Journal of the American Chemical Society p. 10640 - 10641 (2006)
Update date:2022-07-29
Topics:
Ueno, Tasuku
Urano, Yasuteru
Kojima, Hirotatsu
Nagano, Tetsuo
Nitrative stress is implicated in various pathogenic processes, including neurodegenerative disorders, but there is no practical fluorescence probe which can monitor the generation of nitrative stress with high selectivity. To design a suitable fluorescence probe, we have first focused on the fluorescence quenching mechanism of the nitro group, which has been believed to be a unique quencher of fluorescent dyes. We found that nitro group-based fluorescence quenching could be explained in terms of an electron transfer process, from the excited fluorophore to the electron-deficient aromatic nitro moiety. By utilizing this result, we succeeded in developing novel fluorogenic probes, NiSPYs, which can selectively monitor the generation of nitrative stress based on aromatic nitration. NiSPYs showed strong fluorescence enhancement upon the reaction with nitrating agents, including peroxynitrite, but showed little or no fluorescence augmentation in the presence of other reactive oxygen species. NiSPYs should be potentially useful as tools to study the role of nitrative stress in various biological applications. Copyright
View MoreChengdu Gelipu Biotechnology Co., Ltd.
website:http://www.glp-china.com
Contact:86-28-82610909
Address:chegndu
Suzhou Lixin Pharmaceutical Co., Ltd.
Contact:86-512-88169812
Address:21 Tangxi Road, Suzhou New District, Suzhou 215151
Contact:+86-28-88523492
Address:714rooms of Time Square, Pujiang County
Jiangsu Dacheng Pharmaceutical and Chemical Co.,Ltd
Contact:+86-0517-87036900
Address:Chuzhou Chemical park, Huai'an, Jiangsu Province
Nanjing Samwon International Limited
Contact:+86-25-84873444
Address:1108, BLDG B, New Century Plaza, No 1, South Taiping Rd.,
Doi:10.1134/S1070428014070069
(2014)Doi:10.1021/ja063247q
(2006)Doi:10.1039/c5nj02430g
(2016)Doi:10.1016/S0040-4020(01)92886-8
(1970)Doi:10.1021/om060615q
(2006)Doi:10.1021/jo01033a064
(1964)