A pyrene SECO-pseudonucleoside in constructing interaction-sensitive fluorescent DNA probes

Article abstract of DOI:10.1080/07328319908044632

Based on 4-(1-pyrenyl)-1,3-butanediol, a pyrene seco-pseudonucleoside, we prepared a nonnucleotide phosphoramidite reagent and a pyrene-modified LCAA-CPG-support and employed them in solid-phase synthesis of pyrene-labeled oligonucleotides with varying numbers and positions of the pseudonucleoside residues. Changes in the fluorescence intensity and the spectral curve shape upon hybridization were observed that depended on the position(s) of the modified unit(s) in the probe.

Full text of DOI:10.1080/07328319908044632

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Nucleosides and Nucleotides
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A Pyrene Seco-Pseudonucleoside in
Constructing Interaction-Sensitive
Fluorescent DNA Probes
Vladimir A. Korshun a , Konstantin V. Balakin a , Tatyana S.
Proskurina a , Ilya I. Mikhalev a , Andrei D. Malakhov a & Yuri A.
Berlin a
a Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian
Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP-7 Moscow,
117871, Russia Fax: E-mail:
Available online: 04 Oct 2006
To cite this article: Vladimir A. Korshun, Konstantin V. Balakin, Tatyana S. Proskurina, Ilya I.
Mikhalev, Andrei D. Malakhov & Yuri A. Berlin (1999): A Pyrene Seco-Pseudonucleoside in Constructing
Interaction-Sensitive Fluorescent DNA Probes, Nucleosides and Nucleotides, 18:11-12, 2661-2676
To link to this article: http://dx.doi.org/10.1080/07328319908044632
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