SYNTHESIS WITH AN IMMOBILIZED ENZYME OF N-ACETYL-9-O-ACETYL-NEURAMINIC ACID, A SUGAR REPORTED AS A COMPONENT OF EMBRYONIC AND TUMOR ANTIGENS.

Article abstract of DOI:10.1016/S0040-4039(00)94847-0

Condensation of 2-acetamido-6-O-acetyl-2-deoxy-D-mannose with pyruvic acid in the presence of immobilized acylneuraminate pyruvate lyase (2.8 units) gave 5-acetamido-9-O-acetyl-3,5-dideoxy-D-glycero-D-galacto-2-nonulopyranos-1-onic acid (N-acetyl-9-O-acetylneuraminic acid) on the 0.3 mmole scale.