ENZYMIC SYNTHESES OF DOUBLY BRANCHED CYCLOMALTOHEPTAOSES THROUGH THE REVERSE ACTION OF Pseudomonas ISOAMYLASE

Article abstract of DOI:10.1016/0008-6215(88)84060-6

Two and three new cyclomaltoheptaose (β-cyclodextrin, cG₇) derivatives, respectively, were identified among the products obtained by the action of Pseudomonas isoamylase on maltose and maltotriose, and cG₇.They were 6^A,6^D-di-O-α-maltosyl-cG₇ and 6-O-α-(6²-O-α-maltosyl)maltosyl-cG₇, and 6^A,6^D-di-O-α-maltotriosyl-cG₇, 6-O-α-(6³-O-α-maltotriosyl)maltotriosyl-cG₇, and 6-O-α-(6²-O-α-maltotriosyl)maltotriosyl-cG₇.In addition, 6¹- and 6²-O-α-maltosylmaltose were identified as mutual condensation products of maltose.Maltose was the smallest substrate to act as both an acceptor and a donor for the action of Pseudomonas isoamylase.