Proline-catalyzed one-step asymmetric synthesis of 5-hydroxy-(2E)-hexenal from acetaldehyde

Article abstract of DOI:10.1021/jo015881m

For the first time, the L-proline-catalyzed direct asymmetric self-aldolization of acetaldehyde is described affording (+)-(5S)-hydroxy-(2E)-hexenal 2 with ee's ranging from 57 to 90%. Further transformations of 2 into synthetically valuable building bloc

Full text of DOI:10.1021/jo015881m

J . Org. Chem. 2002, 67, 301-303
301
Sch em e 1. Ald ola se-Ca ta lyzed Self-Ald oliza tion of
Aceta ld eh yd e
P r olin e-Ca ta lyzed On e-Step Asym m etr ic
Syn th esis of 5-Hyd r oxy-(2E)-h exen a l fr om
Aceta ld eh yd e
Armando Co´rdova, Wolfgang Notz, and
Carlos F. Barbas III*
The Skaggs Institute for Chemical Biology,
The Scripps Research Institute,
10550 North Torrey Pines Road,
La J olla, California 92037
Sch em e 2. P r olin e-Ca ta lyzed Self-Ald ol Rea ction
of Aceta ld eh yd e
carlos@scripps.edu
Received J une 29, 2001
affording the corresponding cross aldol products under
very mild conditions and often with excellent enantio-
selectivities.5,6 However, no aldehydes have been em-
ployed as aldol donors, and we became interested in
whether proline is capable of catalyzing the self-aldol
reaction of acetaldehyde to furnish polyketides in a
manner similar to DERA.
Abstr a ct: For the first time, the L-proline-catalyzed direct
asymmetric self-aldolization of acetaldehyde is described
affording (+)-(5S)-hydroxy-(2E)-hexenal 2 with ee’s ranging
from 57 to 90%. Further transformations of 2 into syntheti-
cally valuable building blocks are presented. A mechanism
for the formation of 2 is proposed.
In an initial experiment, a 4:1 mixture of DMSO/
acetaldehyde (10 mL) was treated with L-proline (35 mg)
as catalyst for 14 h at 23 °C. We observed the formation
of two products, which, after isolation and characteriza-
tion, were determined to be (+)-(5S)-hydroxy-(2E)-hex-
enal 2 and 2,4-hexadienal 3 (Scheme 2).
Triketide 2 was formed in 13% yield (w/w) and 57%
ee, together with 5% of 3. The absolute configuration of
the newly formed stereogenic center of 2 was established
to be S by comparison with its known optical rotation.7a
The formation of 2 is particularly noteworthy since this
transformation can be achieved in a single step by proline
catalysis as compared to the multistep syntheses of (S)-2
reported earlier.7a-c
The aldol reaction constitutes an important transfor-
mation in several biosynthetic pathways, particularly
those involving carbohydrates and polyketides. Whereas
carbohydrates are typically synthesized via a direct aldol
reaction by an aldolase enzyme,1 polyketide scaffolds are
constructed by modular polyketide synthases (PKSs) via
a Claisen condensation of two acyl-CoA units and sub-
sequent reduction of the â-keto moiety to afford the
corresponding â-hydroxy acyl-CoA.2
In 1994, Wong and co-workers described the stereo-
selective synthesis of polyketide precursors in a single
step. In their scheme, 2-deoxyribose-5-phosphate aldolase
(DERA) catalyzed the double-aldol sequence using only
acetaldehyde to afford cyclized trimer 1 (Scheme 1).3
As a complement to natural aldolases, we have devel-
oped catalytic antibodies such as 38C2 and 84G3 that
have a broad scope for aldol as well as mechanistically
related reactions providing products with excellent regio-,
diastereo-, and enantioselectivities.4
Encouraged by this result, we investigated a variety
of solvents and reaction temperatures and found pro-
nounced effects on both the yield and ee of 2 (Table 1).
(4) (a) Wagner, J .; Lerner, R. A.; Barbas, C. F., III. Science 1995,
270, 1797. (b) Bjo¨rnestedt, R.; Zhong, G.; Lerner, R. A.; Barbas, C. F.,
III. J . Am. Chem. Soc. 1996, 118, 11720. (c) Zhong, G.; Hoffmann, T.;
Lerner, R. A.; Danishefsky, S.; Barbas, C. F., III. J . Am. Chem. Soc.
1997, 119, 8131. (d) Barbas, C. F., III.; Heine, A.; Zhong, G.; Hoffmann,
T.; Gramatikova, S.; Bjo¨rnestedt, R.; List, B.; Anderson, J .; Stura, E.
A.; Wilson, I. A.; Lerner, R. A. Science 1997, 278, 2085. (e) Hoffmann,
T.; Zhong, G.; List, B.; Shabat, D.; Anderson, J .; Gramatikova, S.;
Lerner, R. A.; Barbas, C. F., III. J . Am. Chem. Soc. 1998, 120, 2768.
(f) Zhong, G.; Shabat, D.; List, B.; Anderson, J .; Sinha, S. C.; Lerner,
R. A.; Barbas, C. F., III. Angew. Chem., Int. Ed. 1998, 37, 2481. (g)
Sinha, S. C.; Barbas, C. F., III.; Lerner, R. A. Proc. Natl. Acad. Sci.
U.S.A. 1998, 95, 14603. (h) List, B.; Lerner, R. A.; Barbas, C. F., III.
Org. Lett. 1999, 1, 59. (i) List, B.; Shabat, D.; Zhong, G.; Turner, J .
M.; Li, A.; Bui, T.; Anderson, J .; Lerner, R. A.; Barbas, C. F., III. J .
Am. Chem. Soc. 1999, 121, 7283. (j) Zhong, G.; Lerner, R. A.; Barbas,
C. F., III. Angew. Chem., Int. Ed. 1999, 38, 3738. (k) Tanaka, F.;
Lerner, R. A.; Barbas, C. F., III. J . Am. Chem. Soc. 2000, 4835.
(5) (a) List, B.; Lerner, R. A.; Barbas, C. F., III. J . Am. Chem. Soc.
2000, 122, 2395. (b) Notz, W.; List, B. J . Am. Chem. Soc. 2000, 122,
7386. (c) Bui, T.; Barbas, C. F., III. Tetrahedron Lett. 2000, 41, 6951.
(d) Sakthivel, K.; Notz, W.; Bui, T.; Barbas, C. F., III. J . Am. Chem.
Soc. 2001, 123, 5260. (e) Hajos, Z. G.; Parrish, D. R. J . Org. Chem.
1974, 39, 1615. (f) Eder, U.; Sauer, G.; Wiechert, R. Angew. Chem.,
Int. Ed. Engl. 1971, 10, 496. (g) Agami, C.; Platzer, N.; Sevestre, H.
Bull. Soc. Chim. Fr. 1987, 2, 358.
Yet, to date, when aldehydes were used as donors in
cross- as well as self-aldolizations, these antibodies have
only afforded the corresponding aldol condensation
products.4e Expanding our efforts in this field, we recently
reported the proline-catalyzed direct asymmetric aldol
reaction between simple ketones and various aldehydes
* To whom correspondence should be addressed. Fax: (+1) (858)
784-2583.
(1) For excellent reviews on the use of natural aldolase enzymes,
see: (a) Gijsen, H. J . M.; Qiao, L.; Fitz, W.; Wong, C.-H. Chem. Rev.
1996, 96, 443. (b) Wong, C.-H.; Halcomb, R. L.; Ichikawa, Y.; Kajimoto,
T. Angew. Chem., Int. Ed. Engl. 1995, 34, 412. (c) Wong, C.-H.;
Whitesides, G. M. Enzymes in Synthetic Organic Chemistry; Pergamon
Press: Oxford, 1994. (d) Bednarski, M. D. In Comprehensive Organic
Synthesis; Trost, B. M., Ed.; Pergamon Press: Oxford, 1991; Vol. 2, p
455. (e) Machajewski, T. D.; Wong, C.-H. Angew. Chem., Int. Ed. 2000,
39, 1352. (f) Koeller, K. M.; Wong, C.-H. Nature 2001, 409, 232. (g)
Wymer, N.; Buchanan, L. V.; Hernderson, D.; Mehta, N.; Botting, C.
H.; Pocivavsek, L.; Fierke, C. A.; Toone, E. J .; Naismith, J . H. Structure
2001, 9, 1. (h) Wymer, N.; Toone, E. J . Curr. Opin. Chem. Biol. 2000,
4, 110.
(6) L-Proline and its derivatives also catalyzed asymmetric Mannich-
and Michael-type reactions. See: (a) Betancort, J . M.; Barbas, C. F.,
III. Org. Lett. 2001, 3, 3737. (b) Betancort, J . M.; Sakthivel, K.;
Thayumanavan, R.; Barbas, C. F., III. Tetrahedron Lett. 2001, 42, 4441.
(c) Notz, W.; Sakthivel, K.; Bui, T.; Barbas, C. F., III. Tetrahedron Lett.
2001, 42, 199. (d) List, B. J . Am. Chem. Soc. 2000, 122, 9337.
(2) (a) Khosla, C. J . Org. Chem. 2000, 65, 8127 and references cited
therein. (b) Kinoshita, K.; Williard, P. G.; Khosla, C.; Cane, D. E. J .
Am. Chem. Soc. 2001, 123, 2495. (c) Khosla, C.; Harbury, P. B. Nature
2001, 409, 247.
(3) Gijsen, H. J . M.; Wong, C.-H. J . Am. Chem. Soc. 1994, 116, 8422.
10.1021/jo015881m CCC: $22.00 © 2002 American Chemical Society
Published on Web 12/13/2001

302 J . Org. Chem., Vol. 67, No. 1, 2002
Notes
Ta ble 1. P r olin e-Ca ta lyzed Self-Ald ol Rea ction of
Aceta ld eh yd e u n d er Differ en t Rea ction Con d ition s
Sch em e 3. P r op osed Rea ction Mech a n ism for th e
P r olin e-Ca ta lyzed Self-Ald ol Rea ction of
Aceta ld eh yd e
solvent
T (°C)
time (h)
eea (%)
yield of 2b
acetonitrile
acetonitrile
DMSO
dioxane
dioxane
EtOAc
THF
23
4
23
23
0
23
23
4
14
5
14
14
5
14
14
14
5
66
69
57
69
82
57
69
84
9
5
13
7
9
3
8
12
THF
THF
0
90
10
Sch em e 4. Tr a n sfor m a tion of 2 in to Syn th etica lly
NMP
neat
chloroform
toluene
MTBE
octane
23
23
23
23
23
23
14
14
14
14
14
14
56
57
68
n.d.
n.d.
n.d.
4
5
2
Va lu a ble Syn th on s 4-7a
traces
traces
traces
a
Determined by chiral stationary phase HPLC. See the Ex-
b
perimental Section. Isolated yields in w/w % after column chro-
matography. In
a typical experiment, a mixture of solvent/
acetaldehyde (4:1, 10 mL) and L-proline (35 mg) was stirred at
the indicated temperature for the indicated period of time. The
crude reaction mixture was filtered through silica gel and then
purified by column chromatography.
a
Reagents and conditions: (a) NaClO2, KH2PO4, 2-methyl-2-
butene, t-BuOH/H2O, 89%; (b) CH2N2, Et2O, 96%; (c) NaBH4,
MeOH/THF, 92%.
Whereas 2 was readily formed at 23 °C within 14 h in
polar aprotic solvents with ee’s ranging from 56 to 69%,
only trace amounts of 2 were produced in nonpolar
solvents such as toluene and octane where the major
product formed was diene 3. Decreasing the reaction
temperature to 0-4 °C not only resulted in an increase
of the yield and ee of 2 but also diminished the formation
of side product 3.8 The best results were obtained using
anhydrous THF at 0 °C, thereby affording 2 with an ee
of 90%. We also performed the reaction on a larger scale
(20% acetaldehyde/THF, 500 mL) at 4 °C with D-proline
(1.2 g) yielding (R)-2 (2.9 g) with 84% ee together with
side product 3 (0.5 g).
The stereochemical result of this self-aldol reaction is
in accordance with our previously proposed transition-
state model for the proline-catalyzed aldol reaction of
acetone with aldehydes.5d In the case of acetaldehyde, an
analogous enamine is involved in a re-facial attack of the
carbonyl group of acetaldehyde (Scheme 3). After the
carbon-carbon bond-forming step, however, we assume
that the resulting reactive iminium ion, instead of being
hydrolyzed, might react further in a Mannich-type con-
densation9 to afford 2.
aldehyde. This is consistent with our earlier observation
that no cross-aldolization occurred between acetone and
hexenal or cinnamaldehyde.10 Interestingly, the forma-
tion of hemiacetal 1 was not catalyzed by proline.
Aldehyde 2 is a versatile synthon for other syntheti-
cally valuable building blocks. For example, the aldehyde
functionality of 2 can be readily oxidized (NaClO2) or
reduced (NaBH4) to afford the corresponding carboxylic
acid 411 or allylic alcohol 5,7c respectively (Scheme 4).
Furthermore, carboxylic acid 4 can be readily trans-
formed into aldehyde 7 upon treatment of methyl ester
6 with benzaldehyde and catalytic amounts of KHMDS
and subsequent reduction with DIBALH.12 Thus, the
stereogenic center at C-3 that would have been originally
obtained from a double aldol addition reaction can be
restored with complete stereoselectivity via internal
Michael addition of the hemiacetal. Attempts to achieve
the direct conversion of 2 to 7, however, were unsuccess-
ful.13
Compounds 2, 4, 5, and 7 and their enantiomers are
also structural motifs common to important macrolide
antibiotics such as Grahamimycin A and A1, Carbomycin
B and Platenomycin.7c,11,12a For implementation of these
building blocks in total synthesis, products (R)-2 and
(S)-2 could be readily obtained on gram scale in enan-
tiomerically pure form via kinetic resolution by Candida
antarctica lipase B (CALB) using vinyl acetate as acetyl
donor followed by separation of the corresponding acetate
(R)-811 and unreacted (S)-2. Subsequent enzymatic hy-
drolysis of (R)-8 yielded (R)-2 in > 99% ee and 95% yield
(Scheme 5).
In contrast to the DERA-catalyzed reaction, where the
reaction is terminated after two aldol additions by
formation of hemiacetal 1, the proline-catalyzed reaction
does not proceed beyond the formation of 2 since this
product is no longer reactive enough to undergo an
additional aldol addition with another molecule of acet-
(7) Several syntheses of 2 and its enantiomer have been described.
See: (a) Lichtenthaler, F. W.; Klingler, F. D.; J arglis, P. Carbohydr.
Res. 1984, 132, C1. (b) Nakajima, N.; Uoto, K.; Yonemitsu, O.; Hata,
T. Chem. Pharm. Bull. 1991, 39, 64. (c) Keck, G. E.; Palani, A.;
McHardy, S. F. J . Org. Chem. 1994, 59, 3113. For the synthesis of
(S)-ethyl (â)-hydroxybutyrate, see: (d) Seebach, D.; Zu¨ger, M. Helv.
Chim. Acta 1982, 65, 495. (e) Noyori, R.; Ohkama, T.; Kitamura, M.;
Takaya, H.; Sayo, N.; Kumobayashi, H.; Akutagawa, S. J . Am. Chem.
Soc. 1987, 109, 5856.
(10) Unpublished results.
(11) Hillis, L. R.; Ronald, R. C. J . Org. Chem. 1985, 50, 470.
(12) (a) Hayes, C.; Heathcock, C. H. J . Org. Chem. 1997, 62, 2678.
(b) Evans, D. A.; Gauche-Prunet, J . A. J . Org. Chem. 1993, 58, 2446.
(13) Application of this protocol to the corresponding unsaturated
hydroxy ketones by Evans et al. were also unsuccessful. See ref 12b.
(8) Performing the reaction at temperatures below 0 °C did not
provide further improvements in the ee of 2.
(9) Ishikawa, T.; Uedo, E.; Okada, S.; Saito, S. Synlett 1999, 450.

Notes
J . Org. Chem., Vol. 67, No. 1, 2002 303
(5S)-Hyd r oxy-(2E)-h exen a l ((S)-2). A mixture of THF/
acetaldehyde (4:1, 500 mL) and L-proline (1.2 g) was stirred for
14 h at 4 °C. The crude reaction mixture was filtered through
silical gel and then purified by flash column chromatography
(hexanes/ethyl acetate ) 1:1) to afford (S)-2 (2.9 g) together with
3 (0.5 g). The ee was determined by chiral stationary-phase
HPLC analysis using a Chiralpak Daicel AD-RH column and
eluting with 15% acetonitrile/water (0.1% TFA), flow rate 0.7
mL/min, λ ) 272 nm. (S)-enantiomer of 2: tR ) 15.74 min. (R)-
enantiomer of 2: tR ) 19.96 min.
1,(5S)-Dih yd r oxy-(2E)-h exen e (5). To a solution of (S)-2
(114 mg, 1 mmol) in MeOH/THF (10 mL, 1:1) was added sodium
borohydride, and the mixture was stirred for 15 min. The solvent
was removed, and after addition of water and extraction with
diethyl ether, the organic phase was dried (MgSO4), concentrated
in vacuo, and purified by flash column chromatography (hexanes/
ethyl acetate ) 1:4) to afford 57c (106 mg, 92%).
Meth yl (5S)-Hyd r oxy-(2E)-h exen oa te (6). To a magneti-
cally stirred solution of (S)-2 (100 mg, 0.87 mmol) in tert-butyl
alcohol/water (5:1, 10 mL) were added successively NaH2PO4
(180 mg, 1.5 mmol), 2-methyl-2-butene (3 mL, 2 M solution in
THF, 6.0 mmol), and NaClO2 (270 mg, 3.0 mmol). The resulting
mixture was stirred for 4 h until the yellow solution turned
colorless. The solvent was removed under reduced pressure, and
the residue was extracted with ethyl acetate, washed with water
and brine, and dried over MgSO4. The combined organic layers
were concentrated, and the residual acid 411 was dissolved in
diethyl ether (10 mL) and treated with excess diazomethane in
diethyl ether, the excess being consumed by addition of acetic
acid. Concentration in vacuo, coevaporation with toluene, and
purification of the residue by flash chromatography (hexanes/
ethyl acetate ) 1:1) afforded methyl ester 612 (106 mg, 0.73
mmol, 85%).
Kin etic Resolu tion w ith CALB. A 0.87 M solution of (R)-2
(ee ) 82%) in anhydrous dichloromethane was treated with 1
equiv of vinyl acetate and 100 mg of CALB for 16 h at room
temperature. After filtration of the enzyme, the reaction mixture
was concentrated and the residue purified by flash column
chromatography (hexanes/ethyl acetate ) 1:1) affording quan-
titatively enantiomerically pure (S)-2 and (R)-8.11 (R)-8 was
subsequently deacetylated in a 1:1-mixture of dioxane/PBS
buffer (20 mL) using CALB (100 mg) to provide enantiomerically
pure (R)-2.
Sch em e 5. P r ep a r a tion of En a n tiom er ica lly P u r e
2 by Kin etic Resolu tion w ith Ca n d id a a n ta r ctica
Lip a se Ba
a
Reagents and conditions: (a) Candida antarctica lipase B
(CALB), vinyl acetate, CH2Cl2; (b) CALB, dioxane/PBS buffer (1:
1), 95%.
In summary, for the first time, we have demonstrated
the proline-catalyzed direct asymmetric self-aldolization
of acetaldehyde affording triketide 2 on a multigram scale
with high ee. This reaction is operationally facile, and
compared to other syntheses, cost and time efficient.
Triketide 2 can be readily transformed into a number of
other chiral building blocks. Further investigations of this
reaction with respect to its mechanism as well as the
implementation of 2 in total synthesis are in progress
and will be reported in due course.
Exp er im en ta l Section
Gen er a l Meth od s. Chemicals and solvents were either
purchased puriss p.A. from commercial suppliers or purified by
standard techniques. For thin-layer chromatography (TLC),
silica gel plates Merck 60 F254 were used and compounds were
visualized by irradiation with UV light and/or by treatment with
a solution of phosphomolybdic acid (25 g), Ce(SO4)2‚H2O (10 g),
concentrated H2SO4 (60 mL), and H2O (940 mL) followed by
heating or by treatment with a solution of p-anisaldehyde (23
mL), concentrated H2SO4 (35 mL), acetic acid (10 mL), and
ethanol (900 mL) followed by heating. Flash chromatography
was performed using silica gel Merck 60 (particle size 0.040-
0.063 mm). HPLC was carried out using a Hitachi organizer
consisting of a D-2500 Chromato-Integrator, a L-4000 UV-
detector, and a L-6200A intelligent pump. Optical rotations were
recorded on a Perkin-Elmer 241 polarimeter (λ ) 589 nm, 1 dm
cell). The lipase (component B) Novozym 435 derived from C.
antarctica is a product of Novo Nordisk A/S Denmark. The
enzyme used was an immobilized preparation on a macroporous
poly(acrylic) resin, containing 1% (w/w) enzyme, with a catalytic
activity of approximately 25 000 LU/g preparation. CALB was
dried in a desiccator over P2O5 prior to use.
Ack n ow led gm en t. This study was supported in
part by the NIH (CA27489) and The Skaggs Institute
for Chemical Biology. The provision of enzyme by Novo
Nordisk A/S, Denmark, is also gratefully acknowledged.
J O015881M