Chemical and enzymatic synthesis of glycoconjugates 2. High yielding regioselective synthesis of N-acetyllactosamine by use of recombinant thermophilic glycosidases library

Article abstract of DOI:10.1016/S0040-4039(97)10141-1

β-Galactosidase activities from the recombinant thermophilic CLONEZYME(TM) glycosidase library were screened at 70°C for catalysis of a transgalactosylation from o-nitrophenyl-β-galactopyranoside to N-acetylglucosamine. Three thermophilic glycosidases (Gly001-06, -07 and -09) were found to produce predominantly the β(1-4)-linked isomer, Gal β(1-4)GlcNAc with up to 61% yield and less than 10% of the hydrolysis side reaction product. Thus, commercial recombinant thermophilic enzyme libraries constitute a novel class of biocatalysts for preparative organic synthesis.