Reaction Gas Chromatography without Solvent for Identification of Nanogram Quantities of Natural Products

Article abstract of DOI:10.1021/ac00259a043

A method is described for trapping nanogram quantities of compounds or mixtures from a gas chromatography column with approximately 85percent efficiency and reinjecting the sample on the same or different column, with or without carrying out a reaction, and all without contamination with solvent.The method includes a solvent-free ozonolysis technique for determining the position of double bonds in alkenes by using 200 ng of sample, even when low molecular weight carbonyl compounds are produced.Characterization of carbonyl compounds by sodium borohydride reduction, hydrogenation of alkenes, and cleavage of epoxides by periodic acid at this scale, without the use of solvents, are also described.