Molecules 2005, 10
1177
This product was obtained in 37 % yield from 5a and 4. The product was pure according to HPLC
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and H-NMR analysis. H-NMR (D2O): δ 0.87 (t, J = 6.8 Hz, 3H), 1.29-1.50 (m, 18H), 2.00-2.06 (m,
2H), 2.97-3.01 (m, 2H), 3.43 (dd, J = 7.4 Hz, J = 10.7 Hz, 1H), 3.55 (dd, J = 3.4 Hz, J = 10.7 Hz, 1H),
3.59-3.71 (m, 2H), 3.83 (m, 1H) ppm; IR (KBr): 3444, 2958, 2915, 2850, 1469, 1444, 1420, 1401,
1360, 1322, 1280, 1234, 1194, 1161, 1124, 1067, 1051, 905, 878, 797, 721, 629 cm-1.
Sodium 12-hydroxy-4,7,10-trioxa-1-docosanesulfonate (7c).
This product was obtained in 80 % yield from 4 and 5c. The product was pure according to HPLC
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and H-NMR analysis. H-NMR (D2O): δ 0.87 (t, J = 6.6 Hz, 3H), 1.29-1.45 (m, 18H), 2.02 (m, 2H),
2.96 (m, 2H), 3.42 (dd, J = 7.5 Hz, J = 10.5 Hz, 1H), 3.53 (dd, J = 3.2 Hz, J = 10.5 Hz, 1H), 3.63-3.69
(m, 10H), 3.81 (m, 1H) ppm; IR (KBr): 3378, 2928, 2913, 2849, 1469, 1421, 1356, 1330, 1266, 1245,
1201, 1130, 1069, 908, 892, 798, 734, 721, 628 cm-1.
Sodium 6-decyl-9-hydroxy-4,7-dioxa-1-decanesulfonate (10a).
Hydroxysulfonate 7a (1.50 g; 4.33 mmol) and sodium hydride (0.26 g; 10.8 mmol) were dissolved
in freshly distilled THF (20 mL) under a N2-atmosphere. The reaction was heated to 55°C for 1 hour.
Propylene sulfate (3, 0.90 g, 6.49 mmol) in dry THF (10 mL) was added, and the reaction was stirred
at 55°C overnight. The reaction mixture was cooled in an ice bath and quenched with ethanol (2 mL).
The solvent was removed under reduced pressure. The product was dissolved in 2 M hydrochloric acid
(50 mL) and refluxed for 4 hours. The reaction was cooled in an ice bath and neutralized with NaOH
(aq.). The solution was extracted continuously with isoamyl alcohol for 3.5 hours and the isoamyl
alcohol was removed under reduced pressure. The product was loaded onto a column of silica gel and
eluted with acetone (2 x 250 mL, frac. 1-2), acetone-ethanol 9:1 (450 mL, frac. 3-14), acetone-ethanol
1:1 (450 mL, frac. 15-26) and pure ethanol (450 mL, frac. 27-38). The fractions were analyzed by TLC
using ethanol as eluent. Fractions 10 to 34 contained the product. The solvents were removed under
reduced pressure and the product was dried in vacuo, yielding 1.34 g (77 %) of a waxy solid. The
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purity of isolated 10a was 85 % according to H-NMR analysis, with a 15 % content of the non-
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alkylated substrate 7a. H-NMR (D2O): δ 0.76 (t, J = 6.7 Hz, 3H), 1.05 (dd, J = 6.4 Hz, J = 2.3 Hz,
2.6H), 1.18-1.41 (m, 18H), 1.91 (m, 2H), 2.87 (m, 2H), 3.29-3.65 (m, 7.6H), 3.86 (m, 0.9H) ppm; IR
(NaCl): 3334, 2954, 2922, 2852, 1466, 1190, 1110, 1054, 617 cm-1.
Sodium 9-decyl-12-hydroxy-4,7,10-trioxa-1-tridecanesulfonate (10b).
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Obtained in 86 % yield (4.95 g) from 7b and 3. The purity was 97 % according to H-NMR (it
contained 3 % of the hydroxysulfonate 7b). 1H-NMR (D2O): δ 0.88 (t, J = 6.4 Hz, 3H), 1.14 (m, 2.9H),
1.29-1.50 (m, 18H), 2.01 (m, 2H), 2.95 (m, 2H), 3.38-3.69 (m, 11H), 3.93 (m, 1H) ppm; IR (NaCl):
3440, 2954, 2923, 2854, 1467, 1367, 1277, 1190, 1145, 1117, 1053, 954, 616 cm-1.
Sodium 9-decyl-15-hydroxy-12-methyl-4,7,10,13-tetraoxa-1-hexadecanesulfonate (11b).