Chemistry of Natural Compounds p. 105 - 108 (1987)
Update date:2022-08-03
Topics:
Snetkova, E. V.
Akulov, G. P.
Gordeeva, L. S.
Kaminskii, Yu. L.
Information is given on the synthesis of tritium-labeled UDPG by the successive transformation of D-<1-3H>glucose or D-<6-3H>glucose by the action of the enzymes hexokinase (EC 2.7.1.1), phosphoglucomutase (EC 2.7.5.1), and UDPG-pyrophosphorylase (EC 2.7.7.9) under conditions ensuring the retention of the tritium label.Methods of obtaining tritium-labelled substrates and intermediate products of their enzymatic transformations in the synthesis of <3H>UDPG are discussed.
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