IMMOBILIZATION OF PROTOCATECHUATE 3,4-DIOXYGENASE FROM PLEUROTUS OSTREATUS ON ACTIVATED POROUS GLASS BEADS

Article abstract of DOI:10.1016/0031-9422(88)80651-4

Protocatechuate 3,4-dioxygenase (protocatechuate: oxygen 3,4-oxidoreductase, EC 1.13.11.3) purified from the wood-degrading fungus Pleurotus ostreatus was immobilized on glass beads.The per cent attached protein was 78 with a retained enzymatic activity of 90percent.The basic properties of the immobilized enzyme were tested and compared with those of a soluble form.Immobilized dioxygenase was stable over wide ranges of pH and temperature.Moreover, the immobilization brought about a slight increase of its specific activity.Key Words Index - Pleurotus ostreatus; protocatechuate 3,4-dioxygenase; immobilization; porous glass beads.