Combined effects of a buffer and solvent on tetramethylpyrazine formation in a 3-hydroxy-2-butanone/ammonium hydroxide system

Article abstract of DOI:10.1271/bbb.61.1013

A phosphate buffer was found to significantly promote tetramethylpyrazine (TMP) formation in an acetoin (3-hydroxy-2-butanone)/ammonium hydroxide system. The effect of the phosphate ion on TMP formation was additive in the range of 0.05-0.2 M. The change in pH value of the system reveals that a proton-coupled redox type of reaction occurred during TMP formation. Phosphate serves both as proton donor and acceptor to facilitate proton transfer during the Schiff base formation between ammonia and 3-hydroxy-2-butanone. Protic solvents, methanol, and ethanol, were found to attract the water released from the system. The combination of a phosphate buffer and protic solvent led to the completion of TMP formation. The TMP formation mechanism in a phosphate buffer (pH 7.2) is proposed.

Full text of DOI:10.1271/bbb.61.1013

This article was downloaded by: [University of Auckland Library]
On: 08 October 2014, At: 18:41
Publisher: Taylor & Francis
Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer
House, 37-41 Mortimer Street, London W1T 3JH, UK
Bioscience, Biotechnology, and Biochemistry
Publication details, including instructions for authors and subscription information:
http://www.tandfonline.com/loi/tbbb20
Combined Effects of a Buffer and Solvent on
Tetramethylpyrazine Formation in a 3-Hydroxy-2-
butanone/Ammonium Hydroxide System
Tzou-Chi Huanga
a Department of Food Science and Technology, National Pingtung Polytechnic Institute,
Pingtung 912, Taiwan, Republic of China
Published online: 12 Jun 2014.
To cite this article: Tzou-Chi Huang (1997) Combined Effects of a Buffer and Solvent on Tetramethylpyrazine Formation
in a 3-Hydroxy-2-butanone/Ammonium Hydroxide System, Bioscience, Biotechnology, and Biochemistry, 61:6, 1013-1015,
DOI: 10.1271/bbb.61.1013
To link to this article: http://dx.doi.org/10.1271/bbb.61.1013
PLEASE SCROLL DOWN FOR ARTICLE
Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained
in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no
representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of
the Content. Any opinions and views expressed in this publication are the opinions and views of the authors,
and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied
upon and should be independently verified with primary sources of information. Taylor and Francis shall
not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other
liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or
arising out of the use of the Content.
This article may be used for research, teaching, and private study purposes. Any substantial or systematic
reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any
form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http://
www.tandfonline.com/page/terms-and-conditions

NII-Electronic Library Service

NII-Electronic Library Service

NII-Electronic Library Service