Bioorganic Chemistry p. 198 - 210 (2001)
Update date:2022-08-05
Topics:
Lan, Tao
McLaughlin, Larry W.
Two nucleoside derivatives containing the base analogues 3-deazaadenine and 3-methyl-2-pyridone have been prepared as analogues of dA and dT, respectively. After conversion into the appropriately protected phosphoramidites, DNA sequences were prepared with site-specifically placed analogues. When present in a duplex DNA sequence, the analogues result in the deletion of one or both of the hydrogen bonding functional groups (the N3-nitrogen of dA and the O2-carbonyl of dT) present in the minor groove. Binding by two ligands, 4′,6-diamidine-2-phenyl indole (DAPI) and Hoechst 33258 in the minor groove has been probed using a variety of DNA sequences. These sequences contain a d(GAATTC)2 core with analogue nucleosides substituted for one or more of the dA and dT residues. DAPI bound strongly to any sequence that contained both O2-carbonyls of the central two dT residues. The presence of a dc3A residue did in some cases enhance binding. With one of the central O2-carbonyls deleted, the binding was noticeably reduced, and with both absent, no significant binding could be detected. Similar although less dramatic results were observed with Hoechst 33258 binding to analogue sequences.
View MoreShanghai Norky Pharmaceutical Co., LTD.
website:http://www.norkypharm.com
Contact:86-21-61075300
Address:1165 Jiangning Road, Office 1502, Shanghai, China
Weifang Arylchem Chemical Co., LTD
Contact:86-536-5217866
Address:Development Zone, Shouguang, Shandong Province
Hangzhou yi lu biont technology Co., LTD
Contact:+86-571-88152630
Address:Hangzhoushi HuafengRoad Yicheng3Building1001room.
Contact:+86-579-85206992
Address:No 451 chouzhou north road ,room 1106 int'l business center , yiwu ,china
Contact:86-571-85586718
Address:join hands science park,NO.4028 nanhuan road,hanghzou ,zhejiang,china
Doi:10.1055/s-1981-29473
(1981)Doi:10.1016/S0040-4020(00)00346-X
(2000)Doi:10.1016/S0957-4166(00)00132-4
(2000)Doi:10.1016/S0040-4039(00)00608-0
(2000)Doi:10.1039/b000326n
(2000)Doi:10.1016/j.tet.2018.10.068
(2019)