
Bulletin of the Chemical Society of Japan p. 2349 - 2351 (1992)
Update date:2022-08-16
Topics:
Yatome, Chizuko
Fujimi, Kazuhiko
Ogawa, Toshihiko
The effect of inhibitors on the azo-reduction of 4'-dimethylaminoazobenzene-2-carboxylic acid (DMABC) by Clostridium diaphorase together with β-nicotinamide adenine dinucleotide, reduced form (NADH) has been investigated.The reduction was inhibited by o-aminobenzoic acid which is a reduction product of DMABC.Lineweaver-Burk plots indicated that 0-aminobenzoic acid was a competitive inhibitor with respect to DMABC (Ki=10.5*10-3 mol dm-3).Various monosubstituted benzoic acid derivatives were also competitive inhibitors, among which 0-hydroxybenzoic acid was most effective.
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