Bioorganic and Medicinal Chemistry Letters p. 2593 - 2596 (2004)
Update date:2022-08-04
Topics:
Endo, Masayuki
Mitsui, Tsuneo
Okuni, Taeko
Kimoto, Michiko
Hirao, Ichiro
Yokoyama, Shigeyuki
Site-specific incorporation of a hydrophobic nucleotide analog into RNA, by T7 transcription mediated by unnatural base pairs, was developed. The nucleotide analog, 5-phenylethynyl-3-(β-D-ribofuranosyl)pyridin-2-one 5-triphosphate (denoted by Ph-yTP), was chemically synthesized and then site-specifically incorporated by T7 RNA polymerase into RNA opposite the pairing partner, 2-amino-6-(2-thienyl)purine (denoted by s) in DNA templates. The introduction of Ph-y into a theophylline-binding RNA aptamer, in which a uridine in the internal loop was replaced by Ph-y, raised the thermal stability of the aptamer. Thus, this unnatural nucleotide analog would be useful for stabilizing RNA tertiary structures and complexes between RNA and other molecules.
View MoreNanjing Chemical Material Corp.(NCMC)
website:http://www.njchemm.com
Contact:0086-25-83172879
Address:B12 Technology and Innovation Building, Nanjing Tech University, No.5 New Model Road
Henan zhongda Biological Engineering Co., Ltd
Contact:86-28-18109029985
Address:shenzhou road,xuedian industrial estate,zhengzhou city,henan province CHN
Huangshi Shennong Chemical Technology Co., Ltd
Contact:+86-714-3072290
Address:Eastern industrial park , Tieshan district , Huangshi city ,Hubei province , China
Contact:+86-731-84427351
Address:154 JIANXIANG SOUTH ROAD
jiangsu hualin chemical co.,ltd.
Contact:86-25-87787402
Address:jaingsu,china
Doi:10.1007/s11167-005-0389-6
(2005)Doi:10.1016/j.tetlet.2004.03.112
(2004)Doi:10.1021/jo00394a022
(1979)Doi:10.1039/C9RA00210C
(2019)Doi:10.1021/ja00500a019
(1979)Doi:10.1016/j.tetlet.2004.03.100
(2004)