Catalytic activity of carboxypeptidase B and of carboxypeptidase Y with anisylazoformyl substrates

Article abstract of DOI:10.1016/S0960-894X(98)00715-X

Anisylazoformyllysine (CH₃OC₆H₄-N=N-CO-Lys-OH) is rapidly hydrolyzed at the acyl-lysine linkage by the zinc-enzyme porcine carboxypeptidase B. The catalytic reaction is readily monitored spectrophotometrically by disappearance of the intense absorption (348.5 nm, ε 18400) of the azo chromophore, which chemically fragments after substrate cleavage. Carboxypeptidase Y has no activity toward this type of substrate.