Fluorometric Determination of Secondary Amines Based on Their Reaction with Fluorescamine

Article abstract of DOI:10.1021/ac50063a023

A spectrophotofluorometric method has been developed for the determination of secondary amines by using fluorescamine.When compounds having a secondary amino group were reacted with fluorescamine at pH 12 and then heated at 70 deg C for 10 min with a primary amine (L-Leu-L-Ala), they gave a bluish green fluorescence.By measurement of the fluorescence, most secondary amines could be determined in 0.5 nmol quantities.Relative standard deviations of 4.4 and 5.2percent are observed for the analyses of 5 nmol of N-methylaniline and sarcosine, respectively.