In vitro oxidation of indoleacetic acid by crude enzyme from rice husk - An aspect of preharvest sprouting

Article abstract of DOI:10.3987/COM-97-S(N)12

Indoleacetic acid was oxidized under oxygen atmosphere by the crude enzyme solution prepared by blending rice husk in buffer solution of pH 7.3. The major oxidation products (3, 4, and 5), characterized by physical and chemical methods, showed little effects on germination and growth of lettuce seeds while IAA inhibited completely the germination at 5 ppm. This evidence suggests that the amounts of IAA in embryos decreases due to the oxidation by water soluble oxidation enzyme existing in rice husk. The decrease of IAA may be related, in some aspect, to dormancy breaking and also preharvest sprouting of rice seeds. The synthesis and optical resolution of 2 was also achieved.