Syntheses of enantiopure aliphatic secondary alcohols and acetates by bioresolution with lipase B from candida antarctica

Article abstract of DOI:10.3390/molecules17088955

The lipase B from Candida antarctica (Novozym 435, CALB) efficiently catalyzed the kinetic resolution of some aliphatic secondary alcohols: (±)-4-methylpentan- 2-ol (1), (±)-5-methylhexan-2-ol (3), (±)-octan-2-ol (4), (±)-heptan-3-ol (5) and (±)-oct-1- en-3-ol (6). The lipase showed excellent enantioselectivities in the transesterifications of racemic aliphatic secondary alcohols producing the enantiopure alcohols (>99% ee) and acetates (>99% ee) with good yields. Kinetic resolution of rac-alcohols was successfully achieved with CALB lipase using simple conditions, vinyl acetate as acylating agent, and hexane as non-polar solvent.

Full text of DOI:10.3390/molecules17088955

Molecules 2012, 17, 8955-8967; doi:10.3390/molecules17088955
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molecules
ISSN 1420-3049
www.mdpi.com/journal/molecules
Article
Syntheses of Enantiopure Aliphatic Secondary Alcohols and
Acetates by Bioresolution with Lipase B from Candida antarctica
Hercules V. Ferreira 1, Lenilson C. Rocha 1, Richele P. Severino 1,2 and André L. M. Porto 1,*
1
Institute of Chemistry of São Carlos, University of São Paulo, Av. Trabalhador São-carlense, 400,
13560-970, São Carlos, SP, Brazil; E-Mails: hercules_quimica@hotmail.com (H.V.F.);
lenilson@iqsc.usp.br (L.C.R.); richeleps@yahoo.com.br (R.P.S.)
2
Department of Chemistry, Federal University of Goias, Campus Advanced of Catalão,
Av. Dr. Lamartine Pinto de Avelar, 1120, 75704-020, Catalão, GO, Brazil
* Author to whom correspondence should be addressed; E-Mail: almporto@iqsc.usp.br;
Tel.: +55-16-3373-8103; Fax: +55-16-3372-9952.
Received: 24 April 2012; in revised form: 27 June 2012 / Accepted: 3 July 2012 /
Published: 26 July 2012
Abstract: The lipase B from Candida antarctica (Novozym 435®, CALB) efficiently
catalyzed the kinetic resolution of some aliphatic secondary alcohols: (±)-4-methylpentan-
2-ol (1), (±)-5-methylhexan-2-ol (3), (±)-octan-2-ol (4), (±)-heptan-3-ol (5) and (±)-oct-1-
en-3-ol (6). The lipase showed excellent enantioselectivities in the transesterifications of
racemic aliphatic secondary alcohols producing the enantiopure alcohols (>99% ee) and
acetates (>99% ee) with good yields. Kinetic resolution of rac-alcohols was successfully
achieved with CALB lipase using simple conditions, vinyl acetate as acylating agent, and
hexane as non-polar solvent.
Keywords: kinetic resolution; lipase; Candida antarctica; aliphatic secondary alcohols
1. Introduction
The use of enzymes for organic synthesis has become an interesting area for organic and bio-organic
chemists. Since many enzymes have been demonstrated to possess activity against non-natural
substrates in organic media they have become widely use to carry out synthetic transformations.
Hydrolases are the most frequently used enzymes due to their broad substrate spectrum and
considerable stability. Additionally, many of them are commercially available and they work under

Molecules 2012, 17
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mild reaction conditions and without the necessity for cofactors [1]. Enantiomerically pure alcohols
and esters are key intermediates for asymmetric synthesis of pharmaceutical and agrochemical
compounds [2,3]. Among the numerous synthetic methods for asymmetric synthesis, enzyme-catalysed
kinetic resolution of racemic alcohols through transesterification is an attractive route. For example,
the enantiomeric resolution of atenolol by lipase B from Candida antarctica [4]. Transesterification
reactions catalysed by hydrolytic enzymes have been extensively studied in conventional nonaqueous
solvents, producing excellent results [5–9]. In addition, the dynamic kinetic resolution (DKR) is a
useful methodology for the conversion of racemic substrates to single enantiomer [10,11].
Lipases are frequently used as an asymmetric catalyst for the preparation of enantiomerically pure
organic compounds [12,13]. Lipase B from C. antarctica (Novozym 435®) displayed esterification of
aliphatic secondary rac-alcohols producing good enantiomeric purities to acetates (88.7–97.7% ee) and
alcohols (42.8–85.5% ee) [14]. The lipase from Pseudomonas fluorescens (Amano AK) catalysed the
transesterification reactions of secondary alcohols (2-butanol, 2-pentanol, 2-hexanol, 2-heptanol and
2-octanol) using vinyl acetate as acylating agent and producing moderate selectivities for the
products [15].
The resolution of a racemic substrate can be achieved with a range of hydrolases including lipases
and esterases. Kinetic resolution (KR) of racemic secondary alcohols with lipases has been shown
the dependence between the structure of the substrate and the enantioselectivity of enzymatic
transesterification in according to the empirical Kazlauskas’ rule [16].
Recently we reported the use of enzyme-catalysed transesterifications of secondary alcohols by
kinetic resolution using different compounds to produce enantiomerically pure or enriched alcohols
and acetates [17–20]. In this work, we report an efficient protocol for the KR of aliphatic secondary
alcohols using mild conditions to produce enantiopure compounds by lipase CALB.
2. Results and Discussion
Rocha et al. reported the kinetic resolution of secondary iodophenylethanols using lipase B from
Candida antarctica (CALB) to produce alcohols and acetates with high enantiomeric excesses [17].
Efficient KR of rac-alcohols is successfully achieved by lipase under mild friendly conditions, vinyl
acetate as acylating agent, and hexane as non-polar solvent. To extend this biocatalytic methodology, a
series of aliphatic secondary alcohols 1–6 were used in order to obtain enantiopure products. Initially,
the racemic alcohols 1–6 and acetates 7–12 were obtained with good yields by conventional methods
(Scheme 1).
In general, the KR of aromatic secondary alcohols produced enantiopure compounds with CALB.
However, the enzymatic kinetic resolution of aliphatic secondary alcohols did not show an effective
enantioresolution, and low enantiomeric excesses are frequently obtained. In this study, our focus on
the KR enabled a series of aliphatic secondary alcohols containing different groups attached to
the stereogenic center. The results are summarized in Table 1. The kind of group attached to the
stereogenic center is crucial for the useful kinetic resolution of secondary alcohols by CALB. The
racemic substrates 1, 3–4 were efficiently resolved by CALB producing the enantiopure (S)-alcohols
(1, 3–4) and (R)-acetates 7, 9–10 with high enantiomeric excesses (>99% ee, Table 1). The studies, of

Molecules 2012, 17
8957
the rac-alcohols 1, 3–4 contain the methyl group attached to the stereogenic center, which were
responsible for the high selectivities obtained by CALB.
Scheme 1. Chemoenzymatic resolution of aliphatic secondary alcohols by C. Antarctica lipase B.
OAc
R2
OH
OH
R2
CALB, hexane
+
)
R1
R1
R2
R1
vinyl acetate
150 rpm, 32oC
rac-alcohols
1 3-5
(S)-alcohols ( ,
7 9-11
(R)-acetates ( ,
(S)-acetate (
>99 % ee
)
1-6
(
)
6
(R)-alcohol ( )
12
)
>99 % ee
R1 = i-Bu, R2 = Me (1)
2
R1 = n-Pr, R2 = i-Pr ( )
R1 = i-Pent, R2 = Me (3)
R1 = n-Hex, R2 = Me (4)
5
R1 = n-Bu, R2 = Et ( )
R1 = n-Pent, R2 = vinyl (6)
Table 1. Enzymatic transesterification of (±)-secondary alcohols 1–6 by lipase B from
Candida antarctica a.
alcohols 1–6
acetates 7–12
alcohols
t (min) c(%)b* ee (%) b ac b yield (%) b,d c (%) c ee (%) c ac c yield (%) c,d
E
1
2
3
60
60
50
100 e
60
>99
S
-
40
-
50
-
>99
-
R
-
45
-
>200
-
-
20
-
-
-
40
50
30
40
50
30
50
20
45
50
>99
>99
>99
>99
>99
-
-
-
-
40
50
>99 f
S
-
32
-
R
-
31
-
>200
4
5
20
70
-
-
40
60
-
-
-
-
-
-
60
50
>99 f
S
-
40
-
R
-
35
-
>200
240
420
20
70
-
>99
-
-
>200
-
50
S
-
35
-
>99
-
R
-
32
-
80
6
40
55
-
-
-
-
-
-
-
60
50
>99 f
R
41
>99
S
50
>200
a
The reactions were carried out in a Erlenmeyer flask containing 10 mL of hexane (HPLC grade),
0.5 mL of vinyl acetate, 0.5 mmol of racemic alcohols 1–6 (0.5 mmol) and 80 mg of lipase CALB.
The reaction mixture was stirred in an orbital shaker (32 C, 150 rpm) until 50% of conversion;
b* concentrations of unreacted alcohols; c acetates; d isolated yield; t: time (minutes); c: conversion.
e
Not reacted; ee: enantiomeric excesses determined by chiral GC; ac: absolute configuration;
f
E: enantiomeric ratio was calculated using Sih’s method [14]; Enantiomeric excesses obtained
after acetylation of alcohols.
In addition, we investigated the selectivity of lipase by increasing the steric bulk attached to the
stereogenic center in other racemic alcohols. For the aliphatic alcohol 2, with n-propyl and isopropyl
groups linked to the stereogenic center, the enzymatic KR for the production of acetate 8 not occurred.
These results confirm that the n-propyl and isopropyl are large groups for the KR of secondary
alcohols by lipase B from C. antarctica.

Molecules 2012, 17
8958
However, the KR for alcohols 5–6 were highly efficient, producing enantiopure products (>99% ee,
Table 1). In these cases, the substituent groups attached to the stereogenic center were n-butyl and
n-ethyl for 5 and n-pentyl and vinyl for 6. Moreover, for the aliphatic alcohol 5 the reaction occurred
slowly and the time necessary to reach 50% conversion was 7 hours (Table 1). In this investigation, the
alkyl and vinyl groups were accepted by CALB, giving alcohols and acetates with excellent optical
purities. In general, high selectivity for these reactions was obtained (E > 200). Recently, the
chemoenzymatic resolution of racemic allylic alcohol 6 was described using CALB [21,22]. Lipase
from Pseudomonas cepacia catalysed esterification of allylic alcohols with different selectivities, but
in several cases not exceeding 98% ee to the compounds [23].
According to the empirical Kazlauskas rule, lipase stereoselectivity is mainly set by steric
interactions between enzyme and substrate. The small difference in the steric bulk of ethyl and vinyl
groups on aliphatic alcohols 5–6 compared with the methyl group on alcohols 1, 3–4, showed the
occurrence of non-steric interactions, and these rac-alcohols reacted by lipase CALB.
The assignment of the absolute configuration for (S)-alcohols 1, 4–5, (R)-alcohol 6, (R)-acetate 7
and (S)-acetate 12 were done on the basis of their specific rotation values and compared with literature
values. In addition, these data were confirmed with the empirical Kazlauskas rule. This rule shows the
enantiopreference of the esterification of secondary alcohols by lipase and suggests the attribution of
the absolute configuration of products. The absolute configurations of (R)-acetates 10–11 were
suggested by the empirical Kazlauskas rule [5,24].
The enantiomeric excesses of alcohol 1 and acetates (7, 9–11) were determined by chiral column
chromatography. The rac-alcohols 3–6 did not show enantioseparation on the chiral column
chromatography used. In these cases, these alcohols were derivatized with pyridine/anhydride acetic to
produce the corresponding acetates 9–12. The enantiomeric excesses of its acetate derivatives were
determined by gas chromatography with an FID detector using chiral column (Table 1).
3. Experimental
3.1. General
Vinyl acetate, aliphatic ketones (4-methylpentan-2-one, 2-methylhexan-3-one, 5-methylhexan-2-
one, 5-methylhexan-2-one, 5-methylhexan-2-one, oct-1-en-3-one), Ac2O, pyridine, NaBH4 were
purchased from Sigma-Aldrich (St. Louis, MO, USA), and the solvents (AcOEt, hexane) were
purchased from Synth (São Paulo, SP, Brazil). Tecnal TE-421 (São Paulo, SP, Brazil) or Superohm
G-25 (Piracicaba, SP, Brazil) orbital shakers were employed in the KR reactions. The purification of
the products was carried out by column chromatography (CC) over silica gel (230–400 mesh)
eluted with mixtures of hexane and AcOEt. The collected fractions were monitored by TLC on
aluminum-backed pre-coated silica gel 60 F254 (Sorbent-Technologies) layers eluted with hexane and
AcOEt, and visualized by spraying with p-anisaldehyde/H2SO4 reagent followed by heating at ca. 60.
Syntheses of (±)-aliphatic secondary alcohols 1–6 and acetates 7–12 are describes in Scheme 2. NMR
spectra were recorded on a Bruker AC-200 spectrometer using CDCl3 as solvent and TMS as
internal calibration.

Molecules 2012, 17
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Scheme 2. Preparations of (±)-alcohols 1–6 and (±)-acetates 7–12.
OAc
R2
rac-acetates (7-12)
O
OH
R2
NaBH4
Ac2O
R1
R1
R2
R1
pyridine
methanol
rac-alcohols (1-6)
R1 = i-Bu, R2 = Me (1)
2
R1 = n-Pr, R2 = i-Pr ( )
R1 = i-Pent, R2 = Me (3)
R1 = n-Hex, R2 = Me (4)
5
R1 = n-Bu, R2 = Et ( )
R1 = n-Pent, R2 = vinyl (6)
3.1.1. Preparation of (±) Aliphatic Secondary Alcohols
Racemic secondary alcohols 1–6 were prepared by reducing commercial ketones (4-methylpentan-
2-one, 2-methylhexan-3-one, 5-methylhexan-2-one, 5-methylhexan-2-one, 5-methylhexan-2-one,
oct-1-en-3-one) using sodium borohydride in methanol. The ketones (5 mmol), NaBH4 (5.5 mmol) and
methanol (10 mL) were added to a 25 mL flask equipped with a magnetic stirrer. The mixtures were
stirred for 4 h at r.t. (Scheme 1). Next, the reactions were quenched by the addition of water (1 mL),
the methanol was removed under vacuum and the residue was extracted with ethyl acetate (3 × 20 mL).
The combined organic phases were dried over MgSO4 and then filtered. The organic solvent was
evaporated under reduced pressure and the residue was purified by silica gel column chromatography
using hexane and ethyl acetate as eluent to produce racemic alcohols 1–6 in excellent yields (1, 70%;
2, 95%; 3, 92%; 4, 97%; 5, 94%; 6, 93%). The spectroscopic data (1H-NMR, MS and IR) of alcohols
1, 2, 4 and 6 agreed with the data reported in the literature [22,25–28].
1
5-Methylhexan-2-ol (3): H-NMR (300 MHz, CDCl3), δ (ppm): 3.74 (1H, m), 1.72 (1H, br, OH),
1.05–1.58 (5H, m), 1.16 (3H, d, J = 6.0 Hz), 0.87 (6H, d, J = 6.0 Hz). 13C-NMR (75 MHz, CDCl3), δ
(ppm): 68.4 (CH), 37.1 (CH2), 34.9 (CH2), 28.0 (CH), 23.4 (CH3), 22.6 (CH3), 22.5 (CH3).
1
Heptan-3-ol (5): H-NMR (300 MHz, CDCl3), δ (ppm): 3.47 (1H, m), 1.61 (1H, br, OH), 1.55–1.15
(8H, m), 0.92–0.83 (6H, br t, CH3-CH2). 13C-NMR (75 MHz, CDCl3), δ (ppm): 73.2 (CH), 36.6 (CH2),
30.1 (CH2), 27.8 (CH2), 22.7 (CH2), 14.0 (CH3), 9.8 (CH3).
3.1.2. Preparation of (±) Aliphatic Secondary Acetates
Alcohols 1–6 (3.0 mmol), pyridine (0.5 mL, 6.2 mmol) and Ac2O (0.5 mL, 5.3 mmol) were added
to a 25 mL flask equipped with a magnetic stirrer. The mixture was stirred for 24 h at r.t. (Scheme 2).
The reactions were quenched by the addition of 10% HCl (2 mL), and the organic phase was extracted
with ethyl acetate (3 × 20 mL). The combined organic phases were dried over MgSO4 and then filtered.
The organic solvent was evaporated under reduced pressure and the residue was purified by silica gel
column chromatography using hexane and ethyl acetate as eluent to give racemic acetates 7–12 in
good to high yields (7, 80%; 8, 87%; 9, 85%; 10, 92%; 11, 89%; 12, 93%). The spectroscopic data
(1H-NMR, MS and IR) of acetates 7, 10 and 12 agreed with the data reported in the literature [22,25,27].

Molecules 2012, 17
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1
5-Methylhexan-2-yl acetate (9): H-NMR (300 MHz, CDCl3), δ (ppm): 4.85 (1H, m), 2.00 (3H, s),
1.45–1.55 (3H, m), 1.05–1.20 (1H, overlap), 1.18 (3H, d, J = 6.0 Hz), 0.86 (6H, d, J = 6.0 Hz).
13C-NMR (75 MHz, CDCl3), δ (ppm): 170.8, 71.3, 34.4, 33.7, 27.9, 22.5 (2C), 21.3, 20.0.
Heptan-3-yl acetate (11): 1H-NMR (300 MHz, CDCl3), δ (ppm): 4.79 (1H, m), 2.02 (3H, s), 1.61–145
(4H, m), 1.35–1.20 (4H, m), 0.89-0.84 (6H, overlap, CH3-CH2). 13C-NMR (75 MHz, CDCl3), δ (ppm):
171.0, 75.5, 33.3, 27.5, 27.0, 22.6, 21.2, 14.0, 9.5.
3.2. Biocatalyzed Enzymatic Reactions
Racemic alcohols 1–6 (0.5 mmol) were added to a 50 mL Erlenmeyer flask containing 10 mL of
hexane (HPLC grade), 0.5 mL of vinyl acetate and 80 mg of immobilized lipase B from C. antarctica.
The reaction mixture was stirred in an orbital shaker (32 C, 150 rpm) until the consumption of the
reagents (Table 1). The mixture was filtered and the solvent evaporated. The residue was purified by
silica gel column chromatography using hexane and AcOEt producing in good yields the enantiopure
alcohols 1, 3–6 and acetates 7, 9–12 (Table 1). The progress of the reactions was monitored by
collecting samples (0.1 mL) and these were analyzed by GC-FID (1.0 µL) in a chiral capillary column.
The products were compared with the previously analyzed racemic mixtures. A similar procedure was
repeated to obtain the isolated yield (Table 1). In this case, a 50 mL Erlenmeyer flask was used
containing 10 mL of hexane, 1.0 mL of vinyl acetate, 100 mg of lipase CALB and 2.0 mmol of
racemic alcohols 1, 3–6.
3.3. GC-FID Analyses
The reaction products were analyzed using a Hewlett Packard (Palo Alto, CA, USA) model
HP-5890 gas chromatograph and using a Shimadzu GC 241 gas chromatograph equipped with an AOC
20i auto injector equipped with a Varian CP-Chiralsil-DEX -Cyclodextrin column (25 m × 0.25 mm
i.d.; 0.39 µm). The programs used for the GC-FID analyses of rac-alcohols 1–6 and rac-acetates 7–12
are described in Table 2. The injector and detector were maintained at 200 C, the split ratio of the
injector was 1:20, and the carrier gas was N2 at 60 kPa. The ee values of alcohols and acetates were
determined by GC-FID analyses (Figures 1–3 and Table 2).
3.4. Assignment of the Absolute Configuration
The optical rotation of the products from the biocatalytic reaction was measured in a Perkin-Elmer
(Waltham, MA, USA) model 241 polarimeter using a 1 dm cuvette and referenced to the Na–D line.
The absolute configurations of compounds (1, 4–6, 12) were determined comparing the specific
rotation signs measured for the products with that reported in the literature (Table 3) [14,25–29]. The
compounds 3, 7, 9–11 were suggested by empirical rule of Kazlauskas [5,24].

Molecules 2012, 17
Figure 1. Chiral analyses obtained by GC-FID chromatograms. (A) Racemic alcohol (±)-3.
8961
(B) Racemic acetate (±)-9. (C) Chromatograms of the kinetic resolution of rac-3 by lipase
CALB (20 min). (D) Chromatograms of the kinetic resolution of rac-3 by lipase CALB (40 min).
(E) Enantiopure acetate (S)-9 obtained after acetylating of alcohol (S)-3 by Ac2O and py.

Molecules 2012, 17
Figure 2. Chiral analyses obtained by GC-FID chromatograms. (A) Racemic alcohol (±)-4.
8962
(B) Racemic acetate (±)-10. (C) Chromatograms of the kinetic resolution of rac-4 by lipase
CALB (20 min). (D) Chromatograms of the kinetic resolution of rac-4 by lipase CALB
(40 min). (E) Chromatograms of the kinetic resolution of rac-4 by lipase CALB (60 min).
(F) Enantiopure acetate (S)-10 obtained after acetylating of alcohol (S)-4 by Ac2O and py.

Molecules 2012, 17
Figure 3. Chiral analyses obtained by GC-FID chromatograms. (A) Racemic alcohol (±)-6.
8963
(B) Racemic acetate (±)-12. (C) Chromatograms of the kinetic resolution of rac-6 by lipase
CALB (20 min). (D) Chromatograms of the kinetic resolution of rac-6 by lipase CALB
(40 min). (E) Chromatograms of the kinetic resolution of rac-6 by lipase CALB (60 min).
(F) Enantiopure acetate (R)-12 obtained after acetylating of alcohol (R)-6 by Ac2O and py.

Molecules 2012, 17
8964
Table 2. Programs used for identification of the alcohols 1–6 and acetates 7–12 by GC-FID analyses a.
Compounds Ti (C)
Tf (C)
60
ti (min)
tf (min)
r (C /min)
tr (min)
1
2
45
50
65
95
65
85
60
50
65
95
65
85
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
5
1
1
1
1
1
0.2
1
(R)-20.0; (S)-21.5
(R)-9.0; (S)-9.0
(R)-9.0; (S)-9.0
(R)-6.6; (S)-6.6
(R)-10.4; (S)-10.4
(R)-9.3; (S)-9.3
(R)-14.5; (S)-22.0
(R)-12.2; (S)-12.5
(R)-12.2; (S)-12.5
(R)-8.9; (S)-9.1
(R)-13.8; (S)-14.1
(R)-11.6; (S)-11.8
100
85
3
1
4
110
85
1
5
1
6
100
100
100
85
1
7
5
8
1
9
1
10
11
12
100
85
1
1
100
1
a
Chiral column: CP-Chiralsil-DEX -Cyclodextrin (25 m × 0.25 mm i.d.; 0.39 µm); Ti: initial
temperature; Tf: final temperature; ti: initial time; tf: final time; r: rate; tr: retention time; min: minutes.
Table 3. Experimental data of optical rotations of the aliphatic secondary alcohols and
acetates obtained by lipase B from Candida antarctica.
25
T
Compounds


D experimental


D literature
(S)-4-methylpentan-2-ol (1)
+4.40 (c 0.4; CHCl3)
R-enantiomer: −3.97 (neat) [29]
R-enantiomer: −18.4 (c 1.2; EtOH)[27]
(S)-5-methylhexan-2-ol (3)
(S)-octan-2-ol (4)
+8.68 (c 0.5; CHCl3)
+7.90 (c 0.6; CHCl3)
S-enantiomer: +10.3 (c 2.0; EtOH) [25]
S-enantiomer: +8.7 (c 1.0; CHCl3) [26]
R-enantiomer: −9.2 (c 7–8 g/100mL; CHCl3) [30]
S-enantiomer: +9.2 (c 7–8 g/100mL; CHCl3) [30]
S-enantiomer: +7.0 (c 1.0; pentane) [22]
S-enantiomer: +7.16 (c 1.38; pentane) [14]
R-enantiomer: −21.8 (c 2.0; CHCl3) [27]
(S)-heptan-3-ol (5)
+8.24 (c 0.2; CHCl3)
−8.06 (c 0.8; CHCl3)
(R)-oct-1-en-3-ol (6)
(R)-4-methylpentan-2-yl acetate −7.10 (c 0.8; CHCl3)
(7)
(R)-octan-2-yl acetate (10)
(R)-heptan-3-yl acetate (11)
(S)-oct-1-en-3-yl acetate (12)
−2.96 (c 0.5; CHCl3)
+1.79 (c 0.7; CHCl3)
−12.07 (c 0.9; CHCl3) S-enantiomer: −11.5 (c 2.8; CHCl3) [22]
3.5. Enantiomeric Ratio (E)
The enantiomeric ratio (E) for the kinetic resolutions of racemic alcohols was calculated for
catalysed reactions by equations formulated by Sih and coworkers [31]. The calculation of the
selectivity of the kinetic resolution expressed as enantiomeric ratio (E) for irreversible reactions was
obtained by a computer program described by Faber [32].
4. Conclusions
In summarized, we have reported the chemoenzymatic transesterification of aliphatic rac-secondary
alcohols under mild conditions. The lipase CALB-catalysed enantioselective esterification reaction

Molecules 2012, 17
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afforded the alcohols (1, 3–6) and acetates (7, 9–12), in accordance with the Kazlauskas rule, yielding
high enantiomeric excesses (>99% ee).Various substituent groups (isobutyl, isopentyl, n-butyl,
n-pentyl, n-hexyl, ethyl, methyl, vinyl) attached to the stereogenic center were efficiently accepted by
lipase CALB.
Acknowledgments
The authors wish to thank Prof. Timothy John Brockson (Universidade Federal de São Carlos -
UFSCar) for optical rotation measurements in a Perkin-Elmer 241 polarimeter (Waltham, MA, USA).
LCR thanks CAPES and HVF thanks CNPq for the scholarships. ALMP gratefully acknowledges
CNPq and FAPESP for their financial support. The authors also wish to thank Novo Nordisk
(Curitiba-Paraná, Brazil) for donating the immobilized CALB (Novozym 435®).
References
1. Gotor-Fernandez, V.; Vicente G. Use of Lipases in Organic Synthesis. In Industrial Enzymes—
Structure, Function and Applications; Polaina, J., MacCabe, A.P., Eds.; Springer: Weinheim,
Germany, 2007; Chapter 18, p. 642.
2. Liu, H.-L.; Anthonsen, T. Enantiopure building blocks for chiral drugs from racemic mixtures of
secondary alcohols by combination of lipase catalysis and Mitsunobu esterification. Chirality
2002, 14, 25–27.
3. Larios, A.; García, H.S.; Oliart, R.M.; Valerio-Alfaro, G. Synthesis of flavor and fragrance esters
using Candida antarctica lipase. Appl. Microbiol. Biotechnol. 2004, 65, 373–376.
4. Barbosa, O.; Ortiz, C.; Torres, R.; Fernandez-Lafuente, R. Effect of the immobilization protocol
on the properties of lipase B from Candida antarctica in organic media: Enantiospecifc
production of atenolol acetate. J. Mol. Catal. B Enzym. 2011, 71, 124–132.
5. Bornscheuer, U.T.; Kazlauskas, R.J. Hydrolases in Organic Synthesis: Regio- and Stereoselective
Biotransformations, 2nd ed.; Wiley: Weinheim, Germany, 2006.
6. Jaeger, K.E.; Eggert, T. Lipases for biotechnology. Curr. Opin. Biotechnol. 2002, 13, 390–397.
7. Ghanem, A. Trends in lipase-catalyzed asymmetric access to enantiomerically pure/enriched
compounds. Tetrahedron 2007, 63, 1721–1754.
8. Krishna, S.H.; Karanth, N.G. Lipase and lipase-catalyzed esterification reactions in non-aqueous
media. Catal. Rev. Sci. Eng. 2002, 44, 499–591.
9. Monteiro, C.M.; Lourenço, N.M.T.; Afonso, C.A. Separation of secondary alcohols via enzymatic
kinetic resolution using fatty esters as reusable acylating agents. Tetrahedron Asymmetry 2010,
21, 952–956.
10. Kim, M.-J.; Choi, Y.K.; Choi, M.Y.; Kim, M.J.; Park, J. Lipase/ruthenium-catalyzed dynamic
kinetic resolution of hydroxy acids, diols, and hydroxy aldehydes protected with a bulky group.
J. Org. Chem. 2001, 66, 4736–4338.
11. Koh, J.H.; Jung, M.J.; Kim, M.-J.; Park, J. Enzymatic resolution of secondary alcohols coupled
with ruthenium-catalyzed racemization without hydrogen mediator. Tetrahedron Lett. 1999, 40,
6281–6284.

Molecules 2012, 17
8966
12. Ghanem, A.; Aboul-Enein, H.Y. Application of Lipases in Kinetic Resolution of Racemates.
Chirality 2005, 17, 1–15.
13. Muralidhar, R.V.; Marchant, R.; Nigam, P. Lipases in racemic resolutions. J. Chem. Technol.
Biotechnol. 2001, 76, 3–8.
14. Ohtani, T.; Nakatsukasa, H.; Kamezaw, M.; Tachibana, H.; Naoshima, Y. Enantioselectivity of
Candida antarctica lipase for some synthetic substrates including aliphatic secondary alcohols.
J. Mol. Catal. B Enzym. 1998, 4, 53–60.
15. Zarcula, C.; Corîci, L.; Croitoru, R.; Péter, F. Kinetic Resolution of Secondary Alcohols in
Lipase-Catalyzed Transesterification Reactions. In Annals of West University of Timisoara Series
of Chemistry IX. Proceedings of ISYPMR 2007-ACM-V–The IX International Symposium
“Young People and Multidisciplinary Research”, Timisoara, Romanian, 2007; Volume 16,
pp. 107–112.
16. Chojnacka, A.; Obara, R.; Wawrzenczyk, C. Kinetic resolution of racemic secondary aliphatic
allylic alcohols in lipase-catalyzed transesterification. Tetrahedron Asymmetry 2007, 18, 101–107.
17. Rocha, L.C.; Rosset, I.G.; Luiz, R.F.; Raminelli, C.; Porto, A.L.M. Kinetic resolution of
iodophenylethanols by Candida antarctica lipase and their application for the synthesis of chiral
biphenyl compounds. Tetrahedron Asymmetry 2010, 18, 926–929.
18. Ferraz, H.M.C.; Bianco, G.G.; Teixeira, C.C.; Andrade, L.H.; Porto, A.L.M. Enzymatic resolution
of α-tetralols by CALB-catalyzed acetylation. Tetrahedron Asymmetry 2007, 18, 1070–1076.
19. Raminelli, C.; Comasseto, J.V.; Andrade, L.H.; Porto, A.L.M. Kinetic resolution of propargylic
and allylic alcohols by Candida antarctica lipase (Novozyme 435). Tetrahedron Asymmetry 2004,
15, 3117–3122.
20. Ferreira, H.V.; Rocha, L.C.; Severino, R.P.; Viana R.B.; Da Silva, A.B.F.; Porto, A.L.M.
Enzymatic Resolution of Racemic Sulcatol by Lipase from Candida antarctica in a Large Scale.
J. Iran. Chem. Soc. 2010, 7, 883–889.
21. Komisarski, M.; Kaczmarska, Z.; Kusmierek, J.T. Practical highly enantioselective synthesis of
(R)- and (S)-(E)-4-hydroxynon-2-enal. Acta Biochem. Polonica 2009, 56, 189–193.
22. Felluga, F.; Forzato, C.; Ghelfi, F.; Nitti, P.; Pitacco, G.; Pagnoni, U.G.; Roncaglia, F. Atom
transfer radical cyclization (ATRC) applied to a chemoenzymatic synthesis of Quercus lactone.
Tetrahedron: Asymmetry 2007, 18, 527–536.
23. Bornscheuer, U.; Schapohler, S.; Scheper, T.; Schiigerl, K. Influences of reaction conditions on
the enantioselective transesterification using Pseudomonas cepacia lipase. Tetrahedron: Asymmetry
1991, 2, 1011–1014.
24. Jing, Q.; Kazlauskas, R.J. Determination of absolute configuration of secondary alcohols using
lipase-catalysed kinetic resolutions. Chirality 2008, 15, 724–735.
25. Maywald, M.; Pflatz, A. Chromatography-free enzymatic kinetic resolution of secondary
alcohols. Synthesis 2009, 21, 3654–3660.
26. Wang, Y-F.; Lalonde, J.J.; Momongan, M.; Bergbreiter, D.E.; Wong, C-H. Lipase-catalysed
irreversible transesterifications using enol esters as acylating reagents: Preparative enantio- and
regioselective syntheses of alcohols, glycerol derivatives, sugars and organometallics. J. Am.
Chem. Soc. 1988, 110, 7200–7205.

Molecules 2012, 17
8967
27. Graylin, P.M.; Knox, J.R. (R)-4-Methyl-2-pentyl acetate from Eucalyptus loxophleba. J. Nat. Prod.
1991, 54, 295–297.
28. Dhokte, U.P.; Pathare, P.M.; Mahindroo, V.K.; Brown, H.C. Chiral synthesis via organoboranes.
48. Efficient synthesis of trans-fused bicyclic and cyclic ketones and secondary alcohols in high
optical purities via asymmetric cyclic hydroboration with isopinocampheylchloroborane etherate.
J. Org. Chem. 1998, 63, 8276–8283.
29. Yaozhong, J.; Yong, Q.; Aiqiao, M.; Zhitang, H. Asymmetric synthesis XXI. Enantioselective
reduction of ketones catalysed by new (4S,5R)-4,5-diphenyl-1,3,2-oxazaborolidine. Tetrahedron:
Asymmetry 1994, 5, 1211–1214.
30. Hirata, H.; Ikushima, M.; Watanabe, M.; Kawauchi, K.; Miyagishi, M.; Chen, Y-G.; Yanagishita,
H. Kinetic resolution of optically pure 3-alkanol by Pseudomonas cepacia lipase-catalyzed
transesterification with vinyl acetate in organic solvent. J. Oleo. Sci. 2003, 52, 375–386.
31. Chen, C.-S.; Fujimoto, Y.; Girdaukas, G.; Sih, C.J. Quantitative analyses of biochemical kinetic
resolutions of enantiomers. J. Am. Chem. Soc. 1982, 104, 7294–7299.
32. Faber, K.; Kroutil, W. Bioorganic Chemistry. Available online: http://borgc185.kfunigraz.ac.at/
index.htm (accessed on 18 April 2012).
Sample Availability: Samples of the compounds are available from the authors.
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