Journal of Organic Chemistry (2019)
Update date:2022-07-30
Topics:
Ghodke, Pratibha P.
Bommisetti, Praneeth
Nair, Deepak T.
Pradeepkumar
We report the synthesis of N2-aryl (benzyl, naphthyl, anthracenyl, and pyrenyl)-deoxyguanosine (dG) modified phosphoramidite building blocks and the corresponding damaged DNAs. Primer extension studies using E. coli Pol IV, a translesion polymerase, demonstrate that translesion synthesis (TLS) across these N2-dG adducts is error free. However, the efficiency of TLS activity decreases with increase in the steric bulkiness of the adducts. Molecular dynamics simulations of damaged DNA-Pol IV complexes reveal the van der Waals interactions between key amino acid residues (Phe13, Ile31, Gly32, Gly33, Ser42, Pro73, Gly74, Phe76, and Tyr79) of the enzyme and adduct that help to accommodate the bulky damages in a hydrophobic pocket to facilitate TLS. Overall, the results presented here provide insights into the TLS across N2-aryl-dG damaged DNAs by Pol IV.
View MoreShanghai Yurui Biotechnology(Anyang) Pharmaceutical Co., Ltd
Contact:+86-0372-3662335 +86-0372-3661988
Address:hanling industrial park anyang
Xiamen Huasing Chemicals Co.Ltd.
Contact:0086-592-6228397
Address:NO.24, Xinglin North 2nd Road,Jimei district
Contact:+86-20-62802632;62802633
Address:Room 330 GIGCAS Building,No.511 Kehua Street,Tianhe District
Shandong Xinke Petrochemical Co., Ltd.
Contact:+86-546-7277016
Address:Gudao Industrial Park, Hekou District, Dongying, Shandong Province, China
Shenyang Xingzhenghe Chemical Co., Ltd.
Contact:024-23509232
Address:No. 33, Naner Road, Heping Dist.
Doi:10.1021/jm010888h
(2001)Doi:10.1002/jps.2600530623
(1964)Doi:10.1016/S0040-4020(01)96868-1
(1968)Doi:10.1007/BF01169876
()Doi:10.1016/S0040-4020(00)00902-9
(2000)Doi:10.1021/jm9803572
(1998)