Hz), 4.85 (1H, d, J=6 Hz), 5.10 (1H, ddd, J=1, 3, 4 Hz),
6.15 (1H, d, J=4 Hz), 7.43–7.54, 7.84–7.87 (5H, m), 8.10 (1H, d, J=8 Hz). 13C NMR
1
(DMSO-d6): l 18.3, 36.9, 48.3, 67.6, 74.2, 89.8. b-form: H NMR: l 1.20 (3H, d, J=6 Hz), 1.51
(1H, dt, J=10, 13 Hz), 1.94 (1H, ddd, J=2, 5, 13 Hz), 3.08 (1H, ddd, J=6, 9, 10 Hz), 3.28 (1H,
dq, J=6, 10 Hz), 3.94 (1H, dddd, J=8, 9, 10, 13 Hz), 4.71 (1H, ddd, J=2, 6, 10 Hz), 4.85 (1H,
d, J=6 Hz), 6.51 (1H, d, J=4 Hz), 7.43–7.54, 7.84–7.87 (5H, m), 8.23 (1H, d, J=8 Hz). 13C
1
NMR (DMSO-d6): l 18.3, 38.91, 51.5, 72.8, 73.4, 93.5. Physical data (mp, IR and H NMR) of
24 were identical with those (1H NMR) of the reported 24.8

4150
C. Saotome et al. / Tetrahedron: Asymmetry 11 (2000) 4137–4151
3.16. Conversion of (3S,4S,5R)-22 into N-benzoyl- -ristosamine 25
D
(i) A solution of (3S,4S,5R)-22 (0.27 g, 1.14 mmol) in 2 M HCl (3 ml) was hydrogenated over
20% Pd(OH)2–C (30 mg) at ordinary temperature and pressure. After hydrogen absorption had
ceased, the catalyst was filtered off and the filtrate was treated with 7% aqueous NaHCO3 and
evaporated. A mixture of the residue and PhCOCl (0.36 g, 2.5 mmol) in pyridine (4 ml) was
stirred for 12 h at room temperature. The reaction mixture was diluted with H2O and extracted
with AcOEt. The organic layer was dried over MgSO4 and evaporated to give a residue, which
was chromatographed on silica gel (10 g, n-hexane:AcOEt=1:1) to give (3S,4S,5R)-26 (0.137 g,
48%). Recrystallization of 26 from AcOEt afforded colorless crystal 26. mp 149°C; [h]2D6 −46.9
(c=0.78, THF); IR (KBr): 3340, 1758, 1645, 1543 cm−1; NMR: l 1.39 (3H, d, J=7 Hz), 2.64
(1H, dd, J=5, 19 Hz), 4.04 (1H, dq, J=5, 7 Hz), 4.31 (1H, dd, J=4, 5 Hz), 4.83–4.89 (1H, m),
7.19 (1H, br. d, J=7 Hz), 7.41–7.56, 7.79–7.82 (5H, m). Anal. found: C, 60.90; H, 6.37; N, 5.78.
Calcd for C13H15NO4·0.5H2O: C, 60.46; H, 6.24; N, 5.42%. FAB MS m/z: 250 (M++1). (ii) To
a solution of (3S,4S,5R)-26 (0.08 g, 0.32 mmol) in THF (2 ml) was added a 1.5 M solution of
DIBAL (0.45 ml, 0.68 mmol) in toluene at −78°C, and the whole mixture was stirred for 1 h.
MeOH (1.5 ml) was added to the reaction mixture at −50°C and the whole mixture was filtered
off with the aid of Celite. The precipitate was washed with acetone. The washing was combined
with the filtrate and the combined organic layer was evaporated to give a residue, which was
chromatographed on silica gel (10 g, n-hexane:AcOEt=1:1) to give N-benzoyl- -ristosamine 25
D
(0.037 g, 46%). Recrystallization of 25 from AcOEt afforded colorless crystal 25. mp 131–133°C;
[h]2D5 +28.0 (c=0.23, EtOH); Anal. found: C, 61.70; H, 7.11; N, 5.11. Calcd for C13H17NO4: C,
1
62.14; H, 6.82; N, 5.57%. IR (KBr): 3391, 3291, 1638, 1541 cm−1; a-form: H NMR (DMSO-d6):
l 1.07 (3H, d, J=6 Hz), 1.79 (1H, dd, J=5, 13 Hz), 2.30 (1H, dd, J=9, 13 Hz), 3.65 (1H, dq,
J=5, 6 Hz), 3.91 (1H, dt, J=4, 5 Hz), 4.44 (1H, dddd, J=5, 5, 8, 9 Hz), 4.63 (1H, m), 5.43 (1H,
ddd, J=2, 5, 5 Hz), 6.32 (1H, d, J=5 Hz), 7.45–7.55, 7.75–7.87 (5H, m), 8.37 (1H, d, J=8 Hz).
1
13C NMR (DMSO-d6): l 17.9, 33.8, 46.8, 65.0, 71.3, 89.9. b-form: H NMR (DMSO-d6): l 1.10
(3H, d, J=6 Hz), 2.06 (2H, m), 3.70 (2H, m), 4.68 (1H, m), 5.42 (1H, m), 6.32 (1H, d, J=5 Hz),
7.45–7.55, 7.75–7.87 (5H, m), 8.55 (1H, d, J=8 Hz). 13C NMR (DMSO-d6): l 18.6, 48.4, 69.6,
1
70.9, 91.2. Physical data (mp, IR and H NMR) of 25 were identical with those (mp 130–132°C)
of the reported 25.9
Acknowledgements
The authors are grateful to Professor Yoshiteru Ida, School of Pharmaceutical Science,
Showa University for measuring the high-resolution mass spectra (FAB MS).
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