Magnetic Resonance in Chemistry p. 408 - 412 (1992)
Update date:2022-08-05
Topics:
Casu, Mariano
Lai, Adolfo
Erriu, Gianni
Onnis, Salvatore
Zucca, Nazario
Multilayer membranes of 1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine were prepared in D2O and exposed to γ-radiation.Changes in the chemical structure due to irradiation were studied by (1)H, (13)C and (31)P NMR after lyophilization and dissolution in chloroform-methanol (1:2 v/v).One- and two-dimensional techniques were used. 1-Palmitoyl-sn-glycero-3-phosphorylcholine, 2-palmitoyl-sn-glycero-3-phosphorylcholine, glycero-3-phosphorylcholine and palmitic acid were identified as new components.Their development during irradiation was quantitatively determined.The results were discussed in terms of possible reaction steps responsible for the radiolytic decomposition. Key words: (1)H NMR, (13)C NMR, (31)P NMR, 1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine, bilayer, radiation damage
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