Enzymatic α(2-3)sialylation of non-natural type-I (Lewis(c)) disaccharides with recombinant sialyl-transferase

Article abstract of DOI:10.1016/S0960-894X(97)10201-3

Recombinant α(2-3)sialyl-transferase from rat liver is used to sialylate a series of type-I (Lewis(c)) disaccharides on a preparative scale. The enzyme tolerates a broad array of N-acetyl replacements of the N-glucosamine subunit ranging from small and large lipophilic groups to charged and heterocyclic amides.